breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 343,261 G→T L126L (CTG→CTT yahK → aldehyde reductase, NADPH‑dependent
RA 724,908 C→T L24L (CTG→CTA kdpC ← K(+) transporting P‑type ATPase subunit KdpC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,267,926 A→T T203S (ACC→TCC)  ychA → transglutaminase‑like/TPR repeat‑containing protein
RA 1,451,355 A→T intergenic (‑6/+242) tynA ← / ← paaZ copper‑containing amine oxidase/oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,473,160 Δ1 bp pseudogene (2642/3495 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,721,915 Δ1 bp coding (652/858 nt) ydhJ → putative membrane fusion protein YdhJ
RA 1,802,435 C→A D46Y (GAT→TAT)  thrS ← threonine‑‑tRNA ligase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,888,053 C→A intergenic (‑62/+8) rnd ← / ← fadD RNase D/fatty acyl‑CoA synthetase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,227,228 C→A G15C (GGC→TGC)  yohF ← putative oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
RA 2,564,480 T→C intergenic (+108/‑43) yffR → / → yffS CPZ‑55 prophage; uncharacterized protein YffR/CPZ‑55 prophage; uncharacterized protein YffS
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,958,515 T→A D182E (GAT→GAA ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 4,011,002 C→T intergenic (+2/‑74) yigL → / → bioP phosphosugar phosphatase YigL/biotin transporter
JC JC 4,087,498 IS5 (+) +4 bp coding (497‑500/1056 nt) yiiG → DUF3829 domain‑containing lipoprotein YiiG
RA 4,233,447 T→G intergenic (‑214/‑311) lysC ← / → pgi aspartate kinase III/glucose‑6‑phosphate isomerase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196270 1211431 15162 22 [20] [20] 21 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423770–3424234 3424525–3424238 5–756 21 [17] [13] 28 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)84 (1.140) 55/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =100 (1.310)114 (1.510) 65/274 0.1 69.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)83 (1.090) 54/276 0.2 98.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.010)coding (3907/4134 nt) rhsA rhs element protein RhsA