breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 55,007 C→A G701G (GGG→GGT lptD ← lipopolysaccharide assembly protein LptD
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 720,492 T→C intergenic (‑32/‑91) speF ← / → ybfK ornithine decarboxylase, degradative/uncharacterized protein YbfK
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,086,840 C→A C336F (TGC→TTC)  pgaC ← poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaC
RA 1,220,454 C→A pseudogene (854/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,780,197 C→A A194E (GCG→GAG)  ydiQ → putative electron transfer flavoprotein subunit YdiQ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,166,562 G→T A299S (GCA→TCA)  yegQ → putative peptidase YegQ
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,068,851 G→T intergenic (‑43/+96) argO ← / ← mscS L‑arginine exporter/small conductance mechanosensitive channel MscS
RA 3,118,565 G→T A183E (GCG→GAG)  yghJ ← putative lipoprotein YghJ
RA 3,269,368 C→A T318T (ACC→ACA yhaC → uncharacterized protein YhaC
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,462,869 G→T E176* (GAG→TAG)  gspJ → Type II secretion system protein GspJ
RA 3,550,043 C→A A10S (GCG→TCG)  malQ ← 4‑alpha‑glucanotransferase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,911,319 C→A Q69H (CAG→CAT pstS ← phosphate ABC transporter periplasmic binding protein
RA 4,160,832 G→T intergenic (‑42/‑235) sthA ← / → fabR soluble pyridine nucleotide transhydrogenase/DNA‑binding transcriptional repressor FabR
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
JC JC 4,640,748 IS5 (+) +4 bp intergenic (+206/‑191) yjjY → / → yjtD protein YjjY/putative rRNA methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102945 3104055 1111 10 [9] [9] 11 [mutY] [mutY]
* * ÷ NC_000913 3423755–3424528 3424528 1–774 10 [9] [9] 11 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =18 (0.420)53 (1.390) 38/246 0.1 83.5% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 5 (0.130)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780518 (0.420)26 (0.680) 24/246 0.5 71.3% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 5 (0.130)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)42 (1.000) 32/270 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =77 (1.780)73 (1.700) 47/276 0.0 64.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.070)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)54 (1.250) 38/278 0.1 94.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA