breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,004,1060AC42.1% 71.0 / 26.4 57intergenic (‑32/+570)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
*REL6063,492,0820TG40.3% 91.8 / 29.7 62V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6064,393,0610TG32.8% 112.3 / 26.1 58V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6063,691,8440TG32.7% 101.6 / 18.1 55N226T (AAC→ACC) yiaYpredicted Fe‑containing alcohol dehydrogenase
*REL606154,4970TG31.6% 124.3 / 10.8 57A191A (GCA→GCCyadLpredicted fimbrial‑like adhesin protein
*REL6062,296,3290AC31.6% 118.0 / 20.7 57intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6061,688,9900AC30.3% 178.7 / 14.6 76T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*REL6061,882,5260TG27.9% 141.7 / 12.9 79I21S (ATT→AGT) manZmannose‑specific enzyme IID component of PTS
*REL6064,043,4260TA25.0% 132.2 / 13.5 56I200F (ATT→TTT) yihOpredited transporter
*REL6063,733,7190CG22.9% 125.0 / 14.1 49G63G (GGC→GGGgumDADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding
*REL6061,484,1050AG22.2% 152.3 / 13.9 63G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6063,983,7000TG20.8% 125.1 / 11.8 48T397P (ACC→CCC) ECB_03713conserved hypothetical protein

Marginal new junction evidence (lowest skew 10 of 28 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2038074 =43 (0.640)8 (0.130) 7/132 6.5 15.3% coding (57/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 2038286 = 50 (0.840)coding (663/822 nt) wbbB putative glycosyltransferase WbbB
* ? REL606 = 203739234 (0.510)5 (0.080) 5/134 7.6 14.8% coding (739/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 = 2037686 27 (0.450)coding (445/1176 nt) wzy O antigen polymerase (Wzy)
* ? REL606 3021448 =51 (0.760)5 (0.080) 5/138 7.8 8.2% coding (36/933 nt) ECB_02820 hypothetical protein
?REL606 3021652 = 65 (1.050)coding (1033/1140 nt) ECB_02821 hypothetical protein
* ? REL606 = 293660060 (0.900)4 (0.070) 4/132 8.1 8.0% intergenic (‑181/+266) gcvT/visC glycine cleavage system aminomethyltransferase T/hypothetical protein
?REL606 = 2936862 39 (0.660)intergenic (‑443/+4) gcvT/visC glycine cleavage system aminomethyltransferase T/hypothetical protein
* ? REL606 2035404 =55 (0.820)4 (0.070) 4/132 8.1 7.1% coding (453/768 nt) wbbD putative glycosyltransferase WbbD
?REL606 2035675 = 55 (0.930)coding (182/768 nt) wbbD putative glycosyltransferase WbbD
* ? REL606 = 203736327 (0.400)4 (0.070) 4/134 8.2 13.0% coding (768/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 = 2037670 29 (0.480)coding (461/1176 nt) wzy O antigen polymerase (Wzy)
* ? REL606 = 204025359 (0.880)4 (0.070) 4/136 8.3 8.9% intergenic (‑125/+18) vioB/vioA VioB, involved in dTDP‑N‑acetylviosamine synthesis/VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 = 2040442 28 (0.460)coding (942/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
* ? REL606 = 358233664 (0.960)5 (0.080) 4/138 8.4 7.4% coding (356/1224 nt) yhiS hypothetical protein
?REL606 = 3582485 66 (1.060)coding (505/1224 nt) yhiS hypothetical protein
* ? REL606 = 302154745 (0.670)4 (0.070) 3/124 8.4 9.9% coding (1138/1140 nt) ECB_02821 hypothetical protein
?REL606 = 3021727 35 (0.630)coding (958/1140 nt) ECB_02821 hypothetical protein
* ? REL606 = 204027254 (0.810)5 (0.080) 4/138 8.4 10.6% coding (1112/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 = 2040459 34 (0.550)coding (925/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis