breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 364,378 T→C Y643C (TAC→TGC)  lacZ ← beta‑galactosidase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 927,648 C→A L516F (TTG→TTT cydC ← glutathione/L‑cysteine ABC exporter subunit CydC
RA 1,169,682 C→T Q231Q (CAG→CAA ldtC ← L,D‑transpeptidase YcfS
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,606,295 A→G T66A (ACC→GCC)  lsrF → 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,688,480 T→A intergenic (‑103/‑96) fumA ← / → manA fumarase A/mannose‑6‑phosphate isomerase
RA 1,720,181 A→C V102V (GTA→GTC slyB → outer membrane lipoprotein SlyB
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,519,252 G→A intergenic (+47/+5) yfeD → / ← gltX putative DNA‑binding transcriptional regulator YfeD/glutamate‑‑tRNA ligase
RA 2,769,719 G→T R6L (CGC→CTC)  yfjR → CP4‑57 prophage; putative DNA‑binding transcriptional regulator
RA 2,835,689 C→A A579A (GCG→GCT hypF ← carbamoyltransferase HypF
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,546,932 T→A V125E (GTG→GAG)  gntT → high‑affinity gluconate transporter
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,319,016 G→T D197E (GAC→GAA phnJ ← carbon‑phosphorus lyase core complex subunit PhnJ

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196258 1211433 15176 18 [16] [14] 19 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102967 3104051 1085 18 [17] [17] 19 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424234 3424538–3424242 9–769 23 [17] [17] 18 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)65 (1.090) 51/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =106 (1.730)87 (1.430) 60/276 0.0 61.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)65 (1.060) 43/278 0.3 98.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =47 (0.770)16 (0.280) 14/260 2.7 25.0% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 52 (0.910)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit