Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F999 I0 R1
|
75 |
85.3 |
6433650 |
96.2% |
6189171 |
49.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,485,906 |
C→A |
A16S (GCC→TCC) |
pepA ← |
aminopeptidase A/I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,485,906 | 0 | C | A | 100.0%
| 297.5
/ NA
| 74 | A16S (GCC→TCC) | pepA | aminopeptidase A/I |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (44/30); total (44/30) |
AATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATG > NC_000913/4485859‑4485952
|
aaTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAAc < 1:1203942/50‑1 (MQ=255)
aTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACt > 1:1701007/1‑50 (MQ=255)
aTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACt > 1:2459303/1‑50 (MQ=255)
tCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTc > 1:2341188/1‑50 (MQ=255)
ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTcc < 1:433797/49‑1 (MQ=255)
ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTcc < 1:1314502/49‑1 (MQ=255)
ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg > 1:4981399/1‑50 (MQ=255)
ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg > 1:3796920/1‑50 (MQ=255)
ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg > 1:2569047/1‑50 (MQ=255)
ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg > 1:5953731/1‑50 (MQ=255)
agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt > 1:5907131/1‑50 (MQ=255)
agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt > 1:2995067/1‑50 (MQ=255)
gaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTg < 1:1905856/50‑1 (MQ=255)
gaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTg < 1:97469/50‑1 (MQ=255)
ggCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtc > 1:4851980/1‑50 (MQ=255)
gCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtct > 1:2251668/1‑50 (MQ=255)
gCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtct < 1:4167044/50‑1 (MQ=255)
gCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtct > 1:1806020/1‑50 (MQ=255)
cGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtctc > 1:4949379/1‑50 (MQ=255)
gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc < 1:2241274/50‑1 (MQ=255)
gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc < 1:5927535/50‑1 (MQ=255)
gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc > 1:5367181/1‑50 (MQ=255)
gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc > 1:5771830/1‑50 (MQ=255)
cGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCgg > 1:4489404/1‑50 (MQ=255)
cGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCgg < 1:4291429/50‑1 (MQ=255)
gTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCggg > 1:1687408/1‑50 (MQ=255)
gTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCggg > 1:836481/1‑50 (MQ=255)
tGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGc < 1:3049959/50‑1 (MQ=255)
tGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGc < 1:5196045/50‑1 (MQ=255)
gTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCt < 1:3036186/49‑1 (MQ=255)
gTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTa > 1:2282604/1‑50 (MQ=255)
ttCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAc > 1:2198594/1‑50 (MQ=255)
ttCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAc < 1:4261476/50‑1 (MQ=255)
tCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc > 1:5107119/1‑50 (MQ=255)
tCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc > 1:2865921/1‑50 (MQ=255)
cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc < 1:5802006/49‑1 (MQ=255)
cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCg > 1:5215359/1‑50 (MQ=255)
gAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGc > 1:4286290/1‑50 (MQ=255)
gAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGc > 1:596459/1‑50 (MQ=255)
aGACGCCCACGGCAATGCAGGAACGCCTCTGTTTCTCCGGGCTACCGCtt < 1:1047225/50‑1 (MQ=255)
aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt > 1:2756787/1‑50 (MQ=255)
aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt < 1:4858715/50‑1 (MQ=255)
gACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt < 1:1064185/50‑1 (MQ=255)
aCGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt < 1:4075978/50‑1 (MQ=255)
aCGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt < 1:6230534/50‑1 (MQ=255)
cGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttttt < 1:3549310/50‑1 (MQ=255)
cGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttttt < 1:576647/50‑1 (MQ=255)
cccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTac < 1:6030930/50‑1 (MQ=255)
ccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTaca > 1:3973695/1‑50 (MQ=255)
cACGACGATGCAGGAACTCCGCTGTTTCTACGGGCTACTGCTTTTTacac > 1:3379073/1‑50 (MQ=38)
acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac > 1:4375855/1‑49 (MQ=255)
acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac > 1:4064174/1‑49 (MQ=255)
cgacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACt > 1:2460293/1‑49 (MQ=255)
gacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTa > 1:4182496/1‑49 (MQ=255)
gacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTa > 1:4066437/1‑49 (MQ=255)
acgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTaaa < 1:5678706/50‑1 (MQ=255)
cgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAAc > 1:4675485/1‑50 (MQ=255)
gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt < 1:1897067/50‑1 (MQ=255)
gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt > 1:1360505/1‑50 (MQ=255)
aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTc < 1:3404592/50‑1 (MQ=255)
aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTc > 1:1286770/1‑50 (MQ=255)
aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTc > 1:4120460/1‑50 (MQ=255)
aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTc > 1:6341348/1‑50 (MQ=255)
tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTcc < 1:876368/50‑1 (MQ=255)
tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTc > 1:987634/1‑49 (MQ=255)
tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTc > 1:2016429/1‑49 (MQ=255)
gCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCa > 1:4307481/1‑50 (MQ=255)
cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt < 1:3435511/50‑1 (MQ=255)
cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt < 1:3687432/50‑1 (MQ=255)
cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt < 1:2891189/50‑1 (MQ=255)
cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt < 1:5264453/50‑1 (MQ=255)
aGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg > 1:2413285/1‑50 (MQ=255)
aGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg > 1:2513169/1‑50 (MQ=255)
aGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg > 1:5308883/1‑50 (MQ=255)
|
AATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATG > NC_000913/4485859‑4485952
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
