breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 209 shown, sorted by frequency from high to low)
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,747,903	0	G	T	56.4%	‑1.4 / inf	484	A8S (GCA→TCA)	ydiL	hypothetical protein
*	REL606	720,466	0	G	C	53.8%	7.0 / 32.1	27	A23P (GCC→CCC)	phrB	deoxyribodipyrimidine photolyase, FAD‑binding
*	REL606	2,437,406	0	G	T	52.5%	24.9 / 71.0	40	D435E (GAC→GAA)	ypdD	fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*	REL606	433	0	T	G	52.4%	55.8 / 120.0	82	V33G (GTG→GGG)	thrA	bifunctional aspartokinase I/homeserine dehydrogenase I
*	REL606	895,856	0	G	C	50.0%	13.8 / 48.9	40	A26P (GCC→CCC)	ECB_00836	putative Regulatory protein
*	REL606	2,515,909	0	A	C	48.6%	69.9 / 43.2	74	V260G (GTG→GGG)	ypfI	predicted hydrolase
*	REL606	1,854,401	0	A	C	48.2%	61.9 / 62.0	83	K88N (AAA→AAC)	yeaO	hypothetical protein
*	REL606	931,314	0	T	C	44.7%	80.9 / 62.7	103	E84G (GAG→GGG)	ybjE	predicted transporter
*	REL606	720,464	0	C	G	42.4%	26.4 / 33.1	33	A22G (GCC→GGC)	phrB	deoxyribodipyrimidine photolyase, FAD‑binding
*	REL606	4,180,249	0	T	G	40.8%	227.4 / 55.7	147	G24G (GGT→GGG)	yjaH	hypothetical protein
*	REL606	1,853,067	0	A	C	37.7%	261.2 / 116.6	175	T56P (ACC→CCC)	yeaN	predicted transporter
*	REL606	3,774,914	0	C	G	37.1%	341.9 / 193.1	221	A20P (GCC→CCC)	yicJ	predicted transporter
*	REL606	254,043	0	T	G	37.0%	102.3 / 25.5	73	pseudogene (588/1710 nt)	fhiA	b0229; flagellar system protein
*	REL606	3,472,700	0	C	G	36.8%	125.2 / 83.9	106	A290G (GCG→GGG)	yhgA	predicted transposase
*	REL606	3,202,996	0	A	C	36.3%	401.3 / 16.4	212	intergenic (+444/+558)	yhaC/garK	hypothetical protein/glycerate kinase I
*	REL606	2,535,009	0	A	C	36.3%	288.1 / 24.4	146	T86P (ACC→CCC)	focB	predicted formate transporter
*	REL606	3,344,466	0	T	G	36.1%	499.0 / 108.6	263	F515V (TTT→GTT)	acrF	multidrug efflux system protein
*	REL606	2,376,348	0	T	G	36.0%	306.1 / 56.5	179	T63P (ACC→CCC)	folC	bifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*	REL606	4,392,556	0	C	T	35.9%	245.1 / 113.0	181	L43L (CTA→TTA)	aidB	isovaleryl CoA dehydrogenase
*	REL606	159,654	0	A	C	35.5%	537.8 / 46.0	262	G27G (GGT→GGG)	yadN	predicted fimbrial‑like adhesin protein

Marginal new junction evidence (sorted from low to high skew)
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	1699810 =	319 (1.180)	5 (0.020)	5/128	18.6	1.8%	coding (496/2013 nt)	ydhK	conserved inner membrane protein
	?	REL606	= 1699806	283 (1.230)					coding (492/2013 nt)	ydhK	conserved inner membrane protein
*	?	REL606	= 379585	407 (1.510)	197 (0.730)	6/150	19.4	28.9%	intergenic (+110/+135)	yajF/araJ	fructokinase/predicted transporter
	?	REL606	= 2583282	562 (2.080)					coding (741/741 nt)	yfhQ	predicted methyltransferase
*	?	REL606	2066317 =	287 (1.060)	3 (0.010)	3/128	20.2	1.2%	coding (287/840 nt)	wcaA	predicted glycosyl transferase
	?	REL606	= 2066313	243 (1.060)					coding (291/840 nt)	wcaA	predicted glycosyl transferase
*	?	REL606	2781178 =	309 (1.140)	3 (0.010)	3/132	20.4	1.1%	coding (22/1272 nt)	ygcN	predicted oxidoreductase with FAD/NAD(P)‑binding domain
	?	REL606	= 2781174	276 (1.160)					coding (18/1272 nt)	ygcN	predicted oxidoreductase with FAD/NAD(P)‑binding domain