breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 260 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,184,6490AG57.2% 79.5 / inf 335noncoding (35/75 nt)ECDH1_RS15800tRNA‑TERM
*DH14,216,3820AG55.5% 225.7 / inf 586T234A (ACA→GCA) fdhFformate dehydrogenase subunit alpha
*DH14,194,5020AG54.5% 174.7 / inf 541Q195Q (CAA→CAGphnIalpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate synthase subunit PhnI
*DH14,547,8150TC54.5% 182.2 / inf 585Y80C (TAC→TGC) rhotranscription termination factor Rho
*DH13,882,7340AG54.2% 139.0 / inf 501Q76Q (CAA→CAGettAenergy‑dependent translational throttle protein EttA
*DH14,024,0300AG54.1% 135.5 / inf 487M97V (ATG→GTG) yjgRhypothetical protein
*DH1325,4730AG53.6% 125.9 / inf 628G8G (GGT→GGCrtcRFis family transcriptional regulator
*DH14,191,9760AG53.1% 111.9 / inf 581pseudogene (626/788 nt)phnDphosphonate ABC transporter permease
*DH14,328,6930AG52.8% 117.5 / inf 613F143S (TTC→TCC) rpoCDNA‑directed RNA polymerase subunit beta'
*DH12,476,5850TC52.8% 82.7 / inf 436pseudogene (1363/1446 nt)abgApeptidase M20
*DH14,213,5100AG52.7% 105.9 / inf 541T670A (ACA→GCA) mdtOmultidrug resistance protein MdtO
*DH1236,0860AG52.7% 82.4 / inf 553intergenic (+28/+26)ECDH1_RS01120/gorhypothetical protein/glutathione reductase
*DH12,571,2300CA52.6% 36.9 / inf 461R67S (CGC→AGC) clscardiolipin synthase A
*DH11,755,1480TC52.6% 72.1 / inf 467I24I (ATT→ATCcpsGphosphomannomutase
*DH12,580,9820TC52.5% 70.0 / inf 444V209A (GTT→GCT) adhEbifunctional acetaldehyde‑CoA/alcohol dehydrogenase
*DH11,012,6100CA52.3% 82.3 / inf 583T51N (ACC→AAC) truDtRNA pseudouridine(13) synthase TruD
*DH12,999,5880AT52.3% 66.5 / inf 390F269I (TTC→ATC) dacCD‑alanyl‑D‑alanine carboxypeptidase
*DH13,948,6440AG52.2% 74.9 / inf 540N102S (AAC→AGC) yjiLhypothetical protein
*DH13,983,0080AG52.2% 61.8 / inf 522Q285Q (CAA→CAGsgcXendoglucanase
*DH13,927,3560AG52.1% 56.5 / inf 499pseudogene (1089/3513 nt)mrrDEAD/DEAH box helicase

Marginal new junction evidence (lowest skew 10 of 57 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 2478888245 (0.480)201 (0.400) 144/290 3.0 46.5% noncoding (5/1204 nt) repeat_region repeat region
?DH1 2480088 = 225 (0.450)intergenic (‑73/‑63) insH1/fnr transposase/fumarate and nitrate reduction regulatory protein
* ? DH1 2991627 =259 (0.500)362 (0.700) 148/300 3.1 58.3% coding (328/1686 nt) ybjL transporter
?DH1 3575874 = NA (NA)noncoding (4/1201 nt) repeat_region repeat region
* ? DH1 = 2788747206 (0.400)213 (0.430) 142/292 3.2 50.3% noncoding (5/1206 nt) repeat_region repeat region
?DH1 2789947 = 221 (0.440)noncoding (1205/1206 nt) repeat_region repeat region
* ? DH1 299568 =NA (NA)221 (0.430) 143/300 3.4 41.3% noncoding (5/773 nt) repeat_region repeat region
?DH1 1463363 = 314 (0.610)coding (140/1203 nt) ackA acetate kinase
* ? DH1 = 300335NA (NA)194 (0.380) 131/300 4.4 37.7% noncoding (772/773 nt) repeat_region repeat region
?DH1 = 1463371 320 (0.620)coding (132/1203 nt) ackA acetate kinase
* ? DH1 2079930 =377 (0.730)174 (0.370) 119/276 4.5 31.4% coding (395/984 nt) pheS phenylalanine‑‑tRNA ligase subunit alpha
?DH1 = 2092203 413 (0.870)coding (838/2379 nt) ppsA phosphoenolpyruvate synthase
* ? DH1 1463363 =314 (0.610)213 (0.410) 128/300 4.6 40.4% coding (140/1203 nt) ackA acetate kinase
?DH1 2830574 = NA (NA)noncoding (5/773 nt) repeat_region repeat region
* ? DH1 = 1967355231 (0.450)165 (0.340) 121/286 4.7 42.8% noncoding (6/1206 nt) repeat_region repeat region
?DH1 1968555 = 221 (0.450)noncoding (1206/1206 nt) repeat_region repeat region
* ? DH1 = 1463371320 (0.620)195 (0.380) 124/300 5.0 37.9% coding (132/1203 nt) ackA acetate kinase
?DH1 3580753 = NA (NA)noncoding (3/976 nt) repeat_region repeat region
* ? DH1 1842888 =489 (0.950)135 (0.280)
+AAATGACTT
67/282 11.4 22.7% pseudogene (1117/1193 nt) ECDH1_RS08950 porin
?DH1 3303926 = NA (NA)pseudogene (76/183 nt) essD hypothetical protein