breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 7,541 C→A R140L (CGC→CTC)  yaaJ ← putative transporter YaaJ
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 259,264 A→G G279G (GGT→GGC phoE ← outer membrane porin PhoE
RA 275,977 C→A S218* (TCG→TAG)  mmuP → S‑methyl‑L‑methionine transporter
RA 293,318 G→T L201I (CTT→ATT)  yagL ← CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL
RA 380,385 +GA coding (458/774 nt) yaiO ← outer membrane protein YaiO
RA 399,371 G→T intergenic (‑38/‑222) yaiY ← / → yaiZ inner membrane protein/DUF2754 domain‑containing protein YaiZ
RA 476,195 C→A A59A (GCG→GCT atl ← DNA base‑flipping protein
RA 507,468 G→C P205A (CCG→GCG)  ybaP ← TraB family protein YbaP
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,910,862 C→A S196Y (TCT→TAT)  yebQ → putative transporter YebQ
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,028,016 A→C M1G (GTG→GGG) † dgcQ ← putative diguanylate cyclase DgcQ
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,792,806 G→A P24P (CCG→CCA gabT → 4‑aminobutyrate aminotransferase GabT
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,393,854 A→G L802S (TTA→TCA)  yhdP ← AsmA2 domain‑containing protein
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,844,007 C→A A15D (GCT→GAT)  adeD → adenine deaminase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,566,757 C→A D164Y (GAT→TAT)  yjiN ← DUF445 domain‑containing protein YjiN

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196259 1211433 15175 31 [29] [27] 35 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102960 3104069 1110 31 [29] [29] 31 mutY mutY
* * ÷ NC_000913 3423770–3424232 3424539–3424239 8–770 32 [27] [30] 31 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)78 (0.770) 52/268 0.6 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =139 (1.330)156 (1.500) 71/274 0.1 68.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)119 (1.140) 63/276 0.3 98.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA