breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 444,779 C→T W440* (TGG→TAG)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 1,191,676 C→T H366H (CAC→CAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,688 C→T T370T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,701 T→C L375M (TTA→CTG)  icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,703 A→G L375M (TTA→CTG icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 2,305,946 G→T R95S (CGT→AGT)  ompC ← outer membrane porin protein C
RA 2,506,454 C→T intergenic (‑269/‑67) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter
MC JC 3,341,548 Δ17,367 bp IS5‑mediated [npr]yhcE [npr], yrbL, mtgA, elbB, arcB, yhcC, gltB, gltD, gltF, yhcA, yhcD, yhcE
RA 3,528,506 A→C V241G (GTA→GGA)  envZ ← sensory histidine kinase in two‑component regulatory system with OmpR
RA 3,676,832 G→T intergenic (‑25/+158) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
JC 3,676,860 (GGTACCTGCGG)1→2 intergenic (‑53/+130) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
RA 4,568,602 C→A E185* (GAG→TAG)  mcrB ← 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 1116995 1118105 1111 26 [25] [24] 26 pyrC pyrC
* * ÷ CP009273 1191733 1206923 15191 26 [21] [25] 26 [icd]–[icdC] 25 genes
[icd], ymfD, ymfE, lit, intE, xisE, ymfI, ymfJ, cohE, croE, ymfL, ymfM, oweE, aaaE, ymfR, beeE, jayE, ymfQ, stfP, tfaP, tfaE, stfE, pinE, mcrA, [icdC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 1045234 =57 (0.930)57 (0.930) 49/292 0.3 64.8% intergenic (‑34/‑55) gfcA/insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
?CP009273 = 1973123 5 (0.080)intergenic (‑669/‑111) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 = 104600158 (0.950)50 (0.820) 43/292 0.5 62.5% intergenic (+15/+418) insB1/cspH IS1 transposase B/stress protein, member of the CspA‑family
?CP009273 1973115 = 2 (0.030)intergenic (‑661/‑119) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 = 362866358 (0.950)51 (0.830) 44/292 0.4 46.8% coding (463/1053 nt) yhiM acid resistance protein, inner membrane
?CP009273 3645396 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region