breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 46 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_021214449,0560TC40.0% 5.8 / 18.4 20G501G (GGA→GGGTMARI_RS02175methyl‑accepting chemotaxis protein 1
*NC_021214769,6610AG39.3% 89.0 / 126.0 150S296P (TCG→CCG) TMARI_RS03820S41 family peptidase
*NC_021214769,6570GT38.9% 96.8 / 98.0 149A297D (GCC→GAC) ‡TMARI_RS03820S41 family peptidase
*NC_021214769,6560GC37.0% 152.3 / 109.4 149A297A (GCC→GCG) ‡TMARI_RS03820S41 family peptidase
*NC_021214769,6640CT35.3% 163.8 / 91.0 153G295R (GGA→AGA) TMARI_RS03820S41 family peptidase
*NC_021214769,6480GT34.4% 151.1 / 109.3 151S300Y (TCC→TAC) TMARI_RS03820S41 family peptidase
*NC_0212141,478,7981.A34.2% 197.3 / inf 202coding (22/1281 nt)TMARI_RS07485HD domain‑containing protein
*NC_0212141,478,7982.A34.2% 194.0 / inf 202coding (22/1281 nt)TMARI_RS07485HD domain‑containing protein
*NC_0212141,478,7983.A34.2% 192.5 / inf 202coding (22/1281 nt)TMARI_RS07485HD domain‑containing protein
*NC_0212141,478,7984.G34.2% 234.8 / inf 202coding (22/1281 nt)TMARI_RS07485HD domain‑containing protein
*NC_0212141,478,7985.A34.2% 188.4 / inf 202coding (22/1281 nt)TMARI_RS07485HD domain‑containing protein
*NC_0212141,478,7986.A34.2% 188.8 / inf 202coding (22/1281 nt)TMARI_RS07485HD domain‑containing protein
*NC_0212141,478,7987.T34.2% 188.6 / inf 202coding (22/1281 nt)TMARI_RS07485HD domain‑containing protein
*NC_0212141,478,7988.T34.2% 188.7 / inf 202coding (22/1281 nt)TMARI_RS07485HD domain‑containing protein
*NC_0212141,478,7989.T34.2% 189.1 / inf 202coding (22/1281 nt)TMARI_RS07485HD domain‑containing protein
*NC_0212141,478,79810.T34.2% 186.8 / inf 202coding (22/1281 nt)TMARI_RS07485HD domain‑containing protein
*NC_021214769,6600GT33.1% 182.1 / 73.5 152S296* (TCG→TAG) TMARI_RS03820S41 family peptidase
*NC_021214769,6620TC32.7% 209.6 / 73.5 152G295G (GGA→GGGTMARI_RS03820S41 family peptidase
*NC_021214769,6580CT32.2% 148.0 / 80.6 150A297T (GCC→ACC) ‡TMARI_RS03820S41 family peptidase
*NC_021214358,5110GA30.2% 292.1 / 75.1 173intergenic (‑93/‑92)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein

Marginal new junction evidence (lowest skew 10 of 100 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_021214 = 1478798118 (0.370)96 (0.300)
+AAAGAATTTT
70/410 4.0 44.5% coding (22/1281 nt) TMARI_RS07485 HD domain‑containing protein
?NC_021214 1478799 = 127 (0.380)coding (23/1281 nt) TMARI_RS07485 HD domain‑containing protein
* ? NC_021214 = 447895328 (0.980)32 (0.100) 13/428 17.1 8.9% coding (682/1308 nt) TMARI_RS02170 oxidoreductase
?NC_021214 = 448238 341 (1.070)coding (339/1308 nt) TMARI_RS02170 oxidoreductase
* ? NC_021214 = 256338314 (0.950)30 (0.090)
+ATA
13/438 17.4 8.8% coding (1191/1272 nt) TMARI_RS01235 glucose‑1‑phosphate adenylyltransferase
?NC_021214 256339 = 315 (0.940)coding (1192/1272 nt) TMARI_RS01235 glucose‑1‑phosphate adenylyltransferase
* ? NC_021214 = 1805887NA (NA)6 (0.020)
+51 bp
5/348 19.5 1.7% coding (374/390 nt) TMARI_RS09375 IS200/IS605 family transposase
?NC_021214 1806312 = 459 (1.370)coding (635/663 nt) TMARI_RS09380 methyl‑accepting chemotaxis protein
* ? NC_021214 804804 =379 (1.130)7 (0.020) 6/404 20.4 2.0% coding (554/603 nt) TMARI_RS03980 hypothetical protein
?NC_021214 1044289 = 337 (1.120)intergenic (+117/‑73) TMARI_RS05230/TMARI_RS05235 hypothetical protein/transposase
* ? NC_021214 = 1327959365 (1.090)9 (0.030) 7/430 20.5 2.5% coding (417/762 nt) TMARI_RS06635 ABC transporter permease
?NC_021214 = 1328123 364 (1.140)coding (581/762 nt) TMARI_RS06635 ABC transporter permease
* ? NC_021214 1091192 =297 (0.890)7 (0.020) 7/432 20.5 2.3% coding (1596/2961 nt) TMARI_RS05475 glycoside hydrolase
?NC_021214 1091277 = 300 (0.930)coding (1681/2961 nt) TMARI_RS05475 glycoside hydrolase
* ? NC_021214 911818 =339 (1.010)11 (0.030) 7/434 20.6 3.2% coding (416/1131 nt) TMARI_RS04550 1‑deoxy‑D‑xylulose‑5‑phosphate reductoisomerase
?NC_021214 911918 = 345 (1.070)coding (516/1131 nt) TMARI_RS04550 1‑deoxy‑D‑xylulose‑5‑phosphate reductoisomerase
* ? NC_021214 486872 =370 (1.100)7 (0.020) 7/434 20.6 2.2% coding (4667/5073 nt) TMARI_RS02345 DNA‑directed RNA polymerase subunit beta'
?NC_021214 487340 = 278 (0.860)coding (1834/1845 nt) TMARI_RS02350 ABC transporter substrate‑binding protein
* ? NC_021214 = 1501608355 (1.060)8 (0.030) 6/426 21.0 2.4% coding (553/2088 nt) TMARI_RS07670 elongation factor G
?NC_021214 = 1501747 312 (0.980)coding (414/2088 nt) TMARI_RS07670 elongation factor G