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breseq version 0.28.1 revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 260 shown) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | DH1 | 3,184,649 | 0 | A | G | 57.2% | 79.5 / inf | 335 | noncoding (35/75 nt) | ECDH1_RS15800 | tRNA‑TERM |
* | DH1 | 4,216,382 | 0 | A | G | 55.5% | 225.7 / inf | 586 | T234A (ACA→GCA) | fdhF | formate dehydrogenase subunit alpha |
* | DH1 | 4,194,502 | 0 | A | G | 54.5% | 174.7 / inf | 541 | Q195Q (CAA→CAG) | phnI | alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate synthase subunit PhnI |
* | DH1 | 4,547,815 | 0 | T | C | 54.5% | 182.2 / inf | 585 | Y80C (TAC→TGC) | rho | transcription termination factor Rho |
* | DH1 | 3,882,734 | 0 | A | G | 54.2% | 139.0 / inf | 501 | Q76Q (CAA→CAG) | ettA | energy‑dependent translational throttle protein EttA |
* | DH1 | 4,024,030 | 0 | A | G | 54.1% | 135.5 / inf | 487 | M97V (ATG→GTG) | yjgR | hypothetical protein |
* | DH1 | 325,473 | 0 | A | G | 53.6% | 125.9 / inf | 628 | G8G (GGT→GGC) | rtcR | Fis family transcriptional regulator |
* | DH1 | 4,191,976 | 0 | A | G | 53.1% | 111.9 / inf | 581 | pseudogene (626/788 nt) | phnD | phosphonate ABC transporter permease |
* | DH1 | 4,328,693 | 0 | A | G | 52.8% | 117.5 / inf | 613 | F143S (TTC→TCC) | rpoC | DNA‑directed RNA polymerase subunit beta' |
* | DH1 | 2,476,585 | 0 | T | C | 52.8% | 82.7 / inf | 436 | pseudogene (1363/1446 nt) | abgA | peptidase M20 |
* | DH1 | 4,213,510 | 0 | A | G | 52.7% | 105.9 / inf | 541 | T670A (ACA→GCA) | mdtO | multidrug resistance protein MdtO |
* | DH1 | 236,086 | 0 | A | G | 52.7% | 82.4 / inf | 553 | intergenic (+28/+26) | ECDH1_RS01120/gor | hypothetical protein/glutathione reductase |
* | DH1 | 2,571,230 | 0 | C | A | 52.6% | 36.9 / inf | 461 | R67S (CGC→AGC) | cls | cardiolipin synthase A |
* | DH1 | 1,755,148 | 0 | T | C | 52.6% | 72.1 / inf | 467 | I24I (ATT→ATC) | cpsG | phosphomannomutase |
* | DH1 | 2,580,982 | 0 | T | C | 52.5% | 70.0 / inf | 444 | V209A (GTT→GCT) | adhE | bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
* | DH1 | 1,012,610 | 0 | C | A | 52.3% | 82.3 / inf | 583 | T51N (ACC→AAC) | truD | tRNA pseudouridine(13) synthase TruD |
* | DH1 | 2,999,588 | 0 | A | T | 52.3% | 66.5 / inf | 390 | F269I (TTC→ATC) | dacC | D‑alanyl‑D‑alanine carboxypeptidase |
* | DH1 | 3,948,644 | 0 | A | G | 52.2% | 74.9 / inf | 540 | N102S (AAC→AGC) | yjiL | hypothetical protein |
* | DH1 | 3,983,008 | 0 | A | G | 52.2% | 61.8 / inf | 522 | Q285Q (CAA→CAG) | sgcX | endoglucanase |
* | DH1 | 3,927,356 | 0 | A | G | 52.1% | 56.5 / inf | 499 | pseudogene (1089/3513 nt) | mrr | DEAD/DEAH box helicase |
Marginal new junction evidence (lowest skew 10 of 57 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | DH1 | = 2478888 | 245 (0.480) | 201 (0.400) | 144/290 | 3.0 | 46.5% | noncoding (5/1204 nt) | repeat_region | repeat region |
? | DH1 | 2480088 = | 225 (0.450) | intergenic (‑73/‑63) | insH1/fnr | transposase/fumarate and nitrate reduction regulatory protein | |||||
* | ? | DH1 | 2991627 = | 259 (0.500) | 362 (0.700) | 148/300 | 3.1 | 58.3% | coding (328/1686 nt) | ybjL | transporter |
? | DH1 | 3575874 = | NA (NA) | noncoding (4/1201 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | = 2788747 | 206 (0.400) | 213 (0.430) | 142/292 | 3.2 | 50.3% | noncoding (5/1206 nt) | repeat_region | repeat region |
? | DH1 | 2789947 = | 221 (0.440) | noncoding (1205/1206 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | 299568 = | NA (NA) | 221 (0.430) | 143/300 | 3.4 | 41.3% | noncoding (5/773 nt) | repeat_region | repeat region |
? | DH1 | 1463363 = | 314 (0.610) | coding (140/1203 nt) | ackA | acetate kinase | |||||
* | ? | DH1 | = 300335 | NA (NA) | 194 (0.380) | 131/300 | 4.4 | 37.7% | noncoding (772/773 nt) | repeat_region | repeat region |
? | DH1 | = 1463371 | 320 (0.620) | coding (132/1203 nt) | ackA | acetate kinase | |||||
* | ? | DH1 | 2079930 = | 377 (0.730) | 174 (0.370) | 119/276 | 4.5 | 31.4% | coding (395/984 nt) | pheS | phenylalanine‑‑tRNA ligase subunit alpha |
? | DH1 | = 2092203 | 413 (0.870) | coding (838/2379 nt) | ppsA | phosphoenolpyruvate synthase | |||||
* | ? | DH1 | 1463363 = | 314 (0.610) | 213 (0.410) | 128/300 | 4.6 | 40.4% | coding (140/1203 nt) | ackA | acetate kinase |
? | DH1 | 2830574 = | NA (NA) | noncoding (5/773 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | = 1967355 | 231 (0.450) | 165 (0.340) | 121/286 | 4.7 | 42.8% | noncoding (6/1206 nt) | repeat_region | repeat region |
? | DH1 | 1968555 = | 221 (0.450) | noncoding (1206/1206 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | = 1463371 | 320 (0.620) | 195 (0.380) | 124/300 | 5.0 | 37.9% | coding (132/1203 nt) | ackA | acetate kinase |
? | DH1 | 3580753 = | NA (NA) | noncoding (3/976 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | 1842888 = | 489 (0.950) | 135 (0.280) +AAATGACTT |
67/282 | 11.4 | 22.7% | pseudogene (1117/1193 nt) | ECDH1_RS08950 | porin |
? | DH1 | 3303926 = | NA (NA) | pseudogene (76/183 nt) | essD | hypothetical protein |