breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 375 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,949,3560TC74.2% 1477.0 / inf 983S178S (AGT→AGCyecDhypothetical protein
*NC_0009131,949,3590CT73.4% 1777.5 / inf 969I179I (ATC→ATTyecDhypothetical protein
*NC_0009131,949,3620CT72.8% 1677.5 / inf 956N180N (AAC→AATyecDhypothetical protein
*NC_0009131,949,4360AG72.1% 1137.8 / inf 825L6L (CTA→CTGyecEhypothetical protein
*NC_0009131,634,0230AT72.1% 54.4 / 59.4 68G123G (GGT→GGAnohANohA
*NC_0009131,949,4110AC71.8% 2032.8 / inf 943N197H (AAC→CAC) yecDhypothetical protein
*NC_0009131,949,4100CT71.3% 1624.4 / inf 949L196L (CTC→CTTyecDhypothetical protein
*NC_0009133,941,0900TC70.0% 175.7 / 199.5 237noncoding (34/76 nt)gltUtRNA‑Val
*NC_000913526,3020G.68.0% 351.0 / 312.8 247coding (3818/4281 nt)rhsDRhsD
*NC_0009133,847,8770GA67.0% 603.6 / inf 424A159A (GCC→GCTuhpAUhpA
*NC_0009134,117,3630GA66.9% 525.7 / inf 393C189C (TGC→TGTmenAMenA
*NC_0009134,284,1900TC66.6% 312.7 / 292.4 324N254S (AAC→AGC) acsacetyl‑CoA synthetase
*NC_0009133,951,0820CT66.5% 606.7 / inf 459intergenic (+46/‑50)ilvE/ilvDbranched‑chain amino‑acid aminotransferase/dihydroxyacid dehydratase
*NC_000913580,4980GA66.2% 51.9 / 59.9 77V148I (GTT→ATT) nohBbacteriophage DNA packaging protein
*NC_0009133,161,2240TC66.0% 321.7 / inf 371T92A (ACG→GCG) plsC1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
*NC_0009131,949,4720GT65.8% 420.8 / inf 492G18G (GGG→GGTyecEhypothetical protein
*NC_0009133,376,0380AG65.6% 390.8 / inf 410G83G (GGT→GGCrplM50S ribosomal subunit protein L13
*NC_0009134,020,0450CT65.0% 475.8 / inf 411A54V (GCG→GTG) b3838hypothetical protein
*NC_0009133,436,8370TC64.9% 443.0 / inf 527T104A (ACC→GCC) yhdNhypothetical protein
*NC_0009133,817,9670AG64.9% 342.5 / inf 424D279D (GAT→GACyicFhypothetical protein

Marginal new junction evidence (lowest skew 10 of 21 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1986123246 (0.680)72 (0.190)
+CCC
35/166 5.7 22.3% intergenic (+318/‑122) b1903/b1904 hypothetical protein/hypothetical protein
?NC_000913 1986124 = 256 (0.660)intergenic (+319/‑121) b1903/b1904 hypothetical protein/hypothetical protein
* ? NC_000913 3422995 =NA (NA)74 (0.200) 34/178 6.2 NA noncoding (1426/2904 nt) rrlD 23S ribosomal RNA
?NC_000913 3942750 = NA (NA)noncoding (1425/2906 nt) rrlC 23S ribosomal RNA
* ? NC_000913 = 2725778NA (NA)217 (0.580)
+CCC
29/166 6.5 100% noncoding (1427/2904 nt) rrlG 23S ribosomal RNA
?NC_000913 2725779 = 0 (0.000)noncoding (1426/2904 nt) rrlG 23S ribosomal RNA
* ? NC_000913 = 4008398125 (0.330)86 (0.230)
+AAGA
29/172 6.7 41.6% coding (481/516 nt) b4555 hypothetical protein
?NC_000913 4008399 = 125 (0.320)coding (482/516 nt) b4555 hypothetical protein
* ? NC_000913 = 227178NA (NA)210 (0.560)
+GGG
26/166 7.0 NA noncoding (1420/2904 nt) rrlH 23S ribosomal RNA
?NC_000913 227179 = NA (NA)noncoding (1421/2904 nt) rrlH 23S ribosomal RNA
* ? NC_000913 = 34244400 (0.000)1417 (4.500)
+TTTCGATTCATCATCGT
6/150 11.3 100% intergenic (‑20/+155) rrlD/alaU 23S ribosomal RNA/tRNA‑Asx
?NC_000913 3424449 = NA (NA)intergenic (‑29/+146) rrlD/alaU 23S ribosomal RNA/tRNA‑Asx
* ? NC_000913 = 2727224NA (NA)745 (2.370)
+TTTCGATTCATCATCGT
5/150 11.8 71.9% intergenic (‑20/+165) rrlG/gltW 23S ribosomal RNA/tRNA‑Sec
?NC_000913 2727233 = 358 (0.930)intergenic (‑29/+156) rrlG/gltW 23S ribosomal RNA/tRNA‑Sec
* ? NC_000913 = 274135NA (NA)6 (0.020) 6/180 12.1 NA coding (207/1017 nt) trs51 IS5 transposase
?NC_000913 = 274151 NA (NA)coding (191/1017 nt) trs51 IS5 transposase
* ? NC_000913 = 3129355NA (NA)36 (0.090) 6/184 12.2 14.6% intergenic (+146/+1) trs59/b2983 IS5 transposase/hypothetical protein
?NC_000913 3132097 = 211 (0.550)intergenic (‑125/‑49) yghS/yghT hypothetical protein/hypothetical protein
* ? NC_000913 3431823 =584 (1.510)5 (0.010) 4/160 12.5 0.97% coding (497/510 nt) def peptide deformylase
?NC_000913 3431835 = 509 (1.520)coding (509/510 nt) def peptide deformylase