breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 38 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,1970GT36.1% 91.8 / 15.0 80intergenic (‑32/+3)leuV/leuPtRNA‑Leu/tRNA‑Leu
*NC_0009132,947,4670AC35.0% 189.8 / 151.3 117intergenic (+4/‑30)metZ/metWtRNA‑Met/tRNA‑Met
*NC_0009131,708,9010AC34.0% 139.4 / 64.7 205R661R (CGA→CGCrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009131,552,1910CA31.3% 680.9 / 115.4 383intergenic (+200/+207)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
*NC_0009134,606,1920GC31.1% 127.4 / 23.7 95intergenic (‑27/+8)leuV/leuPtRNA‑Leu/tRNA‑Leu
*NC_000913422,4290TG29.8% 523.8 / 92.8 336intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_0009132,947,5910A.28.7% 198.1 / 82.9 80intergenic (+18/‑16)metW/metVtRNA‑Met/tRNA‑Met
*NC_0009131,708,9300CA28.3% 343.7 / 63.4 215P671Q (CCG→CAG) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009132,947,4811.A27.8% 173.4 / 102.7 72intergenic (+18/‑16)metZ/metWtRNA‑Met/tRNA‑Met
*NC_0009132,560,9960GT27.2% 584.0 / 68.4 332intergenic (+98/‑372)yffL/yffMCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
*NC_0009132,947,4650T.26.9% 381.2 / 154.0 157intergenic (+2/‑32)metZ/metWtRNA‑Met/tRNA‑Met
*NC_0009131,708,8050TA26.7% 96.0 / 23.9 125R629R (CGT→CGArsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009134,296,0600CT26.1% 706.8 / inf 542intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_000913422,4320TG25.8% 643.5 / 67.4 336intergenic (+73/‑86)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_0009131,708,8800CA25.1% 251.4 / 22.6 178A654A (GCC→GCArsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009133,269,9940GT25.0% 225.5 / 18.1 164intergenic (+392/+631)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_0009133,270,1670CG25.0% 311.9 / 41.9 213intergenic (+565/+458)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_000913422,4350TG24.8% 668.2 / 57.1 335intergenic (+76/‑83)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_0009131,708,9100GC24.3% 334.6 / 34.6 207A664A (GCG→GCCrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_000913696,5340GT23.5% 248.1 / 29.0 119intergenic (‑30/+8)glnX/glnVtRNA‑Gln/tRNA‑Gln

Marginal new junction evidence (lowest skew 10 of 38 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1207805241 (0.600)176 (0.490) 127/268 3.7 45.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 208 (0.580)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1207790 =237 (0.590)149 (0.410) 113/268 5.7 40.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 229 (0.630)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 696863 =151 (0.370)89 (0.240)
+GAAGAAACAATC
43/276 25.3 25.0% intergenic (‑33/+2) glnW/glnU tRNA‑Gln/tRNA‑Gln
?NC_000913 = 697134 429 (1.060)intergenic (‑1/+379) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 1287084143 (0.350)65 (0.190) 37/260 26.3 24.3% intergenic (+558/+3) narI/tpr nitrate reductase 1, gamma (cytochrome b(NR)) subunit/protamine‑like protein
?NC_000913 1287557 = 281 (0.800)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 781369 =242 (0.600)124 (0.320)
+GAAG
44/292 26.4 38.0% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 174 (0.430)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 781577 =268 (0.660)203 (0.520)
+GAAG
43/292 26.8 48.6% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 174 (0.430)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 780554 =477 (1.180)95 (0.240)
+GAAG
43/292 26.8 23.1% noncoding (1/76 nt) lysT tRNA‑Lys
?NC_000913 = 2521006 174 (0.430)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 781369 =242 (0.600)104 (0.260)
+G
40/298 28.7 36.9% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521128 116 (0.290)intergenic (+2/‑45) valX/valY tRNA‑Val/tRNA‑Val
* ? NC_000913 781369 =242 (0.600)127 (0.320)
+GAG
39/294 28.7 38.4% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 174 (0.430)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 781446227 (0.560)46 (0.130) 29/260 30.2 13.8% intergenic (+2/‑131) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 2521487 = 376 (1.070)intergenic (+159/+106) lysV/xapR tRNA‑Lys/transcriptional activator of xapAB