breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,020,961 A→G intergenic (‑42/‑177) sulA ← / → sxy cell division inhibitor SulA/transcriptional coactivator for CRP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,600,426 C→T A272A (GCG→GCA lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,647,030 C→T intergenic (+293/+144) flxA → / ← ydfW Qin prophage; protein FlxA/Qin prophage; uncharacterized protein YdfW
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,953,436 Δ1 bp coding (735/744 nt) cmoA → carboxy‑S‑adenosyl‑L‑methionine synthase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,618,360 T→C P139P (CCA→CCG hda ← inibitor of reinitiation of DNA replication
RA 2,798,780 A→G intergenic (‑285/‑384) stpA ← / → alaE DNA‑binding transcriptional repressor StpA with RNA chaperone activity/L‑alanine exporter
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
MC JC 4,142,990 Δ235 bp coding (461‑695/1080 nt) frwC → putative PTS enzyme IIC component FrwC
RA 4,267,869 C→T Y252Y (TAC→TAT tyrB → tyrosine aminotransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,462,837 T→C intergenic (‑177/+217) nrdD ← / ← treC anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196304 1211419 15116 4 [2] [3] 4 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1437231 1437359 129 4 [3] [3] 4 [ydbK] [ydbK]
* * ÷ NC_000913 1438142 1438429 288 4 [3] [2] 4 ydbK putative pyruvate‑flavodoxin oxidoreductase
* * ÷ NC_000913 1440210 1440395 186 4 [3] [3] 4 ydbK putative pyruvate‑flavodoxin oxidoreductase
* * ÷ NC_000913 1440515 1440713 199 4 [3] [2] 4 ydbK putative pyruvate‑flavodoxin oxidoreductase
* * ÷ NC_000913 1441040 1441328 289 4 [3] [3] 4 ydbJ–[hslJ] ydbJ,[hslJ]
* * ÷ NC_000913 1441600 1441778 179 4 [3] [2] 4 [hslJ] [hslJ]
* * ÷ NC_000913 1442562 1442816 255 4 [3] [3] 4 ldhA D‑lactate dehydrogenase
* * ÷ NC_000913 1444109 1444328 220 4 [3] [3] 4 ydbH PF11739 family protein YdbH
* * ÷ NC_000913 1447963 1448270 308 4 [1] [3] 4 feaB phenylacetaldehyde dehydrogenase
* * ÷ NC_000913 1454172 1454547 376 5 [2] [3] 4 paaA phenylacetyl‑CoA 1,2‑epoxidase, monooxygenase subunit
* * ÷ NC_000913 1455845 1456040 196 4 [3] [3] 5 [paaC]–[paaD] [paaC],[paaD]
* * ÷ NC_000913 1458328 1458620 293 4 [2] [3] 4 paaG putative ring 1,2‑epoxyphenylacetyl‑CoA isomerase (oxepin‑CoA forming)
* * ÷ NC_000913 1458806 1459654 849 4 [3] [3] 4 [paaG]–[paaH] [paaG],[paaH]
* * ÷ NC_000913 1461489 1461573 85 4 [3] [3] 4 paaJ beta‑ketoadipyl‑CoA thiolase
* * ÷ NC_000913 1467971–1469238 1590764 121527–122794 5 [3] [0] 1280 [insD‑2]–yneL [insD‑2],insC‑2,ydbA,insI‑2,ydbA,pdxI,ydbD,ynbA,ynbB,ynbC,ynbD,azoR,hrpA,ydcF,aldA,gapC,cybB,rydC,ydcA,hokB,mokB,sokB,trg,ydcI,ydcJ,opgD,ydcH,rimL,ydcK,tehA,tehB,ydcL,insQ,ydcO,sutR,rlhA,yncJ,hicA,hicB,ydcR,ydcS,ydcT,ydcU,ydcV,patD,yncL,ortT,ydcY,ydcZ,mnaT,curA,mcbR,yncD,yncE,ansP,yncG,yncH,rhsE,ydcD,yncO,yncI,ydcC,pptA,yddH,nhoA,yddE,narV,narW,narY,narZ,narU,yddJ,yddK,yddL,yddG,fdnG,fdnH,fdnI,yddM,adhP,maeA,sra,bdm,osmC,ddpF,ddpD,ddpC,ddpB,ddpA,ddpX,dosP,dosC,yddY,yddW,gadC,gadB,pqqL,yddB,yddA,yneP,ydeM,ydeN,ydeO,safA,ydeP,ydeQ,ydeR,ydeS,ydeT,yneL
* * ÷ NC_000913 3423795–3424230 3424523–3424238 9–729 4 [3] [3] 4 [rrlD] [rrlD]
* * ÷ NC_000913 3751949 3751971 23 4 [3] [2] 4 yiaT/yiaU outer membrane protein YiaT/putative LysR‑type DNA‑binding transcriptional regulator YiaU

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1432951NA (NA)7 (0.170) 7/276 1.8 NA coding (541/576 nt) tfaR Rac prophage; putative tail fiber assembly protein TfaR
?NC_000913 = 1433168 NA (NA)coding (507/591 nt) pinR Rac prophage; putative site‑specific recombinase
* ? NC_000913 1590765 =0 (0.000)1257 (32.560) 178/266 0.0 99.4% intergenic (‑229/+89) yneL/hipA putative HTH‑type transcriptional regulator YneL/serine/threonine kinase HipA
?NC_000913 = 1647024 15 (0.390)intergenic (+287/+150) flxA/ydfW Qin prophage; protein FlxA/Qin prophage; uncharacterized protein YdfW
* ? NC_000913 3363001 =40 (1.000)34 (0.850) 25/274 0.2 62.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)34 (0.850) 27/276 0.1 87.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.120)coding (3907/4134 nt) rhsA rhs element protein RhsA