breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA BX571857 135,427 C→T 70.2% R134* (CGA→TGA)  SAS0118 → putative exported protein
RA BX571857 378,366 T→G 6.3% I288S (ATT→AGT)  SAS0330 → acetyl‑CoA acetyltransferase
RA BX571857 549,806 A→G 70.5% intergenic (‑141/‑17) SAS0478 ← / → SAS0479 nucleoside permease/putative DNA‑binding protein
RA BX571857 621,623 G→A 69.4% A42A (GCG→GCA SAS0539 → putative uracil‑DNA glycosylase
RA BX571857 627,445 A→T 13.6% intergenic (‑133/‑35) SAS0546 ← / → SAS0547 conserved hypothetical protein/putative phosphate acetyltransferase
JC BX571857 1,034,532 Δ4 bp 17.1% coding (451‑454/603 nt) SAS0962 → putative membrane protein
RA BX571857 1,059,249 A→T 6.2% S429T (TCG→ACG)  SAS0988 ← bifunctional autolysin precursor
RA BX571857 1,252,438 T→C 100% intergenic (+358/‑77) SAS1171 → / → SAS1172 signal recognition particle protein/30S ribosomal protein S16
RA BX571857 1,380,051 A→C 14.6% D73A (GAT→GCT)  SAS1289 → aconitate hydratase
RA BX571857 1,434,357 C→G 12.0% A141G (GCT→GGT)  SAS1338 → putative tetrahydrodipicolinate acetyltransferase
RA BX571857 1,868,342 G→A 100% G129D (GGT→GAT)  SAS1720 → putative membrane protein
RA BX571857 2,379,210 G→T 9.5% A51E (GCA→GAA)  SAS2224 ← urocanate hydratase
RA BX571857 2,602,946 G→T 77.3% G116G (GGC→GGA SAS2426 ← putative membrane protein
RA BX571857 2,700,137 A→G 5.1% D777D (GAT→GAC SAS2516 ← fibrinogen and keratin‑10 binding surface anchored protein
RA BX571857 2,700,425 A→G 7.7% D681D (GAT→GAC SAS2516 ← fibrinogen and keratin‑10 binding surface anchored protein
RA BX571858 20,628:1 +G 100% coding (288/306 nt) pSAS24 → putative exported protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ BX571858 20652 20652 1 442 [0] [0] NA pSAS24/– putative exported protein/–

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? BX571857 980997 =NA (NA)17 (0.120) 16/118 NT NA noncoding (1/27 nt) Repeat flanking prophage phiSa4(476) Repeat flanking prophage phiSa4(476)
?BX571857 = 1025750 NA (NA)intergenic (+519/‑57) SAS0954/SAS0955 amidase/putative protease
* ? BX571858 1 =0 (0.000)384 (1.110) 130/170 NT 100% intergenic (–/‑11) –/pSAS01 –/putative ABC transporter ATP binding protein
?BX571858 = 20651 0 (0.000)intergenic (+5/–) pSAS24/– putative exported protein/–