breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA CP000731 81 N→G 100% intergenic (–/‑151)  / → repA –/replication protein A
JC CP000731 6,552 +28 bp 100% intergenic (+1069/‑419) cadX → / → USA300HOU_pUSA300HOUMR0010 cadmium efflux regulator/MarR family transcriptional regulator
RA CP000731 25,663 Δ1 bp 100% coding (316/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein
RA USA300TCH1516_ALE 110,660 T→A 17.4% I173I (ATT→ATA USA300HOU_RS00515 → putative lipoprotein
RA USA300TCH1516_ALE 111,512 A→G 36.6% S194S (TCA→TCG USA300HOU_RS00520 → putative lipoprotein
RA USA300TCH1516_ALE 111,516 C→A 37.0% Q196K (CAA→AAA)  USA300HOU_RS00520 → putative lipoprotein
RA USA300TCH1516_ALE 512,643 A→G 63.6% intergenic (+711/‑6052) recR → / → speA_2 Recombination protein RecR/Arginine decarboxylase
RA USA300TCH1516_ALE 1,416,458 A→T 100% I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD
RA USA300TCH1516_ALE 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_RS07375 → hypothetical protein
RA USA300TCH1516_ALE 1,646,189 G→C 100% A155G (GCA→GGA)  ypdF ← Aminopeptidase YpdF
RA USA300TCH1516_ALE 1,744,472 G→A 73.6% A67V (GCT→GTT)  apt ← Adenine phosphoribosyltransferase
RA USA300TCH1516_ALE 1,981,418 C→A 77.9% intergenic (‑17/+339) USA300HOU_RS09870 ← / ← liaR hypothetical protein/Transcriptional regulatory protein LiaR
RA USA300TCH1516_ALE 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_RS10125 ← Putative multidrug export ATP‑binding/permease protein
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,372,484 A→T 26.1% L431* (TTA→TAA)  pbuG ← Guanine/hypoxanthine permease PbuG
RA USA300TCH1516_ALE 2,532,298 A→T 100% T15T (ACA→ACT femA_3 → Aminoacyltransferase FemA
RA USA300TCH1516_ALE 2,774,855 A→G 17.8% D777D (GAT→GAC clfB ← Clumping factor B

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ USA300TCH1516_ALE 2176681–2179433 2181135–2179437 5–4455 10 [9] [9] 11 ilvA/USA300TCH1516_02069 L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 512620 =27 (0.840)32 (1.440)
+40 bp
25/180 NT 63.2% intergenic (+688/‑6075) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE 1998278 = NA (NA)intergenic (‑6/+3329) USA300TCH1516_01882/USA300TCH1516_01883 tRNA‑Val/tRNA‑Ala
* ? USA300TCH1516_ALE 848604 =19 (0.590)3 (0.100) 3/244 NT 14.3% intergenic (+609/‑271) trxB/USA300HOU_RS04145 Thioredoxin reductase/Nucleotide‑binding protein
?USA300TCH1516_ALE 848625 = 18 (0.600)intergenic (+630/‑250) trxB/USA300HOU_RS04145 Thioredoxin reductase/Nucleotide‑binding protein
* ? USA300TCH1516_ALE 2085296 =30 (0.930)6 (0.210) 5/232 NT 18.8% coding (173/201 nt) hlb_1 Phospholipase C
?USA300TCH1516_ALE = 2128343 25 (0.870)coding (1/825 nt) hlb_2 Phospholipase C
* ? USA300TCH1516_ALE 2653484 =26 (0.810)3 (0.100) 3/254 NT 11.0% intergenic (+90/‑254) fbp/USA300HOU_RS13650 Fructose‑1,6‑bisphosphatase class 3/hypothetical protein
?USA300TCH1516_ALE 2653543 = 23 (0.730)intergenic (+149/‑195) fbp/USA300HOU_RS13650 Fructose‑1,6‑bisphosphatase class 3/hypothetical protein