breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 464 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,725,7391.C74.9% 465.9 / 151.8 184coding (1262/1455 nt)xylBxylulokinase
*NC_0009133,778,4750AG74.6% 444.2 / 212.8 291Q341R (CAG→CGG) lldDL‑lactate dehydrogenase
*NC_000913283,3560AG74.4% 341.3 / 161.0 223N311S (AAT→AGT) yagFputative dehydratase
*NC_0009133,916,1680AG74.4% 526.1 / 259.0 347S440P (TCC→CCC) atpAmembrane‑bound ATP synthase, F1 sector, alpha‑subunit
*NC_000913718,7460TC74.2% 425.4 / 202.3 275Y313C (TAC→TGC) speFornithine decarboxylase isozyme
*NC_0009133,767,2700TC74.1% 119.7 / 57.6 81V100A (GTA→GCA) b4549hypothetical protein
*NC_0009131,846,5040GA74.0% 438.6 / 167.8 278A66V (GCG→GTG) ydjAhypothetical protein
*NC_0009133,824,4670TC73.9% 424.1 / 203.9 284L544P (CTA→CCA) recGDNA helicase
*NC_0009133,718,3500CT73.9% 596.9 / 196.4 303V14V (GTC→GTTyi5AIS150 hypothetical protein
*NC_0009134,344,6250AG73.7% 422.1 / 197.0 278S94P (TCC→CCC) fumBfumarase B
*NC_00091330,5720AG73.6% 448.1 / 208.3 299T308A (ACC→GCC) carAcarbamoyl‑phosphate synthetase
*NC_0009131,599,6330CT73.5% 476.6 / 161.1 249H40H (CAC→CATb1513putative ATP‑binding component of a transport system
*NC_000913580,6770CT73.4% 505.4 / 176.3 274A34V (GCG→GTG) ybcXhypothetical protein
*NC_000913385,9420TC73.3% 457.4 / 243.3 315L171P (CTG→CCG) tauBTauB
*NC_000913703,2940AG73.0% 330.0 / 175.3 233Q43R (CAG→CGG) nagEPTS system, N‑acetylglucosamine‑specific enzyme IIABC
*NC_000913705,0130CT72.9% 427.8 / 147.9 225P616L (CCG→CTG) nagEPTS system, N‑acetylglucosamine‑specific enzyme IIABC
*NC_0009131,917,0050TC72.7% 348.1 / 176.2 242M493T (ATG→ACG) b1834hypothetical protein
*NC_0009132,912,0950TC72.6% 367.8 / 205.2 263S310G (AGT→GGT) ygcAhypothetical protein
*NC_000913580,4310TA72.4% 21.6 / 24.8 29I125I (ATT→ATAnohBbacteriophage DNA packaging protein
*NC_0009133,648,5360CT72.1% 355.3 / 134.8 197intergenic (+244/‑385)arsC/yhiSarsenate reductase/hypothetical protein

Marginal new junction evidence (lowest skew 10 of 16 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3980297276 (0.940)146 (0.560)
+CCC
83/162 3.2 48.1% noncoding (79/87 nt) leuT tRNA‑Leu
?NC_000913 = 4603655 75 (0.280)noncoding (80/88 nt) leuV tRNA‑Leu
* ? NC_000913 = 460365563 (0.230)158 (0.550)
+GGG
80/162 3.7 51.1% noncoding (80/88 nt) leuV tRNA‑Leu
?NC_000913 4603892 = 243 (0.830)noncoding (79/87 nt) leuQ tRNA‑Leu
* ? NC_000913 = 460365562 (0.230)158 (0.550)
+GGG
79/162 3.8 71.4% noncoding (80/88 nt) leuV tRNA‑Leu
?NC_000913 4603656 = 63 (0.210)noncoding (79/88 nt) leuV tRNA‑Leu
* ? NC_000913 4540250 =94 (0.320)90 (0.340) 64/166 6.9 42.9% intergenic (+49/‑433) fimE/fimA FimE/major type 1 subunit fimbrin
?NC_000913 4540564 = 155 (0.580)intergenic (+363/‑119) fimE/fimA FimE/major type 1 subunit fimbrin
* ? NC_000913 = 3313058294 (1.000)50 (0.180) 45/174 12.4 15.2% coding (598/2673 nt) infB protein chain initiation factor IF‑2
?NC_000913 3313308 = 279 (1.000)coding (348/2673 nt) infB protein chain initiation factor IF‑2
* ? NC_000913 = 4540258108 (0.370)42 (0.160) 37/166 14.1 25.2% intergenic (+57/‑425) fimE/fimA FimE/major type 1 subunit fimbrin
?NC_000913 = 4540554 152 (0.570)intergenic (+353/‑129) fimE/fimA FimE/major type 1 subunit fimbrin
* ? NC_000913 = 4008398144 (0.500)156 (0.550)
+AAGA
31/172 16.8 52.8% coding (481/516 nt) b4555 hypothetical protein
?NC_000913 4008399 = 144 (0.490)coding (482/516 nt) b4555 hypothetical protein
* ? NC_000913 = 4603655154 (0.570)64 (0.220)
+GGG
23/162 19.2 22.7% noncoding (80/88 nt) leuV tRNA‑Leu
?NC_000913 4603777 = 282 (0.960)noncoding (79/87 nt) leuP tRNA‑Leu
* ? NC_000913 = 3980289243 (0.900)75 (0.260)
+CCC
14/162 23.9 23.0% noncoding (71/87 nt) leuT tRNA‑Leu
?NC_000913 3980290 = 253 (0.860)noncoding (72/87 nt) leuT tRNA‑Leu
* ? NC_000913 1409923 =305 (1.040)9 (0.040) 9/132 23.9 3.9% coding (50/936 nt) ydaO hypothetical protein
?NC_000913 = 1432982 220 (1.050)intergenic (‑701/+227) ynaE/ynaF hypothetical protein/putative filament protein