Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I0 R1 33 46.6 828184 98.8% 818245 269.0

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTA  >  NC_000913/2173077‑2173652
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                 
GCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAG                                                                                                                                                                                                                                                                                    >  M01186:134:000000000‑AAJAW:1:1117:22902:6558/1‑300 (MQ=60)
     GATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCAACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCACAGCAGCAA                                                                                                                                                                                                                                                                              >  M01186:134:000000000‑AAJAW:1:2106:28034:14969/1‑301 (MQ=60)
                 CCACCATACCCCCGGCTTTAAACGCCTCAGAATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGGGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGTAACAGATTTCCACGATGCAAGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCA                                                                                                                                                                                                                                                                    <  M01186:134:000000000‑AAJAW:1:2105:6946:14352/299‑1 (MQ=60)
                    CCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCAAGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAAGTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGACCAAGGC                                                                                                                                                                                                                                                                >  M01186:134:000000000‑AAJAW:1:1112:3365:12356/1‑300 (MQ=60)
                                         CCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGC                                                                                                                                                                                                                                           >  M01186:134:000000000‑AAJAW:1:2118:22360:10279/1‑300 (MQ=60)
                                                                            ATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCAGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGACGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAG                                                                                                                                                                                                       >  M01186:134:000000000‑AAJAW:1:2103:27364:19710/1‑301 (MQ=60)
                                                                                   GCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGC                                                                                                                                                                                                                                                                >  M01186:134:000000000‑AAJAW:1:2103:13103:11464/1‑237 (MQ=60)
                                                                                                     TGATGCTCATAATGATGACAACCGAGATTAAGTTGCGGAACAAATTTCCACGATGCACGGCGACCGCAATCGCAACGAAGAAGCCGATGGTGGCAAGATCGCCTAACTGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATTTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGTAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGAT                                                                                                                                                                              <  M01186:134:000000000‑AAJAW:1:1107:3278:11420/301‑1 (MQ=60)
                                                                                                      GATGCTCATAATGATGACAGCCGAGATTAAGGTGCGAAACTAATATACACGTGCCACCGCGACAGGCAGCGCCACGAAGAAGCCAATGGTGGCAAGATCGCCAAACGGCAACAACTGATTACCCGGCACACAAACAGCAATTAAAATGGTGGGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCAGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGAT                                                                                                                                                                              <  M01186:134:000000000‑AAJAW:1:1106:25513:16711/300‑1 (MQ=60)
                                                                                                              TAATGATGACACCCGAGATTAAGGTGCGGAACAAATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAT                                                                                                                                                                     <  M01186:134:000000000‑AAJAW:1:2110:26079:13253/301‑1 (MQ=60)
                                                                                                                                       GGGGAACAGATTTCCACGATGCAAGGCGACCGCAATCGCCACGAAGGAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACTACTACTAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCAC                                                                                                                                             <  M01186:134:000000000‑AAJAW:1:1107:13962:11236/300‑1 (MQ=60)
                                                                                                                                                                       CAATCGCCACGAAGACGCGGATGGTGGCCAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGTATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACAC                                                                                                             <  M01186:134:000000000‑AAJAW:1:2103:19265:18344/300‑1 (MQ=60)
                                                                                                                                                                                             GGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCGGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGA                                                                                                                                                                                                    <  M01186:134:000000000‑AAJAW:1:1109:28092:16538/191‑1 (MQ=60)
                                                                                                                                                                                                       TCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGCTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACAACGTAACCCGCGAGGATGCCGATAA                                                                            >  M01186:134:000000000‑AAJAW:1:1108:17153:5424/1‑301 (MQ=60)
                                                                                                                                                                                                                              ACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCAC                                                               <  M01186:134:000000000‑AAJAW:1:1104:20969:9072/291‑1 (MQ=60)
                                                                                                                                                                                                                                       ACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGC                                            <  M01186:134:000000000‑AAJAW:1:2105:17919:4769/301‑1 (MQ=60)
                                                                                                                                                                                                                                                       ATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGGTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGGGACAGGCTCGCCAAATGGACCAA                            >  M01186:134:000000000‑AAJAW:1:2115:16958:6589/1‑301 (MQ=60)
                                                                                                                                                                                                                                                           TGAGTGGGATAAAAAGCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCACGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATT                         <  M01186:134:000000000‑AAJAW:1:2104:17230:16501/300‑1 (MQ=60)
                                                                                                                                                                                                                                                               TGGGAAAAAGACCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCGAAGCCGGGTCAAGTCCAATCAGGAACTCCTGACCGCCGAACTGCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCAGGATGGGTTTAATCACCCGTAGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTTACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGC                    <  M01186:134:000000000‑AAJAW:1:1112:11849:3630/301‑1 (MQ=60)
                                                                                                                                                                                                                                                                 GGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGG                                                                                      >  M01186:134:000000000‑AAJAW:1:1108:16085:4531/1‑233 (MQ=60)
                                                                                                                                                                                                                                                                             GGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAAATCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGAGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATAAGCCCCATCACAAAACCCACGGTGACAGGCTCGACAAATGGACCAAATTTGCGCGGAATAAAGTCGG       >  M01186:134:000000000‑AAJAW:1:1111:15355:22837/1‑300 (MQ=60)
                                                                                                                                                                                                                                                                                 TGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCTCGAGTATCCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAACTTGCCCTGAACAACGTCGGCGCCA  >  M01186:134:000000000‑AAJAW:1:1110:17980:10223/1‑301 (MQ=60)
                                                                                                                                                                                                                                                                                           ccaCCGCCGTATGTCCCAGCAGCAACGCCTGACCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCTGCATCTCTGCCGCCGGTTTTACCGCCCGCAGCAATACACCTTTGAG                                                                                                      >  M01186:134:000000000‑AAJAW:1:1109:25305:8499/4‑193 (MQ=60)
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                 
GCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTA  >  NC_000913/2173077‑2173652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑