breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 45 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,663,7650TA64.3% 0.3 / 12.0 14I29F (ATT→TTT) ynfLLysR family transcriptional regulator
*NZ_CP0092731,906,0070TG63.6% 15.6 / 23.0 22H276P (CAC→CCC) htpXprotease HtpX
*NZ_CP0092732,738,4480AC59.3% 18.9 / 30.3 27V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
*NZ_CP0092731,906,0110TG59.1% 10.4 / 24.3 22T275P (ACC→CCC) htpXprotease HtpX
*NZ_CP0092733,578,4460AC59.1% 3.4 / 24.0 22Y580D (TAC→GAC) ggtgamma‑glutamyltransferase
*NZ_CP0092733,703,3320AC55.6% 12.5 / 22.2 18G168G (GGT→GGGeptBkdo(2)‑lipid A phosphoethanolamine 7''‑transferase
*NZ_CP009273353,5860AT54.5% ‑4.4 / 11.4 11N272K (AAT→AAAcynRtranscriptional regulator CynR
*NZ_CP009273510,0800AC54.5% 8.1 / 11.4 11V79G (GTG→GGG) ybbJNfeD family protein
*NZ_CP00927369,9000AC54.2% 12.1 / 22.4 24V370G (GTG→GGG) thiPthiamine/thiamine pyrophosphate ABC transporter permease ThiP
*NZ_CP009273489,9810AG53.8% 7.8 / 13.8 13M41V (ATG→GTG) recRrecombination mediator RecR
*NZ_CP0092732,388,9230AC53.3% 30.5 / 36.5 30G151G (GGT→GGGnuoJNADH‑quinone oxidoreductase subunit J
*NZ_CP0092734,594,1610CT53.1% 22.8 / 68.7 49S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092731,429,9750GA52.9% 7.7 / 22.3 17intergenic (‑99/+42)uspF/ompNuniversal stress protein UspF/porin OmpN
*NZ_CP009273822,9550TG52.6% 17.8 / 25.3 19T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
*NZ_CP0092731,598,4940CT52.6% 9.4 / 26.6 19S64L (TCG→TTG) lsrDautoinducer 2 ABC transporter permease LsrD
*NZ_CP0092734,123,1600AG52.4% 22.8 / 27.2 21E206G (GAA→GGA) metFmethylenetetrahydrofolate reductase
*NZ_CP0092733,357,7550AT52.2% 8.7 / 27.3 23P530P (CCA→CCTyhcDfimbrial biogenesis outer membrane usher protein
*NZ_CP0092733,175,9570AT51.8% 13.9 / 32.3 27I17F (ATT→TTT) zupTzinc transporter ZupT
*NZ_CP0092732,134,4070AC51.7% 30.6 / 44.9 29G229G (GGT→GGGasmAouter membrane assembly protein AsmA
*NZ_CP0092731,429,9840GA50.0% 7.5 / 12.2 18intergenic (‑108/+33)uspF/ompNuniversal stress protein UspF/porin OmpN

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 2772304 =29 (0.910)6 (0.190) 6/166 1.8 17.1% coding (3782/4581 nt) ypjA adhesin‑like autotransporter YpjA/EhaD
?NZ_CP009273 = 2991050 NA (NA)intergenic (‑91/+1) BW25113_RS14875/BW25113_RS14890 IS3‑like element IS2 family transposase/glycoside hydrolase family 15 protein
* ? NZ_CP009273 = 2283591NA (NA)8 (0.250) 5/164 2.0 18.4% intergenic (‑67/‑388) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
?NZ_CP009273 = 3512441 36 (1.130)intergenic (‑18/+383) aroK/hofQ shikimate kinase AroK/DNA uptake porin HofQ
* ? NZ_CP009273 1203246 =23 (0.720)3 (0.120) 3/134 2.3 13.2% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 21 (0.820)coding (40/540 nt) BW25113_RS06005 phage tail protein