breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 1,004,106 | 0 | A | C | 42.1% | 71.0 / 26.4 | 57 | intergenic (‑32/+570) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
* | REL606 | 3,492,082 | 0 | T | G | 40.3% | 91.8 / 29.7 | 62 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 4,393,061 | 0 | T | G | 32.8% | 112.3 / 26.1 | 58 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 3,691,844 | 0 | T | G | 32.7% | 101.6 / 18.1 | 55 | N226T (AAC→ACC) | yiaY | predicted Fe‑containing alcohol dehydrogenase |
* | REL606 | 154,497 | 0 | T | G | 31.6% | 124.3 / 10.8 | 57 | A191A (GCA→GCC) | yadL | predicted fimbrial‑like adhesin protein |
* | REL606 | 2,296,329 | 0 | A | C | 31.6% | 118.0 / 20.7 | 57 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 1,688,990 | 0 | A | C | 30.3% | 178.7 / 14.6 | 76 | T92P (ACC→CCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) |
* | REL606 | 1,882,526 | 0 | T | G | 27.9% | 141.7 / 12.9 | 79 | I21S (ATT→AGT) | manZ | mannose‑specific enzyme IID component of PTS |
* | REL606 | 4,043,426 | 0 | T | A | 25.0% | 132.2 / 13.5 | 56 | I200F (ATT→TTT) | yihO | predited transporter |
* | REL606 | 3,733,719 | 0 | C | G | 22.9% | 125.0 / 14.1 | 49 | G63G (GGC→GGG) | gumD | ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding |
* | REL606 | 1,484,105 | 0 | A | G | 22.2% | 152.3 / 13.9 | 63 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | REL606 | 3,983,700 | 0 | T | G | 20.8% | 125.1 / 11.8 | 48 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
Marginal new junction evidence (lowest skew 10 of 28 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 2038074 = | 43 (0.640) | 8 (0.130) | 7/132 | 6.5 | 15.3% | coding (57/1176 nt) | wzy | O antigen polymerase (Wzy) |
? | REL606 | 2038286 = | 50 (0.840) | coding (663/822 nt) | wbbB | putative glycosyltransferase WbbB | |||||
* | ? | REL606 | = 2037392 | 34 (0.510) | 5 (0.080) | 5/134 | 7.6 | 14.8% | coding (739/1176 nt) | wzy | O antigen polymerase (Wzy) |
? | REL606 | = 2037686 | 27 (0.450) | coding (445/1176 nt) | wzy | O antigen polymerase (Wzy) | |||||
* | ? | REL606 | 3021448 = | 51 (0.760) | 5 (0.080) | 5/138 | 7.8 | 8.2% | coding (36/933 nt) | ECB_02820 | hypothetical protein |
? | REL606 | 3021652 = | 65 (1.050) | coding (1033/1140 nt) | ECB_02821 | hypothetical protein | |||||
* | ? | REL606 | = 2936600 | 60 (0.900) | 4 (0.070) | 4/132 | 8.1 | 8.0% | intergenic (‑181/+266) | gcvT/visC | glycine cleavage system aminomethyltransferase T/hypothetical protein |
? | REL606 | = 2936862 | 39 (0.660) | intergenic (‑443/+4) | gcvT/visC | glycine cleavage system aminomethyltransferase T/hypothetical protein | |||||
* | ? | REL606 | 2035404 = | 55 (0.820) | 4 (0.070) | 4/132 | 8.1 | 7.1% | coding (453/768 nt) | wbbD | putative glycosyltransferase WbbD |
? | REL606 | 2035675 = | 55 (0.930) | coding (182/768 nt) | wbbD | putative glycosyltransferase WbbD | |||||
* | ? | REL606 | = 2037363 | 27 (0.400) | 4 (0.070) | 4/134 | 8.2 | 13.0% | coding (768/1176 nt) | wzy | O antigen polymerase (Wzy) |
? | REL606 | = 2037670 | 29 (0.480) | coding (461/1176 nt) | wzy | O antigen polymerase (Wzy) | |||||
* | ? | REL606 | = 2040253 | 59 (0.880) | 4 (0.070) | 4/136 | 8.3 | 8.9% | intergenic (‑125/+18) | vioB/vioA | VioB, involved in dTDP‑N‑acetylviosamine synthesis/VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | = 2040442 | 28 (0.460) | coding (942/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis | |||||
* | ? | REL606 | = 3582336 | 64 (0.960) | 5 (0.080) | 4/138 | 8.4 | 7.4% | coding (356/1224 nt) | yhiS | hypothetical protein |
? | REL606 | = 3582485 | 66 (1.060) | coding (505/1224 nt) | yhiS | hypothetical protein | |||||
* | ? | REL606 | = 3021547 | 45 (0.670) | 4 (0.070) | 3/124 | 8.4 | 9.9% | coding (1138/1140 nt) | ECB_02821 | hypothetical protein |
? | REL606 | = 3021727 | 35 (0.630) | coding (958/1140 nt) | ECB_02821 | hypothetical protein | |||||
* | ? | REL606 | = 2040272 | 54 (0.810) | 5 (0.080) | 4/138 | 8.4 | 10.6% | coding (1112/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | = 2040459 | 34 (0.550) | coding (925/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |