breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 46 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,594,1610CT67.7% 1.1 / 25.1 32S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092731,152,7270TG62.5% 8.9 / 15.7 16G165G (GGT→GGGycfHmetal‑dependent hydrolase
*NZ_CP0092734,594,1670CT62.5% ‑4.2 / 30.6 32S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP009273886,7400CG61.9% 15.2 / 26.0 21A34G (GCC→GGC) nfsAnitroreductase NfsA
*NZ_CP0092733,594,9200AC61.1% 9.6 / 12.3 18V176G (GTG→GGG) ftsXpermease‑like cell division protein FtsX
*NZ_CP009273765,6750AC60.9% 17.2 / 22.5 23T748P (ACC→CCC) mngBmannosylglycerate hydrolase
*NZ_CP0092734,248,9060GT60.0% 11.7 / 22.9 15A395S (GCG→TCG) dinFMATE family efflux transporter DinF
*NZ_CP0092736,9940AC58.3% 13.6 / 24.9 24G322G (GGT→GGGyaaJsodium:alanine symporter family protein
*NZ_CP009273353,5910TC58.1% 21.4 / 40.6 31K271E (AAA→GAA) cynRtranscriptional regulator CynR
*NZ_CP009273497,0280AC57.1% 10.8 / 16.7 14V556G (GTG→GGG) ybaLKef family K(+) transporter
*NZ_CP009273497,0310AC57.1% 8.0 / 15.5 14V555G (GTG→GGG) ybaLKef family K(+) transporter
*NZ_CP0092734,248,9020TG57.1% 7.9 / 13.8 14G393G (GGT→GGGdinFMATE family efflux transporter DinF
*NZ_CP0092733,659,0620CA56.7% 18.3 / 51.1 30A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
*NZ_CP0092732,216,1540TG56.2% 8.0 / 14.5 16V164G (GTG→GGG) dldD‑lactate dehydrogenase
*NZ_CP0092732,134,4070AC55.0% 17.8 / 27.9 20G229G (GGT→GGGasmAouter membrane assembly protein AsmA
*NZ_CP009273353,6010CA54.3% 2.0 / 38.2 35L267F (TTG→TTTcynRtranscriptional regulator CynR
*NZ_CP0092733,023,3240AC53.8% 9.0 / 16.8 13L323V (TTA→GTA) ygfTformate‑dependent uric acid utilization protein YgfT
*NZ_CP009273106,2950TG53.3% 16.7 / 20.7 15G510G (GGT→GGGsecApreprotein translocase subunit SecA
*NZ_CP0092732,007,6930TC53.3% 9.3 / 14.0 16P328P (CCT→CCCfliFflagellar basal‑body MS‑ring/collar protein FliF
*NZ_CP0092734,102,5060TG53.3% 3.4 / 17.4 15G88G (GGT→GGGyiiRDUF805 domain‑containing protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 1203246 =31 (1.030)4 (0.160) 3/134 2.0 19.0% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 9 (0.370)coding (40/540 nt) BW25113_RS06005 phage tail protein