Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F999 I0 R1
|
72 |
72.2 |
7896259 |
97.8% |
7722541 |
35.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,485,906 |
C→A |
A16S (GCC→TCC) |
pepA ← |
aminopeptidase A/I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,485,906 | 0 | C | A | 100.0%
| 275.0
/ NA
| 67 | A16S (GCC→TCC) | pepA | aminopeptidase A/I |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (34/33); total (34/33) |
ACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTT > NC_000913/4485874‑4485937
|
aCGTGGTTCGAAGACGCCCACGACGATGCAGGAAc < 1:5196652/35‑1 (MQ=255)
cGTGGTTCGAAGACGCCCACGACGATGCAGGAACt < 1:987210/35‑1 (MQ=255)
gTGGTTCGAAGACGCCCACGACGATGCAGGAACTc < 1:5865814/35‑1 (MQ=255)
gTGGTTCGAAGACGCCCACGACGATGCAGGAACTc > 1:2095813/1‑35 (MQ=255)
gTGGTTCGAAGACGCCCACGACGATGCAGGAACTc > 1:2095734/1‑35 (MQ=255)
gTGGTTCGAAGACGCCCACGACGATGCAGGAACTc > 1:7282586/1‑35 (MQ=255)
tGGTTCGAAGACGCCCACGACGATGCAGGAACTcc > 1:4160418/1‑35 (MQ=255)
tGGTTCGAAGACGCCCACGACGATGCAGGAACTcc > 1:6946281/1‑35 (MQ=255)
gTTCGAAGACGCCCACGACGATGCAGGAACTCCGc > 1:3831566/1‑35 (MQ=255)
gTTCGAAGACGCCCACGACGATGCAGGAACTCCGc > 1:6458267/1‑35 (MQ=39)
cGAAGACGCCCACGACGATGCAGGAACTCCGCTGt > 1:7716470/1‑35 (MQ=255)
cGAAGACGCCCACGACGATGCAGGAACTCCGCTGt > 1:1246081/1‑35 (MQ=255)
gAAGACGCCCACGACGATGCAGGAACTCCGCTGtt < 1:4649681/35‑1 (MQ=39)
gAAGACGCCCACGACGATGCAGGAACTCCGCTGtt < 1:7614586/35‑1 (MQ=255)
aaGACGCCTACGACGATGCAGGAACTCCGCTGttt > 1:5424265/1‑35 (MQ=37)
aaGACGCCCACGACGATGCAGGAACTCCGCTGttt > 1:5424132/1‑35 (MQ=255)
aaGACGCCCACGACGATGCAGGAACTCCGCTGttt > 1:4115199/1‑35 (MQ=255)
aaGACGCCCACGACGATGCAGGAACTCCGCTGttt < 1:4539287/35‑1 (MQ=255)
aaGACGCCCACGACGATGCAGGAACTCCGCTGttt < 1:4589362/35‑1 (MQ=39)
aaGACGCCCACGACGATGCAGGAACTCCGCTGttt > 1:952638/1‑35 (MQ=255)
aaGACGCCCACGACGATGCAGGAACTCCGCTGttt > 1:814371/1‑35 (MQ=255)
aaGACGCCCACGACGATGCAGGAACTCCGCTGttt > 1:804587/1‑35 (MQ=39)
aaGACGCCCACGACGATGCAGGAACTCCGCTGttt > 1:2143935/1‑35 (MQ=255)
aGACGCCCACGACGATGCAGGAACTCCGCTGTTtc > 1:2631999/1‑35 (MQ=255)
aCGCCCACGACGATGCAGGAACTCCGCTGTTtctc > 1:5068006/1‑35 (MQ=255)
aCGCCCACGACGATGCAGGAACTCCGCTGTTtctc < 1:6714441/35‑1 (MQ=255)
aCGCCCACGACGATGCAGGAACTCCGCTGTTtctc < 1:1059316/35‑1 (MQ=39)
aCGCCCACGACGATGCAGGAACTCCGCTGTTtctc > 1:2707826/1‑35 (MQ=39)
cGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc < 1:150463/35‑1 (MQ=39)
gCCCACGACGATGCAGGAACTCCGCTGTTTCTCCg < 1:3854774/35‑1 (MQ=39)
ccccACGACGATGCAGGAACTCCGCTGTTTCTCCg < 1:365039/34‑1 (MQ=39)
cccACGACGATGCAGGAACTCCGCTGTTTCTCCgg > 1:3790545/1‑35 (MQ=255)
cccACGACGATGCAGGAACTCCGCTGTTTCTCCgg < 1:1315285/35‑1 (MQ=39)
cccACGACGATGCAGGAACTCCGCTGTTTCTCCgg > 1:5041195/1‑35 (MQ=255)
ccACGACGATGCAGGAACTCCGCTGTTTCTCCggg < 1:6383432/35‑1 (MQ=39)
cACGACGATGCAGGAACTCCGCTGTTTCTCCGGGc < 1:5915812/35‑1 (MQ=39)
cACGACGATGCAGGAACTCCGCTGTTTCTCCGGGc < 1:4667871/35‑1 (MQ=255)
acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCt > 1:5176680/1‑35 (MQ=255)
acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCt > 1:1600311/1‑35 (MQ=255)
gacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTAc < 1:5789474/35‑1 (MQ=39)
acgaTGCAGGAACTCCGCTGTTTCTCCGGGCTAcc < 1:3334335/35‑1 (MQ=255)
acgaTGCAGGAACTCCGCTGTTTCTCCGGGCTAcc > 1:7539414/1‑35 (MQ=255)
acgaTGCAGGAACTCCGCTGTTTCTCCGGGCTAcc > 1:2966205/1‑35 (MQ=255)
acgaTGCAGGAACTCCGCTGTTTCTCCGGGCTAcc < 1:4486472/35‑1 (MQ=255)
cgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCg > 1:3166498/1‑35 (MQ=255)
aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt > 1:425273/1‑35 (MQ=255)
aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt < 1:1632749/35‑1 (MQ=38)
aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt < 1:2411443/35‑1 (MQ=255)
aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt > 1:1564633/1‑35 (MQ=255)
aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt < 1:3412402/35‑1 (MQ=38)
aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt > 1:2546529/1‑35 (MQ=38)
tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt < 1:3728607/35‑1 (MQ=255)
tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt < 1:7482397/35‑1 (MQ=255)
tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt < 1:976312/35‑1 (MQ=255)
gCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt < 1:6037893/35‑1 (MQ=255)
gCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt < 1:5828001/35‑1 (MQ=38)
gCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt < 1:1805060/35‑1 (MQ=255)
gCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt > 1:4055655/1‑35 (MQ=38)
gCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt < 1:4452585/35‑1 (MQ=255)
gCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt < 1:160904/35‑1 (MQ=38)
cAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt > 1:1023569/1‑35 (MQ=255)
cAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt < 1:2507141/35‑1 (MQ=38)
cAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt < 1:582089/35‑1 (MQ=255)
cAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt > 1:3016159/1‑35 (MQ=38)
cAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt > 1:4230749/1‑35 (MQ=38)
aGGAACTCCGCTGTTTCTCCGGGCTACCGCttttt < 1:5104204/35‑1 (MQ=255)
aGGAACTCCGCTGTTTCTCCGGGCTACCGCttttt > 1:3859761/1‑35 (MQ=255)
|
ACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTT > NC_000913/4485874‑4485937
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
