breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,052,503 G→T S287* (TCA→TAA)  yccM ← putative 4Fe‑4S membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,508,798 G→T intergenic (+56/+36) rlhA → / ← yncJ 23S rRNA 5‑hydroxycytidine C2501 synthase/protein YncJ
JC 1,584,358 Δ17 bp coding (2113‑2129/2280 nt) ydeP ← putative oxidoreductase YdeP
RA 1,619,094 A→G intergenic (‑186/‑26) marC ← / → marR inner membrane protein MarC/DNA‑binding transcriptional repressor MarR
RA 1,658,376 G→T R103L (CGC→CTC)  ynfE → putative selenate reductase YnfE
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,755,649 G→T noncoding (57/363 nt) ssrA → tmRNA
RA 3,011,426 A→C intergenic (+13/‑35) hyuA → / → yqeA phenylhydantoinase/putative amino acid kinase YqeA
RA 3,213,155 G→T D37Y (GAT→TAT)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,597,702 T→C intergenic (‑142/‑282) livK ← / → panZ L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein/PanD maturation factor
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,281,333 A→C K452Q (AAA→CAA)  yjcE → putative transporter YjcE
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,539,822 C→A intergenic (‑321/‑1135) nanC ← / → fimB N‑acetylneuraminic acid outer membrane channel/regulator for fimA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196292 1211431 15140 18 [15] [17] 24 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102973 3104055 1083 19 [17] [16] 18 [mutY] [mutY]
* * ÷ NC_000913 3423755–3424231 3424530–3424238 8–776 19 [16] [17] 19 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)130 (1.720) 68/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =135 (1.740)129 (1.670) 68/274 0.0 65.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.060)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)93 (1.200) 45/276 0.4 95.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA