breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC40.0% 9.4 / 13.1 15intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009134,604,3440CT37.4% 219.5 / 102.7 166S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009132,001,9180AC35.0% 172.1 / 44.0 97intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_0009133,206,4350CT35.0% 121.2 / 73.7 100intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009134,111,7470GT30.3% 231.0 / 60.2 99N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ
*NC_0009133,206,4440TA27.3% 176.7 / 40.9 99intergenic (‑188/‑19)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009134,568,5040TG25.9% 388.6 / 24.6 176T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_0009134,111,7520TC25.6% 238.2 / 28.4 121K155E (AAA→GAA) yiiQDUF1454 domain‑containing protein YiiQ
*NC_0009134,287,7190TA25.4% 285.4 / 69.0 142intergenic (‑348/‑45)acs/nrfAacetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase
*NC_000913372,6400TG24.2% 226.2 / 26.4 99L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
*NC_0009131,825,0610GA24.1% 176.8 / 28.2 83intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009131,911,7540TG23.9% 157.7 / 13.1 67T275P (ACC→CCC) htpXzinc dependent endoprotease
*NC_000913650,8090TG23.8% 335.9 / 44.6 143T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_0009133,556,2070TC23.5% 181.1 / 23.9 85S215G (AGT→GGT) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_00091329,4960TA23.4% 261.5 / 42.3 111intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_000913646,7470TG23.4% 259.5 / 51.1 112T255P (ACC→CCC) citGtriphosphoribosyl‑dephospho‑CoA synthase
*NC_0009134,429,9840TG22.8% 371.8 / 50.2 150L41V (TTG→GTG) cycAserine/alanine/glycine/cycloserine:H(+)symporter
*NC_0009132,628,6250AC22.4% 142.2 / 17.1 58V105G (GTC→GGC) pdeFcyclic di‑GMP phosphodiesterase PdeF
*NC_0009133,404,2150TG22.1% 455.2 / 54.6 181G244G (GGT→GGGyhdHacrylyl‑CoA reductase
*NC_0009133,556,2050AC21.9% 214.7 / 13.6 82S215R (AGT→AGG) ‡rtcARNA 3'‑terminal phosphate cyclase

Marginal new junction evidence (lowest skew 10 of 36 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 454269094 (0.500)31 (0.180) 27/256 3.9 18.1% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 192 (1.100)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 4542682 =115 (0.610)27 (0.150) 21/256 4.9 15.3% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 192 (1.100)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 375102 =NA (NA)12 (0.080) 10/214 6.8 NA noncoding (17/36 nt) other REP31d
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 375203 =NA (NA)9 (0.060) 9/214 7.1 NA noncoding (17/36 nt) other REP31f
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 2765420 =249 (1.330)6 (0.050) 6/186 7.6 3.3% intergenic (‑267/+93) abpA/yfjM CP4‑57 prophage; anti‑bacteriophage protein/CP4‑57 prophage; protein YfjM
?NC_000913 = 2765411 182 (1.430)intergenic (‑258/+102) abpA/yfjM CP4‑57 prophage; anti‑bacteriophage protein/CP4‑57 prophage; protein YfjM
* ? NC_000913 = 374963NA (NA)8 (0.060) 6/188 7.7 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375001 =NA (NA)4 (0.030) 4/214 9.4 NA noncoding (17/36 nt) other REP31b
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 374922 =NA (NA)3 (0.020) 3/200 9.6 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377430 = NA (NA)intergenic (+119/+105) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 16731NA (NA)4 (0.030) 4/222 9.6 3.8% noncoding (1345/1345 nt) IS186 repeat region
?NC_000913 = 2514272 100 (0.660)intergenic (+28/‑59) nupC/insL‑3 nucleoside:H(+) symporter NupC/putative IS186/IS421 transposase
* ? NC_000913 545672 =206 (1.100)5 (0.030) 5/254 9.7 2.5% coding (692/1050 nt) allD ureidoglycolate dehydrogenase
?NC_000913 = 545668 197 (1.130)coding (696/1050 nt) allD ureidoglycolate dehydrogenase