breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,023,757 G→T P43T (CCG→ACG)  yccS ← putative transporter YccS
RA 1,251,180 C→A Q39K (CAG→AAG)  dhaR → DNA‑binding transcriptional dual regulator DhaR
RA 1,397,145 C→A intergenic (+53/‑220) insH‑4 → / → ynaJ IS5 transposase and trans‑activator/DUF2534 domain‑containing protein YnaJ
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,165,614 C→A intergenic (+93/‑54) yegP → / → yegQ DUF1508 domain‑containing protein YegP/putative peptidase YegQ
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,984,362 C→A intergenic (‑238/+49) kduI ← / ← yqeF 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase/putative acyltransferase
RA 3,028,082 C→A A321E (GCG→GAG)  ghxQ → guanine/hypoxanthine transporter GhxQ
RA 3,116,464 +G coding (2649/4563 nt) yghJ ← putative lipoprotein YghJ
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,622,646 G→T G1152V (GGC→GTC)  rhsB → rhs element protein RhsB
RA 3,802,739 G→T T107N (ACT→AAT)  waaO ← UDP‑D‑glucose:(glucosyl)LPS alpha‑1,3‑glucosyltransferase
JC JC 3,806,379 IS1 (–) +9 bp coding (681‑689/1125 nt) waaG ← lipopolysaccharide glucosyltransferase I
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,878,666 G→T P485Q (CCG→CAG)  gyrB ← DNA gyrase subunit B
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,277,632 G→T R55L (CGA→CTA)  soxR → DNA‑binding transcriptional dual regulator SoxR
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
MC JC 4,503,072 Δ14 bp coding (347‑360/1329 nt) insG ← KpLE2 phage‑like element; IS4 putative transposase
RA 4,576,361 C→A pseudogene (2227/2721 nt) yjiV → putative uncharacterized protein YjiV

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102942 3104068 1127 29 [28] [28] 29 mutY mutY
* * ÷ NC_000913 3423748–3424234 3424550–3424238 5–803 29 [26] [28] 30 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =18 (0.200)131 (1.640) 70/244 0.0 90.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 12 (0.150)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780518 (0.200)68 (0.850) 43/244 0.5 83.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 12 (0.150)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)118 (1.350) 67/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =149 (1.650)128 (1.430) 64/274 0.1 62.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)104 (1.150) 58/276 0.2 94.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 6 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA