breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG37.6% 447.2 / 62.7 258intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC36.5% 372.7 / 59.2 211G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306800,6750AC33.0% 529.9 / 56.7 274T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123063,020,6700TG32.8% 339.9 / 18.3 189K195N (AAA→AACglgCglucose‑1‑phosphate adenylyltransferase
*AP012306644,4170GA32.8% 876.2 / inf 627intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,309,9140AC32.7% 289.4 / 11.1 150L23F (TTA→TTClsrCAI2 transporter
*AP012306341,3770TG31.2% 218.9 / 27.0 125G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP012306257,1370TG28.8% 406.0 / 13.4 206L496V (TTG→GTG) betTcholine transporter of high affinity
*AP0123062,714,5550TG27.0% 624.5 / 21.5 273T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP0123063,527,3680AC26.4% 736.3 / 55.5 308L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123062,758,9090TG26.2% 565.6 / 23.0 233G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123061,066,3230AC25.8% 623.2 / 25.9 245intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
*AP012306202,4170TG24.6% 676.7 / 37.5 302V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP012306935,0540GT24.5% 264.4 / 49.6 98intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123063,938,8870CT24.4% 674.4 / 148.6 295S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123063,282,0760TG24.2% 637.5 / 25.1 286intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP0123063,903,7910AG23.0% 661.1 / 78.6 278E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123062,558,0710AC22.8% 645.9 / 38.9 265V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123061,226,5570AG22.8% 503.9 / 79.6 215G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123062,560,2430TG22.3% 525.4 / 18.0 212N419T (AAC→ACC) pepPproline aminopeptidase P II

Marginal new junction evidence (lowest skew 10 of 342 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 1092512398 (1.150)17 (0.060) 13/258 9.9 4.9% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 316 (1.050)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 997497321 (0.920)12 (0.040) 12/268 10.3 4.0% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 283 (0.910)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 3923390 =454 (1.310)10 (0.030) 10/288 11.2 2.2% coding (119/1032 nt) idnD L‑idonate 5‑dehydrogenase, NAD‑binding
?AP012306 3923413 = 447 (1.330)coding (96/1032 nt) idnD L‑idonate 5‑dehydrogenase, NAD‑binding
* ? AP012306 = 3600658NA (NA)33 (0.100) 10/292 11.2 NA noncoding (2805/2904 nt) rrlB 23S ribosomal RNA (rrlB)
?AP012306 = 3642045 NA (NA)noncoding (2790/2904 nt) rrlE 23S ribosomal RNA (rrlE)
* ? AP012306 = 3600650NA (NA)57 (0.170)
+AAAGG
10/288 11.2 NA noncoding (2797/2904 nt) rrlB 23S ribosomal RNA (rrlB)
?AP012306 = 3642048 NA (NA)noncoding (2793/2904 nt) rrlE 23S ribosomal RNA (rrlE)
* ? AP012306 = 215364NA (NA)10 (0.030) 10/288 11.2 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 3021636 =317 (0.910)9 (0.030) 9/290 11.5 2.8% coding (1610/1974 nt) glgX glycogen debranching enzyme
?AP012306 3021656 = 313 (0.930)coding (1590/1974 nt) glgX glycogen debranching enzyme
* ? AP012306 = 2094536262 (0.750)8 (0.030) 8/274 11.6 3.2% coding (394/834 nt) cysU sulfate/thiosulfate transporter subunit
?AP012306 = 2094524 241 (0.750)coding (406/834 nt) cysU sulfate/thiosulfate transporter subunit
* ? AP012306 = 96920334 (0.960)8 (0.030) 8/274 11.6 2.5% coding (1331/1476 nt) murC UDP‑N‑acetylmuramate:L‑alanine ligase
?AP012306 = 96908 305 (0.960)coding (1319/1476 nt) murC UDP‑N‑acetylmuramate:L‑alanine ligase
* ? AP012306 = 1092334304 (0.870)7 (0.020) 7/258 11.7 2.4% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 316 (1.050)noncoding (66/85 nt) tyrT tRNA‑Tyr