Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A20 F999 I0 R1
|
98 |
154.1 |
11406411 |
97.3% |
11098437 |
49.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,948,805 |
(G)6→7 |
coding (1165/1521 nt) |
yifB ← |
putative magnesium chelatase YifB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,948,799 | 1 | . | G | 99.0%
| 373.1
/ ‑2.8
| 96 | coding (1171/1521 nt) | yifB | putative magnesium chelatase YifB |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (48/47); total (48/48) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 |
Only 100 of 119 total aligned reads displayed.
TTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGA > NC_000913/3948750‑3948843
|
ttAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGcc > 1:3610291/1‑50 (MQ=255)
tAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGcc < 2:5414085/49‑1 (MQ=255)
aaCGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑gg > 2:722578/1‑50 (MQ=255)
aaCGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑g < 2:70410/49‑1 (MQ=255)
aaCGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑g < 1:4542484/49‑1 (MQ=255)
aaCGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑g < 2:4730313/49‑1 (MQ=255)
aCGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑ggg > 1:3733469/1‑50 (MQ=255)
aCGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑gg < 1:3952539/49‑1 (MQ=255)
cGGTGGCGCTGCTTTCTCCCGGCACTACCGGTTTACTCAAAATGCC‑‑gggg > 2:3545061/1‑50 (MQ=255)
gTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑gggggg < 2:1229609/50‑1 (MQ=255)
gTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑gggggg > 2:836507/1‑50 (MQ=255)
tGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑ggggggg > 2:3004245/1‑49 (MQ=255)
tGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑ggggggg > 2:166275/1‑49 (MQ=255)
tGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑ggggggg < 1:4255038/50‑2 (MQ=255)
ggCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑gggggggt > 1:1163785/1‑48 (MQ=255)
gcgcTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑gggggggt < 1:4268562/49‑3 (MQ=255)
ctgctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTa < 2:3832547/50‑1 (MQ=255)
ctgctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTa < 1:757397/50‑1 (MQ=255)
tgctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTaa > 2:3228304/1‑50 (MQ=255)
gctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAAt > 2:958266/1‑50 (MQ=255)
gctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAAt > 2:4827294/1‑50 (MQ=255)
ctTTCTCCCGGCACTTCCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAAt < 2:1316866/49‑1 (MQ=255)
ctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTaa < 1:3618003/48‑1 (MQ=255)
ctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTaa < 2:5173418/48‑1 (MQ=255)
ctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTaa < 1:5562009/48‑1 (MQ=255)
ctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAAt < 1:2520454/49‑1 (MQ=255)
ctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAAt < 2:4792360/49‑1 (MQ=255)
ctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAAt < 1:2006385/49‑1 (MQ=255)
ctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATg < 1:4609130/50‑1 (MQ=255)
ctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATg > 1:3884681/1‑50 (MQ=255)
ctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATg < 1:5670001/50‑1 (MQ=255)
tttCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATgg > 1:2932735/1‑50 (MQ=255)
ttCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATgg > 2:1730736/1‑49 (MQ=255)
ttCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATgg > 2:2261755/1‑49 (MQ=255)
ttCTCCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATgg > 2:2388499/1‑49 (MQ=255)
tctcCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATggg < 2:5280681/49‑1 (MQ=255)
tctcCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATgg < 2:2817360/48‑1 (MQ=255)
tctcCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATgg < 1:3520515/48‑1 (MQ=255)
tctcCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGa > 2:4349782/1‑50 (MQ=255)
ctcCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGAt > 2:1550180/1‑50 (MQ=255)
tcCCGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGAtc > 1:474549/1‑50 (MQ=255)
ccGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGAtctc > 1:2053928/1‑50 (MQ=255)
ccGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGAtc < 1:1054412/48‑1 (MQ=255)
ccGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGAt < 1:2383910/47‑1 (MQ=255)
ccGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGAt < 1:996857/47‑1 (MQ=255)
cGGCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGAtctc > 2:2751969/1‑49 (MQ=255)
ggCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCa < 1:2612490/50‑1 (MQ=255)
gCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTGATGGGATCTCCAg > 2:5116728/1‑50 (MQ=255)
gCACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAg < 1:1758890/50‑1 (MQ=255)
cACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 2:1528742/1‑50 (MQ=255)
cACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt < 2:1988942/50‑1 (MQ=255)
cACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 1:4956219/1‑50 (MQ=255)
cACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 1:5582095/1‑50 (MQ=255)
cACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 1:2675436/1‑50 (MQ=255)
cACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 1:1859364/1‑50 (MQ=255)
cACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt < 2:3113124/50‑1 (MQ=255)
cACTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTACAGt > 2:3327076/1‑50 (MQ=255)
aCTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCCGt > 2:3315990/1‑49 (MQ=255)
aCTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 2:4696100/1‑49 (MQ=255)
aCTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 1:5422432/1‑49 (MQ=255)
aCTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 2:3326516/1‑49 (MQ=255)
cTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 1:2774668/1‑48 (MQ=255)
cTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 1:3951379/1‑48 (MQ=255)
cTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTga < 1:2469804/50‑1 (MQ=255)
cTACCGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTga < 1:5553388/50‑1 (MQ=255)
cTACCGATTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGt > 1:4937391/1‑48 (MQ=38)
ccGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTgagag < 1:1649067/50‑1 (MQ=255)
ccGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTgaga < 1:3907232/49‑1 (MQ=255)
ccGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTgag < 2:2247946/48‑1 (MQ=255)
ccGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTgag < 1:3254609/48‑1 (MQ=255)
cGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTgagaga < 2:222298/50‑1 (MQ=255)
cGTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTga > 2:3723959/1‑46 (MQ=255)
gTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTga > 1:1973662/1‑45 (MQ=255)
gTTTTACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTga > 2:2467364/1‑45 (MQ=255)
ttttACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTga > 1:4945175/1‑44 (MQ=255)
ttttACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTga > 1:1638138/1‑44 (MQ=255)
ttttACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATc < 1:1378866/50‑1 (MQ=255)
tttACTCAAAATGCCG‑GGGGGGTGGGAATGGGATCTCCAGTGAGAGATc > 1:3349125/1‑49 (MQ=255)
ttACTCAAAATGCCGGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATc < 2:1308749/49‑1 (MQ=255)
ttACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCg < 2:4048971/49‑1 (MQ=255)
ttACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCg < 2:4070531/49‑1 (MQ=255)
tACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCg > 1:2755971/1‑48 (MQ=255)
tACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCg > 1:4010523/1‑48 (MQ=255)
tACTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGa < 1:5522362/49‑1 (MQ=255)
tACTCAAAATGCC‑‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGaa < 1:351620/49‑1 (MQ=255)
aCTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGaa < 1:809695/49‑1 (MQ=255)
aCTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGaa < 1:331479/49‑1 (MQ=255)
cTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAg < 2:2668353/49‑1 (MQ=255)
cTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAg < 1:4440934/49‑1 (MQ=255)
cTCAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGc < 1:772152/50‑1 (MQ=255)
cAAAATGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCgg > 1:1146076/1‑50 (MQ=255)
aaaaTGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCgg < 2:1949046/49‑1 (MQ=255)
aaaaTGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCg < 2:1625694/48‑1 (MQ=255)
aaaaTGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGt > 2:5336729/1‑50 (MQ=255)
aaaTGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc > 2:2897127/1‑50 (MQ=255)
aaaTGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc < 1:4508522/50‑1 (MQ=255)
aaTGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc > 2:3522246/1‑49 (MQ=255)
aaTGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc > 2:2134404/1‑49 (MQ=255)
aaTGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc > 2:5565980/1‑49 (MQ=255)
aTGCCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc > 1:4413876/1‑48 (MQ=255)
|
TTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGA > NC_000913/3948750‑3948843
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
