breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
JC | Annotation_U21_Trimmed_contig_1 | 47 | +84 bp | coding (47/567 nt) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 → | hypothetical protein |
RA | Annotation_U21_Trimmed_contig_4 | 75,126 | T→C | C31R (TGC→CGC) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 → | Replication‑associated recombination protein RarA |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | Annotation_U21_Trimmed_contig_1 | 1 = | 0 (0.000) | 185 (0.580) | 69/260 | 1.0 | 100% | coding (1/567 nt) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | hypothetical protein |
? | Annotation_U21_Trimmed_contig_8 | 1 = | 0 (0.000) | coding (588/588 nt) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | hypothetical protein | |||||
* | ? | Annotation_U21_Trimmed_contig_1 | = 38 | 84 (0.270) | 11 (0.580) +121 bp |
3/18 | 0.9 | 59.2% | coding (38/567 nt) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | hypothetical protein |
? | Annotation_U21_Trimmed_contig_1 | 76 = | 160 (0.520) | coding (76/567 nt) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | hypothetical protein | |||||
* | ? | Annotation_U21_Trimmed_contig_1 | = 50 | 103 (0.330) | 53 (1.110) +109 bp |
9/42 | 0.9 | 72.2% | coding (50/567 nt) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | hypothetical protein |
? | Annotation_U21_Trimmed_contig_1 | 76 = | 160 (0.520) | coding (76/567 nt) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | hypothetical protein | |||||
* | ? | Annotation_U21_Trimmed_contig_10 | 1 = | 0 (0.000) | 518 (0.470) +GTTTATTCCGGCA |
54/234 | 2.4 | 81.0% | noncoding (117/117 nt) | rasttk_feature_creation_tool=RNA_reps_5S_rRNA | 5S rRNA # 5S ribosomal RNA ‑ 3 prime truncation |
? | Annotation_U21_Trimmed_contig_12 | = 910 | 271 (0.800) | noncoding (8/73 nt) | rasttk_feature_creation_tool=tRNAscan‑SE | tRNA‑Lys‑TTT | |||||
* | ? | Annotation_U21_Trimmed_contig_10 | 1 = | 0 (0.000) | 409 (0.340) | 103/260 | 0.0 | 100% | noncoding (117/117 nt) | rasttk_feature_creation_tool=RNA_reps_5S_rRNA | 5S rRNA # 5S ribosomal RNA ‑ 3 prime truncation |
? | Annotation_U21_Trimmed_contig_12 | = 1004 | 0 (0.000) | intergenic (‑5/–) | rasttk_feature_creation_tool=tRNAscan‑SE/– | tRNA‑Val‑TAC/– | |||||
* | ? | Annotation_U21_Trimmed_contig_10 | 1 = | 0 (0.000) | 391 (0.490) +46 bp |
70/168 | 0.0 | 100% | noncoding (117/117 nt) | rasttk_feature_creation_tool=RNA_reps_5S_rRNA | 5S rRNA # 5S ribosomal RNA ‑ 3 prime truncation |
? | Annotation_U21_Trimmed_contig_3 | = 367983 | 0 (0.000) | intergenic (‑70/–) | rasttk_feature_creation_tool=tRNAscan‑SE/– | tRNA‑Thr‑GGT/– | |||||
* | ? | Annotation_U21_Trimmed_contig_10 | 1 = | 0 (0.000) | 360 (0.440) +46 bp |
71/168 | 0.1 | 100% | noncoding (117/117 nt) | rasttk_feature_creation_tool=RNA_reps_5S_rRNA | 5S rRNA # 5S ribosomal RNA ‑ 3 prime truncation |
? | Annotation_U21_Trimmed_contig_5 | = 139689 | 0 (0.000) | intergenic (+108/–) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2/– | Uncharacterized deacetylase/– | |||||
* | ? | Annotation_U21_Trimmed_contig_10 | = 3297 | 0 (0.000) | 1107 (0.570) +TTGAGAGTAA |
119/240 | 1.0 | 100% | intergenic (‑160/–) | rasttk_feature_creation_tool=RNA_reps_LSU_rRNA/– | LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/– |
? | Annotation_U21_Trimmed_contig_11 | = 1711 | 0 (0.000) | intergenic (+53/–) | rasttk_feature_creation_tool=RNA_reps_SSU_rRNA/– | SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/– | |||||
* | ? | Annotation_U21_Trimmed_contig_11 | 1 = | 0 (0.000) | 1980 (1.590) +C |
136/258 | 0.0 | 100.0% | intergenic (–/‑74) | –/rasttk_feature_creation_tool=RNA_reps_SSU_rRNA | –/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA |
? | Annotation_U21_Trimmed_contig_3 | 2 = | 1 (0.000) | intergenic (–/+370) | –/rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | –/Integral membrane protein LafC, accessory function in glycolipid and LTA synthesis | |||||
* | ? | Annotation_U21_Trimmed_contig_11 | = 1711 | 0 (0.000) | 505 (0.490) +22 bp |
78/216 | 0.0 | 77.3% | intergenic (+53/–) | rasttk_feature_creation_tool=RNA_reps_SSU_rRNA/– | SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/– |
? | Annotation_U21_Trimmed_contig_13 | 191 = | 358 (0.960) | noncoding (1/75 nt) | rasttk_feature_creation_tool=tRNAscan‑SE | tRNA‑Ile‑GAT | |||||
* | ? | Annotation_U21_Trimmed_contig_13 | 1 = | 0 (0.000) | 194 (0.550) | 50/260 | 2.0 | 56.1% | intergenic (–/‑4) | –/rasttk_feature_creation_tool=tRNAscan‑SE | –/tRNA‑Phe‑GAA |
? | Annotation_U21_Trimmed_contig_8 | 48167 = | 304 (0.920) | intergenic (‑22/+1) | rasttk_feature_creation_tool=tRNAscan‑SE/rasttk_feature_creation_tool=tRNAscan‑SE | tRNA‑Phe‑GAA/tRNA‑Asp‑GTC | |||||
* | ? | Annotation_U21_Trimmed_contig_2 | = 374547 | 476 (1.070) | 377 (0.840) | 72/256 | 1.7 | 61.7% | coding (3920/5343 nt) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | Predicted cell‑wall‑anchored protein SasA (LPXTG motif) |
? | Annotation_U21_Trimmed_contig_7 | = 60417 | 0 (0.000) | coding (3/753 nt) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | Predicted cell‑wall‑anchored protein SasA (LPXTG motif) | |||||
* | ? | Annotation_U21_Trimmed_contig_9 | 1 = | 0 (0.000) | 1111 (0.800) | 120/260 | 1.0 | 100% | coding (1/936 nt) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | Plasmid replication initiation protein |
? | Annotation_U21_Trimmed_contig_9 | = 10661 | NA (NA) | intergenic (+719/–) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2/– | ORF9/– |