breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 44 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913377,4311.A79.1% 91.3 / 46.7 67intergenic (+120/+104)yaiL/frmBDUF2058 family protein/S‑formylglutathione hydrolase
*NC_000913377,4320CG79.1% 32.4 / 39.5 67intergenic (+121/+103)yaiL/frmBDUF2058 family protein/S‑formylglutathione hydrolase
*NC_000913696,4700CT78.6% 571.2 / 126.4 292noncoding (35/75 nt)glnXtRNA‑Gln
*NC_0009134,040,6230GA52.6% 298.6 / inf 880noncoding (107/120 nt)rrfA5S ribosomal RNA of rrnA operon
*NC_0009134,171,7530GA45.8% 17.9 / 23.9 27noncoding (117/120 nt)rrfB5S ribosomal RNA of rrnB operon
*NC_0009132,947,5910A.38.6% 175.1 / 122.7 83intergenic (+18/‑16)metW/metVtRNA‑Met/tRNA‑Met
*NC_0009132,947,4811.A37.3% 113.1 / 135.4 67intergenic (+18/‑16)metZ/metWtRNA‑Met/tRNA‑Met
*NC_0009134,295,9610CT35.3% 386.8 / inf 428intergenic (+167/+475)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009134,171,7670GA35.0% 26.8 / 14.8 21intergenic (+11/‑290)rrfB/murB5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
*NC_0009132,947,4670AC34.2% 188.9 / 150.8 120intergenic (+4/‑30)metZ/metWtRNA‑Met/tRNA‑Met
*NC_0009133,269,9940GT31.9% 188.4 / 25.4 161intergenic (+392/+631)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_0009134,296,0600CT29.6% 541.2 / inf 539intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009131,552,1910CA29.2% 739.3 / 113.0 431intergenic (+200/+207)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
*NC_0009132,818,1580TA29.0% 274.5 / 116.9 187intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg
*NC_0009132,304,5220GT28.9% 126.7 / 76.3 115intergenic (+129/+586)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
*NC_0009131,708,9010AC28.6% 221.4 / 56.0 219R661R (CGA→CGCrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009132,304,5230GC28.3% 133.9 / 79.8 115intergenic (+130/+585)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
*NC_0009131,708,9300CA28.1% 378.2 / 55.9 230P671Q (CCG→CAG) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_000913422,4290TG27.7% 628.6 / 90.9 356intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_0009133,270,1670CG27.2% 327.5 / 53.4 243intergenic (+565/+458)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I

Marginal new junction evidence (lowest skew 10 of 34 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 781446242 (0.570)56 (0.150) 40/260 28.3 17.4% intergenic (+2/‑131) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 2521487 = 323 (0.880)intergenic (+159/+106) lysV/xapR tRNA‑Lys/transcriptional activator of xapAB
* ? NC_000913 = 2357690308 (0.730)64 (0.160)
+AATTAGAAGGTT
39/276 30.6 18.4% coding (787/900 nt) yfaD transposase_31 family protein
?NC_000913 2357691 = 308 (0.730)coding (788/900 nt) yfaD transposase_31 family protein
* ? NC_000913 = 781446242 (0.570)49 (0.130) 33/260 31.8 15.5% intergenic (+2/‑131) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 781811 = 326 (0.890)intergenic (+159/‑274) lysQ/nadA tRNA‑Lys/quinolinate synthase, subunit A
* ? NC_000913 780554 =437 (1.040)116 (0.280)
+G
41/298 32.1 30.8% noncoding (1/76 nt) lysT tRNA‑Lys
?NC_000913 = 2521129 87 (0.210)intergenic (+3/‑44) valX/valY tRNA‑Val/tRNA‑Val
* ? NC_000913 780554 =437 (1.040)91 (0.220)
+GAAG
39/292 32.4 24.0% noncoding (1/76 nt) lysT tRNA‑Lys
?NC_000913 = 2521006 156 (0.370)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 781577 =286 (0.680)228 (0.540)
+G
40/298 32.6 55.2% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521129 87 (0.210)intergenic (+3/‑44) valX/valY tRNA‑Val/tRNA‑Val
* ? NC_000913 781369 =266 (0.630)159 (0.380)
+G
40/298 32.6 47.6% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521129 87 (0.210)intergenic (+3/‑44) valX/valY tRNA‑Val/tRNA‑Val
* ? NC_000913 781577 =286 (0.680)203 (0.490)
+GAAG
38/292 32.9 48.6% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 156 (0.370)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 781369 =266 (0.630)131 (0.320)
+GAAG
38/292 32.9 39.0% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 156 (0.370)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 1287084170 (0.400)56 (0.150) 30/260 33.5 19.4% intergenic (+558/+3) narI/tpr nitrate reductase 1, gamma (cytochrome b(NR)) subunit/protamine‑like protein
?NC_000913 1287557 = 317 (0.870)noncoding (66/85 nt) tyrT tRNA‑Tyr