Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A27 F999 I0 R1	113	28.0	2125537	97.5%	2072398	48.6

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,891	G→A	Q68* (CAA→TAA)	ilvN ←	acetohydroxy acid synthase I subunit IlvN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,891	0	G	A	100.0%	89.0 / NA	23	Q68* (CAA→TAA)	ilvN	acetohydroxy acid synthase I subunit IlvN
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/12); total (11/12)

ACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCA  >  NC_000913/3850846‑3850936

aCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                            >  1:1885514/1‑49 (MQ=255)

 cTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCtt                                           <  1:1777487/49‑1 (MQ=255)

     ttACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                       <  1:1459082/49‑1 (MQ=255)

     ttACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                       <  1:2031672/49‑1 (MQ=255)

         gCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTg                                   >  1:620226/1‑49 (MQ=255)

             cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTcc                               <  1:294019/49‑1 (MQ=255)

                   cACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACg                          <  1:1500563/48‑1 (MQ=255)

                    aCGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCt                        <  1:190804/49‑1 (MQ=255)

                     cGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTg                       <  1:1372307/49‑1 (MQ=255)

                     cGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTg                       >  1:1118678/1‑49 (MQ=255)

                       aCATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgt                     >  1:94393/1‑49 (MQ=255)

                          tCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtcgt                  >  1:593506/1‑49 (MQ=255)

                              ccAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtcgtc                 <  1:545526/46‑1 (MQ=255)

                               cAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCAtt              >  1:38284/1‑48 (MQ=255)

                               cAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTg             <  1:280626/49‑1 (MQ=255)

                                aGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGa            <  1:1980743/49‑1 (MQ=255)

                                 gCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc           >  1:2047599/1‑49 (MQ=255)

                                  cTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc           >  1:602874/1‑48 (MQ=255)

                                     aTCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg        >  1:1471601/1‑48 (MQ=255)

                                      tCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg        >  1:1144963/1‑47 (MQ=255)

                                        gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    <  1:1249430/49‑1 (MQ=255)

                                         aTTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    >  1:63328/1‑48 (MQ=255)

                                          tttAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCa  <  1:1585059/49‑1 (MQ=255)

ACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCA  >  NC_000913/3850846‑3850936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A