mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 474,424 | A→T | D294E (GAT→GAA) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| RA | 648,716 | A→T | V61E (GTG→GAG) | mrdB ← | cell wall shape‑determining protein |
| RA | 1,262,127 | C→T | intergenic (‑102/+49) | prsA ← / ← ispE | ribose‑phosphate pyrophosphokinase/4‑diphosphocytidyl‑2‑C‑methyl‑D‑erythritol kinase |
| RA | 1,290,440 | Δ1 bp | coding (405/1014 nt) | hnr → | response regulator of RpoS |
| MC JC | 1,304,475 | Δ620 bp | [yciU]–[cls] | [yciU], [cls] | |
| RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
| JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
| RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| RA | 2,298,651 | Δ1 bp | coding (432/1359 nt) | glpT ← | sn‑glycerol‑3‑phosphate transporter |
| RA | 2,749,029 | G→A | V292M (GTG→ATG) | hypE → | carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein |
| RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
| RA | 4,164,484 | T→A | I1210N (ATC→AAC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | REL606 | 546995–547700 | 558812 | 11113–11818 | 15 [13] | [14] 15 | [insB‑6]–ECB_00517 | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517 |
| * | * | ÷ | REL606 | 559074 | 573841 | 14768 | 15 [13] | [14] 15 | [appY]–ECB_00530 | [appY],ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530 |
| * | * | ÷ | REL606 | 574050 | 593363 | 19314 | 15 [14] | [14] 15 | cusS–[fepA] | cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
| * | * | ÷ | REL606 | 593577 | 619827–619116 | 25540–26251 | 15 [14] | [14] 16 | [fepA]–[insA‑8] | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8] |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 2038724 = | 117 (2.070) | 28 (0.560) | 14/134 | 2.6 | 21.9% | coding (225/822 nt) | wbbB | putative glycosyltransferase WbbB |
| ? | REL606 | 2039214 = | 96 (1.900) | coding (481/747 nt) | wbbA | putative glycosyltransferase WbbA | |||||
| * | ? | REL606 | 2041054 = | 106 (1.880) | 56 (1.090) | 34/136 | 0.5 | 28.1% | coding (330/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
| ? | REL606 | 2041468 = | 191 (3.730) | coding (1353/1428 nt) | wzx | O antigen translocase (Wzx) | |||||