Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A29 F11 I0 R1 249 11230.8 10274356 98.4% 10109966 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,110,457 T→G 17.0% K12N (AAA→AAC)  rplM ← 50S ribosomal protein L13

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,110,4570TG17.0% 398.0 / 25.5 243K12N (AAA→AAC) rplM50S ribosomal protein L13
Reads supporting (aligned to +/- strand):  ref base T (71/122);  new base G (44/3);  total (117/126)
Fisher's exact test for biased strand distribution p-value = 2.06e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

TTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAAT  >  NZ_CP022686/2110320‑2110593
                                                                                                                                         |                                                                                                                                          
            atgTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTATTACG‑Gtttat                                                                                                                               >  4:384004/1‑136 (MQ=255)
            atgTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTTCG‑Gtttgt                                                                                                                               >  3:1291582/1‑136 (MQ=255)
                 aTCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTAAG‑GTTT‑CTGGTTTat                                                                                                                       >  8:240389/1‑139 (MQ=255)
                  tCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTTAG‑GTTT‑GTGGTTTatg                                                                                                                      >  5:1199698/1‑138 (MQ=255)
                      cGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCACTCGCGTTTTAAC‑GTTT‑CTGGTTTAGCTGTa                                                                                                                  >  8:498824/1‑140 (MQ=255)
                      cGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCACAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACT‑GTTT‑CGGGTTTAGCTg                                                                                                                    >  3:676714/1‑138 (MQ=255)
                        gTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTa                                                                                                                  >  4:517285/1‑138 (MQ=255)
                            cTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGCCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGtt                                                                                                            <  6:412671/140‑1 (MQ=255)
                             tACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGt                                                                                                             <  3:949525/138‑1 (MQ=255)
                             tACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGGTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGttt                                                                                                           <  2:1121224/140‑1 (MQ=255)
                                gTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCa                                                                                                        >  8:140255/1‑140 (MQ=255)
                                   cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGGTTAAG‑GTTTGATGTTTTTGC‑GTAAAAGTGTTAAttt                                                                                                     >  6:257172/1‑139 (MQ=255)
                                   cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGGCAACAACATACCAGTCGCGGTTTTAG‑GTTT‑CTGGTTTTGCTGTAAAAGTTTTCATTa                                                                                                     >  5:1149548/1‑140 (MQ=255)
                                   cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTAACGGTCGCGTCAAAAACATACCAGTCGCGTTTTACGAGTTA‑GTGG‑TTAGCTTATAAATTTTTCATTa                                                                                                     >  6:863301/1‑140 (MQ=255)
                                   cGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGAAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTAAC‑GTTT‑GTGGTTTTGCTGTAAAAGTTTTCtttt                                                                                                     >  5:291410/1‑139 (MQ=255)
                                        tgtATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTaaaa                                                                                                  <  3:1069624/138‑1 (MQ=255)
                                        tgtATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTAGG‑GTTT‑CGGGTTTAGCTTTATAAGTTTTCATTAAAAgg                                                                                                >  1:1295920/1‑139 (MQ=255)
                                        tgtATTCCGATTTGTGATTACCTATCAGGCGACGAGACAGTTCAGTAGCCAGACGGCCCAGAGTTTTAAAGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAATTTTTCATTaaaa                                                                                                  <  3:741882/138‑1 (MQ=255)
                                          tATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑ATGGTTTAGCTGTATAAGTTTTAATTATAAGCtt                                                                                              >  2:850970/1‑140 (MQ=255)
                                            ttCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCCACAACATACCAGTCGCGTTTTACG‑GTTT‑GAGGTTTAGCTGTAAAAGTTTTAATTAAAAGcgt                                                                                              >  3:495799/1‑136 (MQ=255)
                                            ttCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTAc                                                                                            <  6:1185563/140‑1 (MQ=255)
                                             tcagatttGTTCTTCACGCGCAGGCGAAGAGCCAGTTCAGTAGACAGACGGCACAGAGTTTTACCGGTCGCGTCAACAAAATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTa                                                                                             <  3:695583/133‑1 (MQ=255)
                                             tCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTAcc                                                                                           <  2:992494/140‑1 (MQ=255)
                                             tCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTAcc                                                                                           <  5:362329/140‑1 (MQ=255)
                                                gCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGGTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaa                                                                                        >  8:846926/1‑140 (MQ=255)
                                                gCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACG‑GTTT‑GTGGTTTTGCGGTTAAAGTTTTGATTTAAAGCGTTCCCaa                                                                                        >  1:247601/1‑140 (MQ=255)
                                                  tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaata                                                                                      >  7:937404/1‑140 (MQ=255)
                                                  tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaa                                                                                        <  3:1115342/138‑1 (MQ=255)
                                                  tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑ATGGGTTAGCTTTAAAAGTTTTCATTAAAAGCTTACCCaata                                                                                      >  8:646169/1‑140 (MQ=255)
                                                  tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTTAT‑GTTT‑CTGGGTTAGCTGTATAAGTTTTCATTAAAAGCTGACCCatta                                                                                      >  6:906509/1‑140 (MQ=255)
                                                  tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACG‑GTGT‑AGGGGTTAGCTGGAAAAGTTTTAATTAAAATCTGAACCaata                                                                                      >  5:433658/1‑140 (MQ=255)
                                                  tttGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTGTACG‑GGTT‑CTGGTTTAGCTGTAAAAGTTTTAATTAAAAGCTTACCCaata                                                                                      >  2:411793/1‑140 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTTCG‑GTTT‑GTGGGTTAGCTGTAAAATTTTTCATTAAAAGCTTACCCaata                                                                                      >  4:1132292/1‑138 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTTGCTGTAAAAGTTTTCATTAAAATCTTACCCaataaa                                                                                    >  5:185542/1‑140 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCaata                                                                                      >  4:687329/1‑138 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GGTT‑CTGGTTTAGCTGTAAAATTTTTCATTAAAAGCTTACCCaata                                                                                      >  4:642627/1‑138 (MQ=255)
                                                    tgtgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGGCAACAAAATACAAGTCGCGTTTTTAG‑GTTT‑CTGGTTTAGATGTAAAAGTTTTAATTAAAAGATTACCCaaaaaa                                                                                    >  6:458913/1‑140 (MQ=255)
                                                      tgCTTACCGCGCAGGCGACGCGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACG‑GGTT‑CTGGTTTTGCTGTAAAAGTTTTAATTAAAAGGTTAACCaataaata                                                                                  >  8:681691/1‑140 (MQ=255)
                                                      tgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTTTAAAAGTTTTAATTAAAAGCTTAACCaaaaaa                                                                                    >  4:263672/1‑138 (MQ=255)
                                                      tgCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCCCGATTTACG‑GTTT‑CTTGTTTAGCTGTTAAAGTTTTCATTAACAGCTTACCCaataaa                                                                                    >  4:797523/1‑138 (MQ=255)
                                                          tACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTa                                                                              <  2:735773/140‑1 (MQ=255)
                                                           aCCGCGCAGGCGACGAGCCAGTTCAGTATCCAGACGGCCCAGAGTTGTACAGGACGATACAAAAAAAAAAAAGAAGCGTTTTAAG‑GTTT‑CAGGTTTAGCTATAAAAGTTTTCATTAAAAGCTTAGCCCATAAAAAGTTaa                                                                             >  6:1230396/1‑139 (MQ=255)
                                                           aCCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GGTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAAAAAAAAGttta                                                                             >  5:755647/1‑138 (MQ=255)
                                                           aCCGCGAAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACAGGTCGCGTCAACAACTTACCAGTCGCGTTTTTCG‑GTTT‑ATGGTTTAGGTGTAAAAGTTTTCATTAAAAGGTTACCCAATTAATATTTaa                                                                             >  2:768314/1‑139 (MQ=255)
                                                            ccGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTaca                                                                            <  1:221491/140‑1 (MQ=255)
                                                             cgcgCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTATAAGTTTTCATTAAAAGCGTACCCAATAAATAGTTaa                                                                             >  4:192820/1‑137 (MQ=255)
                                                              gcgcAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACg                                                                          <  7:849443/140‑1 (MQ=255)
                                                              gcgcAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACg                                                                          <  5:875468/140‑1 (MQ=255)
                                                                gcAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTAAACg                                                                          >  4:1116961/1‑138 (MQ=255)
                                                                    gCGACGAGCCAGTTCAGTAGCCAGACGGCCCATAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAATTTTTAATTAAAATCTTAACCAATAAATAGTTAAACGTTGGTg                                                                    >  5:819967/1‑140 (MQ=255)
                                                                             cAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCaa                                                           <  5:141617/140‑1 (MQ=255)
                                                                             cAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCaa                                                           <  2:360092/140‑1 (MQ=255)
                                                                               gTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAAc                                                         <  1:1037069/140‑1 (MQ=255)
                                                                                  cAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTc                                                      <  1:784743/140‑1 (MQ=255)
                                                                                     tAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTc                                                   <  7:753426/140‑1 (MQ=255)
                                                                                     tAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACG‑GTTT‑ATGGTTTATCTGTAAAAATTTTAATTAAAAGATTAACCAATAAATATTTAAACGTTTGTGAACACCCCAACGTcata                                                   >  7:188575/1‑137 (MQ=255)
                                                                                     tAGCCAGACGGCCCAGAGTTCTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCtta                                                   >  2:955119/1‑139 (MQ=255)
                                                                                       gCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTAAG‑GGTT‑GTGGGTTTGCGGTTAAAGTTTTCATTTAAAGCTTTCCCAATTAATATTTACACGTTGGTGAACACCCAAAccgctta                                                   >  3:378281/1‑132 (MQ=255)
                                                                                       gCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCACTCGCGGTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTc                                                   <  3:885764/138‑1 (MQ=255)
                                                                                        ccAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCtt                                                                                              >  6:973325/1‑94 (MQ=255)
                                                                                        ccAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCtt                                                                                              <  5:973325/94‑1 (MQ=255)
                                                                                          aGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGGTGTAAAAGTTTTGAATAAAAGCTTACCCAAATAATATTTACACCTTTGTTAACACCCAAACGACTTGAAttg                                              >  5:247440/1‑140 (MQ=255)
                                                                                            ccGGCCCAGAGGTTTTCCGGTCGCGTCAACAAATTAACCGTCTAGTTTTCCG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTAATTATCATCGTAACCAATAAATAGTTACAAGTTGGTGAACACCCAATCGTCTTCAAttgtt                                            <  5:450514/139‑1 (MQ=255)
                                                                                            aCGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTC‑CTGGTTTAGCTGTAAAAGTTTTGATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAttgtt                                            >  1:919963/1‑140 (MQ=255)
                                                                                            aCGGCCCAGAGTTGTACCGGTCTCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATATTTACACGTTGGTGAACACCCAAACGTCTTCAAttg                                              <  3:174253/138‑1 (MQ=255)
                                                                                             cGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑c                                                                                                                               <  1:568135/56‑1 (MQ=255)
                                                                                             cGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑c                                                                                                                               >  2:568135/1‑56 (MQ=255)
                                                                                               gCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGATGGTGAACACCCAAACGTCTTCAATTGTTGAg                                         <  8:290852/140‑1 (MQ=255)
                                                                                                 ccAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑ATGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTAACCAATAAATAGTTTCACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAg                                         >  4:429404/1‑138 (MQ=255)
                                                                                                   agagGTGTACCGGTCGCGTCAACAACATACCAGTCGCGTTGTACG‑GTTT‑CTTGGTTAGCTGTAAATGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACTTGGGTTAACACCCAAACGTCTTCAATTGTTGAGGTTc                                     <  8:1089703/140‑1 (MQ=255)
                                                                                                    gagTTTTACCTGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGtt                                      <  4:953219/138‑1 (MQ=255)
                                                                                                    gagTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑TTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTGca                                    <  8:794401/140‑1 (MQ=255)
                                                                                                      gTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTcaca                                  <  7:372778/140‑1 (MQ=255)
                                                                                                          tACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACG‑GTTT‑CTGGTTTAGCTTTAAAATTTTTCATTAAAAGCTTACCCAATAAAAAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGTGTCACACgcc                              >  6:653379/1‑138 (MQ=255)
                                                                                                          tACCGGTCGCGTCAACAACATACCAGACGCGGTTTTAT‑GTTT‑GTGGTTTATCTGTTAAAGTTTTAAATAAAAGCAAACACAAAAAATATTTACACGTTGGTGAACACCCAAACGACTTGAAATTTTGAGGTTACCACgcc                              >  7:539119/1‑138 (MQ=255)
                                                                                                           aCCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACa                             <  5:264243/140‑1 (MQ=255)
                                                                                                                  gcggaaacaacATACCAGTCGCGTTTTAAG‑GTTT‑ATGGGTTAGCTGTAAAAGTTTTC‑TTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCgg                     <  6:1116823/135‑1 (MQ=255)
                                                                                                                   cgTCAACAACATAGCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGGTTTAATAGTTTTCATTAATAGCTTACCCAATAAATATTTACACGTTGGTGAACACCCAAACGTCTTCATTTGTTGAGGTTCACACGAGAAAGTCCgg                     <  6:297489/140‑1 (MQ=255)
                                                                                                                   cgTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCgg                     <  6:1031803/140‑1 (MQ=255)
                                                                                                                   cgTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTCGAGGTTCACACGACAAAGTCCgg                     <  8:937404/140‑1 (MQ=255)
                                                                                                                    gTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGc                    <  5:838113/140‑1 (MQ=255)
                                                                                                                           acaTACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCAGCAAACCTa             <  2:331881/140‑1 (MQ=255)
                                                                                                                                    tCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTACGGCAAACCTACCCCTTCGa    >  7:1169709/1‑140 (MQ=255)
                                                                                                                                     cgcgTTTTACT‑GTTT‑CTGGTTTAGCTTTAAATGTTTACATTAATAGGTTACCCAATAAATAGTTACACGTTGGTGAACACCCTAACGTCTTCATTTGTTGAGGTTCACACGTCAAAGTCCGGCAATCCTACCCCTTCGaa   <  7:1220274/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAGTAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  7:680755/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTTAACACCCAAACTTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  1:1169802/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCg     <  3:923240/138‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCg     <  3:1152032/138‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCg     <  3:1077686/138‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  5:532200/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  7:911223/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  5:1144785/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  7:618235/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  2:199539/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  8:729606/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  1:977411/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GTGT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  6:577554/140‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GGTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCg     <  4:718430/138‑1 (MQ=255)
                                                                                                                                     cgcgTTTTACG‑GGTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGGTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCg     <  4:850291/138‑1 (MQ=255)
                                                                                                                                     cgcgTGTTACG‑GTTT‑CTGGATTAGCTGTAAAAGTTTTGATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACTTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa   <  1:594745/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TTCAGAACGATGATGTAATCACCGGTATCTACGTGCGGAGTGTATTCCGCTTTGTGCTTACCGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACG‑GTTT‑CTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAAT  >  NZ_CP022686/2110320‑2110593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A