breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,179,596 G→T R26L (CGT→CTT)  cobB → protein‑lysine deacetylase/desuccinylase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,462,256 C→A F44L (TTC→TTA paaK → phenylacetate‑CoA ligase
RA 1,536,278 G→T L114M (CTG→ATG)  narV ← nitrate reductase Z subunit gamma
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,698,692 G→T R163R (CGC→CGA malI ← DNA‑binding transcriptional repressor MalI
RA 1,747,774 G→T G215V (GGT→GTT)  ydhS → FAD/NAD(P) binding domain‑containing protein YdhS
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,319,044 C→A G278V (GGC→GTC)  rcsC ← sensory histidine kinase RcsC
RA 2,831,760 G→T T177K (ACG→AAG)  norR ← DNA‑binding transcriptional dual regulator NorR
RA 3,114,910 G→T S1401R (AGC→AGA yghJ ← putative lipoprotein YghJ
RA 3,155,165 G→T R18R (CGC→CGA yqhC ← DNA‑binding transcriptional activator YqhC
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,659,977 G→T R249L (CGC→CTC)  mdtE → multidrug efflux pump membrane fusion protein MdtE
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,994,150 G→T D101Y (GAT→TAT)  yzcX → protein YzcX
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,392,323 G→T intergenic (+174/‑37) orn → / → glyV oligoribonuclease/tRNA‑Gly

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196275 1211428 15154 10 [9] [8] 12 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102949 3104069 1121 10 [9] [9] 11 mutY mutY
* * ÷ NC_000913 3423770–3424233 3424525–3424238 6–756 12 [9] [9] 10 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)55 (1.240) 45/270 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =86 (1.880)77 (1.690) 46/276 0.0 63.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.090)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)63 (1.380) 43/278 0.1 92.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.110)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =32 (0.700)13 (0.300) 12/260 1.9 27.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 40 (0.930)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269032 (0.700)11 (0.260) 11/260 2.1 23.9% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 40 (0.930)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit