breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 430,954 | G→A | E4E (GAG→GAA) | lon → | DNA‑binding ATP‑dependent protease La |
RA | 475,031 | G→T | A92E (GCG→GAG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
RA | 649,985 | C→G | V273L (GTG→CTG) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,028,170 | G→A | R98* (CGA→TGA) | ECB_02828 ← | Polysialic acid transport protein kpsM |
RA | 3,339,489 | Δ1 bp | intergenic (+31/‑55) | fis → / → yhdJ | DNA‑binding protein Fis/predicted methyltransferase |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 3,911,991 | (T)5→4 | intergenic (‑192/‑131) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
RA | 4,160,666 | T→G | intergenic (+130/‑190) | rplL → / → rpoB | 50S ribosomal protein L7/L12/DNA‑directed RNA polymerase subunit beta |
RA | 4,161,978 | C→T | P375S (CCG→TCG) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,504,637 | G→A | intergenic (+381/‑344) | insB‑25 → / → ECB_04162 | b4563(b4576); IS1 protein insB/hypothetical protein |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 2651895 | NA (NA) | 39 (0.850) | 30/144 | 0.5 | NA | noncoding (670/1542 nt) | rrsG | 16S ribosomal RNA |
? | REL606 | = 2774196 | NA (NA) | noncoding (1343/1343 nt) | IS186 | repeat region |