Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A29 F36 I0 R1 179 9550.8 8038540 98.3% 7901884 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,547,110 G→C 18.6% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,547,1100GC18.6% 81.1 / 2.8 60intergenic (+22/‑12)CIW80_RS07710/CIW80_RS07715tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base G (32/16);  new base C (8/4);  total (40/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGG  >  NZ_CP022686/1546971‑1547237
                                                                                                                                           |                                                                                                                               
ctgaaCTAATTATAATTTGTTGTTTTTAAGCAGTACTGTGACGCGGGGTTGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGATATAAAg                                                                                                                                 <  7:934145/137‑1 (MQ=14)
ctcAACCAATTAAACTTTGATGAAGTAACCCAGTACTGGGACGCGGTGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                 <  5:147859/138‑1 (MQ=17)
cGCCACCAATTATATATTGAAGAATTTAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGTCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                 <  1:896257/140‑1 (MQ=17)
cGCAACCAATTATAATTTGATGAAGTTAAGCAGTACGGGGACGCGGGGTGGAGCTGCAGGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCTGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                 <  7:155201/140‑1 (MQ=17)
cGCAACCAATTAAAATTTTCTGTAGATAAGCAGTCCGGGGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                 <  5:958731/140‑1 (MQ=17)
     ccAATTAAAATTTTATGAAGTATAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTa                                                                                                                            <  3:843440/140‑1 (MQ=255)
        aTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCCACCAATGAAAATTTGATGATAAAAAGCGGTACgg                                                                                                                         >  7:311400/1‑140 (MQ=17)
             atagacttgaAGAAACGCATTACTCTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGATCATAACCCGAAGGTCGTCGGTTCTAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGAcg                                                                                                                    <  1:1016835/133‑1 (MQ=14)
             aaTTTCATCAAGTAAAGCAGTCCGGGGCAGCGGGGTGTAGCAGCATGTTAGCGCGGCGGGCTCATACCCCGAAGGTCGTCGGTTCAAATCCGGCCCGCGCAACCAATCAAAATTTGATTAAATAAAGCAGTACGGTGAcg                                                                                                                    <  7:220705/140‑1 (MQ=14)
                ttgttgAAGTCTCGCATTACGGTGACGCGGGGGGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCgg                                                                                                                 <  3:898721/140‑1 (MQ=14)
                gtgatgaATTAAACCAGTACTGGGAAGCGGTGTGGAGCAGCATGGTAGATCGTCGGGCTCATAACCCGCAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCgg                                                                                                                 <  3:251058/139‑1 (MQ=14)
                       agatcaccAGTACTGTCACGCTGGGTGGATCAGCAAGGTAGATCTTCGGGCTCATAACCCGAATGTCGTAGGTGCAAATCCTGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGag                                                                                                          <  2:1028852/133‑1 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGTTTCAAATCAGGCCCCCGCAACCAATAAAAAATTGATGAAATAAACAAGTAAGCTGAGAATGGGTGGagcagctttaga                                                                                                >  4:998470/1‑134 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGGAACCAATCAAATTTTGTTGATATAAAGCTCAACGCTGAAGCGGGGTGGAGCAGCCTGGtt                                                                                                >  1:347468/1‑139 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCTAATATTGAGGAAATAAAGCAGAACGGTGCCTCGGGGTGGAGCATGCTGGtt                                                                                                >  4:199327/1‑139 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAGAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGtt                                                                                                >  3:848403/1‑139 (MQ=255)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAAAAAGCAGTACGGTGCCCCGGGGTGGCGCAGACTGGTa                                                                                                >  3:683981/1‑140 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATATAAAATTGATGAAAAAAAGCTGAAAGCTGCATAGGGGTGGAGCATCGTGGtt                                                                                                >  4:467561/1‑139 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATATTTGAGGATATAAACCAGTACGGTGCCGAGGGGTGGCGCATACTgggt                                                                                                >  4:962469/1‑138 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAATTTATATGAAATAAAGCAGAACGGTGCCGCGGGGTGGAGCAGAATGGTa                                                                                                >  3:82785/1‑140 (MQ=11)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAAATTGATCAAAAAAACCACAACGCTGAAGCGGGGTTGAGCAGCCTGGTa                                                                                                >  4:354363/1‑140 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGATCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAAATTCATTAAAAAAAGCAGAACGGTGAATATGGGTCGAGCAGAATGGtt                                                                                                >  2:918332/1‑139 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGACGGGCTCATCAACACATGGGCGTCGGTACAATGCCGGCCCCCGCCTCCCATCAAAATTTGATGAAATAAAGAAGTACGGGGACGCGGGGTGGAGCTGCCGGGtg                                                                                                >  5:247942/1‑139 (MQ=14)
                                   cGGTGACGCGGGGTGGAGCAGCTTGGTAGCTCGTCGGGCGGATATCCCGAAGGTCGTCTGTTCAGATCAGGCCCCCGCAACCAATCAAGATTAGTTGAAATAAAGAAGGACTGTGCCGCGCGGTGGCTCAGCCTGGTAgg                                                                                              >  7:593571/1‑139 (MQ=17)
                                      tGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGACATAAACCAGTACGGTGCCTCGGGGTGGAGCAGCCTGGTAGCtcg                                                                                           >  7:288221/1‑140 (MQ=11)
                                         cgagttgTGGATCAGACTGGTCGCTATTCGCTCTCTAAACCCGAATCGCGTCTGGTCCAATACTGCCCCCGCCACCAAAAAAACTTACAAGAAATTTTCCCTTACCCTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg                                                                                        <  2:576704/134‑1 (MQ=255)
                                         atctgggTGGTGCAGCTTTGTATCTGGGGTGTCTAATAACCCGAAGGGCGGCTGTTGTAATCCTGCCCGCGCAACCAATAAAAATTAGATGAATTTTAGCCGTACTGGGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg                                                                                        <  6:950371/136‑1 (MQ=255)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTTATCTTAGAAACCAGTACGGTGACGCGGGGTGGCGCAGCGTGGTTGAtagtcgtg                                                                                      >  1:818891/1‑138 (MQ=11)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGTAAAAAAGCAGTACGGTGCGGCGGGCTGGCGCAGCATGGTAGCTGATCggg                                                                                      >  2:285498/1‑140 (MQ=11)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGATAGAAACCCGTAAGGTGCCTCTGGGTGGAGCAGCCTGGGAGATAGTCggg                                                                                      >  2:368984/1‑140 (MQ=14)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAATTTTGATGAAAAAAAGAGCTACGGTGAGTGTGGCTGGAGCTGACTGGTAGATCGTCggg                                                                                      >  3:371067/1‑140 (MQ=11)
                                             gggTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAAATTTTTTGTAATAAAGCAGTAAGGTGACGATGGGTGGCGCAGAATGGTAGCTcggcggtcg                                                                                    >  4:738002/1‑137 (MQ=11)
                                               gTGGATCAGCCTGGTAGCTCGGAGGGCTAAAAACCCGAAGGGCGTCGGTTCCAATACCGCCCCCGCAACCAATCATATATTCTTGAAATAAAGCACTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                  <  7:392058/140‑1 (MQ=11)
                                               gTGGAGCAGCGTGTTCGCTCGTAGGTAGCAAAACCCGAAGGTCGTCGCTGCAAAACCGCCCCCCTCAACCAACAAAAATTTGCTGAAATAACGCCCTACCGTGACGCGTGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                  <  4:231213/140‑1 (MQ=2)
                                                tgctccagccTGGTCTCTCTGCGGCCTAACAACCCGAAGTTGGTAGGTTCTAATCCGGACCCCTGAACCAATCATAATCGGATGAAATGAAGCCGTCCTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCTGGCTCAt                                                                                 <  8:396331/135‑1 (MQ=2)
                                                tGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                 <  3:927502/92‑1 (MQ=40)
                                                tGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                 >  4:927502/1‑92 (MQ=40)
                                                tGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATATAATATTGATGATAAAATCCAGTACGCTGCGGCGGGGTGGCGCTGCATGGGAGATCGTCGTGcggat                                                                                 >  3:20581/1‑136 (MQ=2)
                                                        caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCTAAATTTTAGGTTATAAAGCTCTACGGTGACGCGGGGTGGCGCAGCCTTGTTGGTCGACGGGCTCTAACCCCGaa                                                                         >  5:627883/1‑140 (MQ=14)
                                                        caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAAAAACCAGTACGGTGCCGCGGGGTGGAGCAGGCTGGTAGCTAGTCGGGCTCATAACCGGaa                                                                         >  7:478908/1‑140 (MQ=14)
                                                        caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGACGGGCTCATACCCCTaa                                                                         >  7:159568/1‑140 (MQ=255)
                                                             tcgCTCTTCTGTCTCATAACCCGAAGGGCGGCGGTGCAAATCAGGCCCCCGCAACCAATCATAATGAGTTGAATTAACCGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                                                                    <  3:649242/138‑1 (MQ=11)
                                                             tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAAATTTATTGATATAAAGCAGTACGGTGCAGCGGGGTGGAGCGGCGTGGTAGCTGGTCGGGCGCATCAACCGAAGgtcg                                                                    >  5:506118/1‑140 (MQ=11)
                                                              agagttgcgGTCTCTTACCACGAAGCTCTTCTGTTCTTATCAGGCACGCCCCACCAATCAAATTTTTATGAATTAAAGGAGTCCTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                   <  4:260361/133‑1 (MQ=2)
                                                              aGCTCGTCGGGCGCATACACCGATGGACTTCGTACCAACTCCGCCCCCCGCAACCACTAAAACTTTGATGAAAACACCCATTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                   <  2:527213/140‑1 (MQ=2)
                                                              aGCTCGGAGGTCTCATAACCCGAAGGGCTTCGGGGCAAATACGGCCCCCGCAACCAATCATAATTTGATGAATTAAAGCAGTACGGTGACGCGGTGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                   <  5:297783/140‑1 (MQ=11)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCTATAATTGAGGAGATAAAGCGGTACGGTGCAGCTGTGTGGAGCAGGGTGGTTGTTAGACGGGCAGATAACCCGTAGGtcgtcgct                                                               >  6:518688/1‑138 (MQ=2)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTgg                                                                                                            >  8:483558/1‑95 (MQ=37)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTgg                                                                                                            <  7:483558/95‑1 (MQ=37)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGATATAAAGCAGTACGGTGACGCGGGGTGGCGCAGGCTGGGAGCTCGGCGGTCTCAAACCCCGAAGGTCGTCGGt                                                               >  1:414625/1‑140 (MQ=11)
                                                                     cgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAAATTTGATGAAATAAAGCAGTACGGTGCCGGTGGGTGGAGCAGCCTGGTAGCTCGTCGGTCTCATAACCCGAAGGTCGTCGGTTCa                                                            >  4:799560/1‑140 (MQ=11)
                                                                         cTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAATATTTGATCAGATAAAGCAGTAAGGTGCCACGGGGTTGCGCAGAATGCTATCTATACGGGCTGATAACCAGACTGTCGTCGGTTaaacta                                                        >  2:443291/1‑137 (MQ=2)
                                                                              aaCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGAAAAAAAGCAGTACGGTGACGCGGGGTGGCGCAGCCTGGTAGCTCGTCGGTCTAATAACCCGACGGTCGTCCGTGAGACTCCGGcc                                                   >  3:550778/1‑140 (MQ=11)
                                                                                  cGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAACTCCGGcccccc                                               >  3:362394/1‑139 (MQ=19)
                                                                                    aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAATATTTGATGAAAAAAAGCAGTACGGTGACGCGGGGTTGAGCAGACTGGTTGCTAGGCGGGCAGATCACCCGAAGGTCGAGGGTGCGAATCCGGCCCACGcc                                             >  8:654140/1‑139 (MQ=11)
                                                                                    aaGGCCGTCTGTTCAAATACTCCCGCCGCAACCAATCAAAATTTTCTGAAATTAACCAGTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCa                                             <  3:694848/140‑1 (MQ=11)
                                                                                        tcgtcgGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAg                                                                                                                              >  4:1008937/1‑55 (MQ=255)
                                                                                        tcgtcgGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAg                                                                                                                              <  3:1008937/55‑1 (MQ=255)
                                                                                           tcgGTTCAAATCCGGCCCCCGCAACCAATCAAAAATTGATGAAAAAAAGCAGAACGCTGCCGCTGGGTTGAGCAGACTGGTAGATAGTCGGGCGCATAACCAGAAGGTCATCCCTTCAAATACGACCCAACCAACAAAtt                                      >  8:385536/1‑140 (MQ=2)
                                                                                                         gCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCGTGGTAGCTCGTCGGGCTCATAACGCGAAGGTCGTCGGTTCAAATCAGGCCCCCGCAAACAATTTATTAGAAAGCTgc                        >  7:190069/1‑139 (MQ=14)
                                                                                                         gCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGACGGGCTCATAACCAGAAGGTCGTCGGTTCAACTCAGGGCCCCGCAACCAATTGATTAGAatgatgc                        >  6:196623/1‑139 (MQ=14)
                                                                                                              cGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                      <  6:268625/73‑1 (MQ=255)
                                                                                                              cGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                      >  5:268625/1‑73 (MQ=255)
                                                                                                                       tCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAgct          >  4:348914/1‑140 (MQ=255)
                                                                                                                               tgatgaAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTGCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTgg  >  7:697873/1‑140 (MQ=255)
                                                                                                                                     aaTAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                     >  2:216818/1‑35 (MQ=40)
                                                                                                                                     aaTAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                     <  1:216818/35‑1 (MQ=40)
                                                                                                                                           |                                                                                                                               
CGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGG  >  NZ_CP022686/1546971‑1547237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A