breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
RA | 364,378 | T→C | Y643C (TAC→TGC) | lacZ ← | beta‑galactosidase |
RA | 889,923 | A→G | intergenic (‑104/‑166) | ybjL ← / → ybjM | putative transport protein YbjL/putative inner membrane protein |
RA | 927,648 | C→A | L516F (TTG→TTT) | cydC ← | glutathione/L‑cysteine ABC exporter subunit CydC |
RA | 1,169,682 | C→T | Q231Q (CAG→CAA) | ldtC ← | L,D‑transpeptidase YcfS |
RA | 1,196,220 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase |
RA | 1,196,232 | C→T | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase |
RA | 1,196,245 | T→C | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
RA | 1,196,247 | A→G | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
RA | 1,606,295 | A→G | T66A (ACC→GCC) | lsrF → | 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase |
RA | 1,678,604 | G→T | A60S (GCA→TCA) | ydgH → | DUF1471 domain‑containing protein YdgH |
RA | 1,688,480 | T→A | intergenic (‑103/‑96) | fumA ← / → manA | fumarase A/mannose‑6‑phosphate isomerase |
RA | 1,720,181 | A→C | V102V (GTA→GTC) | slyB → | outer membrane lipoprotein SlyB |
JC JC | 1,879,829 | Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp | coding (115‑120/360 nt) | yeaR ← | DUF1971 domain‑containing protein YeaR |
MC JC | 1,978,503 | Δ776 bp | insB‑5–insA‑5 | insB‑5, insA‑5 | |
RA | 2,173,363 | Δ2 bp | pseudogene (915‑916/1358 nt) | gatC ← | galactitol‑specific PTS enzyme IIC component |
RA | 2,519,252 | G→A | intergenic (+47/+5) | yfeD → / ← gltX | putative DNA‑binding transcriptional regulator YfeD/glutamate‑‑tRNA ligase |
RA | 2,769,719 | G→T | R6L (CGC→CTC) | yfjR → | CP4‑57 prophage; putative DNA‑binding transcriptional regulator |
RA | 2,835,689 | C→A | A579A (GCG→GCT) | hypF ← | carbamoyltransferase HypF |
RA | 3,354,487 | C→T | intergenic (‑437/‑238) | yhcC ← / → gltB | radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB |
RA | 3,546,932 | T→A | V125E (GTG→GAG) | gntT → | high‑affinity gluconate transporter |
RA | 3,560,455 | +G | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
RA | 3,815,883 | +C | coding (667/687 nt) | rph ← | truncated RNase PH |
RA | 4,261,710 | C→A | S14R (AGC→AGA) | dusA → | tRNA‑dihydrouridine synthase A |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
RA | 4,319,016 | G→T | D197E (GAC→GAA) | phnJ ← | carbon‑phosphorus lyase core complex subunit PhnJ |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1196258 | 1211433 | 15176 | 18 [16] | [14] 19 | [icd]–[icdC] | [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC] |
* | * | ÷ | NC_000913 | 3102967 | 3104051 | 1085 | 18 [17] | [17] 19 | [mutY] | [mutY] |
* | * | ÷ | NC_000913 | 3423770–3424234 | 3424538–3424242 | 9–769 | 23 [17] | [17] 18 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 65 (1.090) | 51/270 | 0.1 | 100% | intergenic (+253/‑33) | ychE/insH21 | putative inner membrane protein/IS5 transposase and trans‑activator |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+151/‑484) | insH21/oppA | IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 3363001 = | 106 (1.730) | 87 (1.430) | 60/276 | 0.0 | 61.9% | coding (195/2382 nt) | yhcD | putative fimbrial usher protein YhcD |
? | NC_000913 | 3766087 = | 2 (0.030) | coding (3905/4134 nt) | rhsA | rhs element protein RhsA | |||||
* | ? | NC_000913 | = 3653230 | NA (NA) | 65 (1.060) | 43/278 | 0.3 | 98.5% | noncoding (1/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 3766089 | 1 (0.020) | coding (3907/4134 nt) | rhsA | rhs element protein RhsA | |||||
* | ? | NC_000913 | 4542682 = | 47 (0.770) | 16 (0.280) | 14/260 | 2.7 | 25.0% | intergenic (+49/‑433) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
? | NC_000913 | 4542996 = | 52 (0.910) | intergenic (+363/‑119) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |