Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F17 I0 R1 43 55.2 1410130 81.3% 1146435 215.5

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCATTAGCCGCCAGTTGGGTGTGCAAACC‑AATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGG  >  NC_000913/2173122‑2173609
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       
ctgatggcCCGCCAGTTGGGTGTGCAAACA‑AATGGTTTGCGGCGCAACCCACAGGGTGATGCCCATAATGATGACACCCGGAATTAAGGTGGGGACCAGATTACCCGGATGCACGGCGACCGCCATCCCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATAAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTC                                                                                                                                                                                                                                              <  M01186:129:000000000‑A5M8V:1:1107:12428:5378/243‑1 (MQ=60)
   catgtgcccccggttgggggtgcaacacaaaggttgGCGTCGCGGTCCAAAGGGTGATGCTCATAATGATGACACCCGAAATTAAGTGGCGGAACAAATTCCCACGATGCACGGCTGCCCCCATCGCCCCGAAGAAGCCGATGGTGGCAAGATCGCAAAACGGCAGCCCCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCC                                                                                                                                                                                                                                            <  M01186:129:000000000‑A5M8V:1:2101:16704:24471/215‑1 (MQ=60)
         attgtgtataagaaacagaaccaagattgtggtgcgtggcccaaagggggacgtccataagtgagacacccgagataaaggggcggacaggttttccacgtggcccggcaaccgccatgcccacaAAGAAGCCGATGGTGGCAAGATCGCAAAACGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAG                                                                                                                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:1114:19667:16989/125‑1 (MQ=60)
                     GTGCAAACC‑AATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATAAGGCTTGCCGGAACCACCC‑‑CCGTATGTCCCAGCAGAA‑ACGCCGGGTCAA                                                                                                                                                                                                                        >  M01186:129:000000000‑A5M8V:1:2107:20132:25861/1‑251 (MQ=60)
                         AAACCAAATAGGTTGCGGCTCGATCCACGGGGGGATGCTCATGAGGAGGACACCGGGGATGAAGGTGCGAAACAGAGTCCCACGATGCACGGCGACCGCCATGGCCACGAAGAAGCCGATGGTGGCAAGAACCCCAAAGGGCAGCTCCGGATTACCCGGCACACAACCAGCAATTAAACTGGTGAGTGGGATAAAAATCAGGCTTGCCGAT‑‑CCCCCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAATGC                                                                                                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:2111:17681:9008/251‑1 (MQ=60)
                           agtccggggggccgggagatgtgtaaaaggaaccgccaaaaatgaagacccccgaatTTAAGGGGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCC                                                                                                                                                                                                                    <  M01186:129:000000000‑A5M8V:1:1106:20954:10796/193‑1 (MQ=60)
                            CC‑AATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGTCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCACCACCTGATTACCCGGCACACAAAAAGCAATTACAATCGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑AAGCCGGATCAAGTCCAAT                                                                                                                                                                                                                 >  M01186:129:000000000‑A5M8V:1:2112:22720:7415/1‑251 (MQ=60)
                               aatagttggtggcgagaccccgagggtgagccacaaagtgtgcacccccgagagtatggtccggaaaagatttcccggagacagggcacCCGCCCTCGCCACGAAGAACCCGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAACCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCA                                                                                                                                                                                                               <  M01186:129:000000000‑A5M8V:1:1114:11727:10723/162‑1 (MQ=60)
                                    tttggctcggcatcccacgggtagatcctataatgatcacccccgagtttagggggcggaaacGATTTCCCCGATGCCCGGGGACCGCCTTCGCCCGGAAGAACCCGATGGTGCCAAGATCGCCAAACGGCAGCCCCTGATTCCCCGGCACACAACCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAAC                                                                                                                                                                                                          <  M01186:129:000000000‑A5M8V:1:2103:29270:17071/188‑1 (MQ=60)
                                               ATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCAACCA‑‑CAGCCGTATGTCCCAGCAGAA‑ACGCCGGAACAAAGCCAACCCGACACTCCTGACCGC                                                                                                                                                                                                >  M01186:129:000000000‑A5M8V:1:1112:19774:12512/1‑250 (MQ=60)
                                                 CCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGGGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATAAAGGCCAATCAGGAACTCCTGACCGCCGA                                                                                                                                                                                             >  M01186:129:000000000‑A5M8V:1:2102:7759:9836/1‑251 (MQ=60)
                                                                          tggtgactgtatggtgtagtgtgtttttacaagcaaaagcgggcataccagttgctccggagctccgggggctcgaagatgtttaaaagagaCAGCACGTGATCACCCGGCCCACAACGAGCAATAAAAATGGTAAGGGGGATAAAAACCAGCTTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGC                                                                                                                                                                    <  M01186:129:000000000‑A5M8V:1:2108:17366:27896/159‑1 (MQ=60)
                                                                                   ataagggacggaaacagtttaccagcttgacgggcacccccaaccgcaaagaggaggcgcATGGTGGAAAGAACGCCAAACGCCAGCCCCGGATTACCCGGCACAAAACCAGCAATAAAAAGGGTGAGTGGAATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGC                                                                                                                                                           <  M01186:129:000000000‑A5M8V:1:2102:14021:3745/191‑1 (MQ=60)
                                                                                         GTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGAGAGTGGGATAAAAATCAGGCTTGCCGCTCCCA‑‑CCGCCGTATGTCCCAGCAACA‑ACGCCGGATCAAGGCCAATCAAGAACGCCTGACCGCCGAACTTacacagcaaccacctaaacccctgctacacgagggg                                                                                                                                                     >  M01186:129:000000000‑A5M8V:1:1111:7335:15540/1‑215 (MQ=60)
                                                                                                 catgtctccgaGATGCACGGCCACCGCCATCGCCACGAAGCAGCCGATGGTGGAAAGATCGCCAAACGGCTGCACGTGATTCCCCGTCACCAAAACAGCAATTAAAATGGTGAGTGGGATAAAAACCAGCCTTGCCG‑‑ACCCCCCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAC                                                                                                                                             <  M01186:129:000000000‑A5M8V:1:2103:18792:24316/240‑1 (MQ=60)
                                                                                                 aagagtcccacgtggcacgcggaccccaatcccaacgaagaagcatgttgtgaaaagatccccaaagggcaccctgtgattccccggcaacaAAACAGCAATAAAAATGGGGAGTGGGAAAAAAATCGGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAC                                                                                                                                             <  M01186:129:000000000‑A5M8V:1:2104:11088:24988/159‑1 (MQ=60)
                                                                                                    attcccagaagcaacgggacCCGCCATCGCCACGAAGACGCGGATGGTTGCAAGTGCGCCAAACGGCAGCACCTGATTCCCTGACACCAAAACAGCAATTAAAAGGGTGAGTGGGAAAAAAATCAGGCTTGCCAATAC‑‑CCCCGCCCTATGTCCCATCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAC                                                                                                                                             <  M01186:129:000000000‑A5M8V:1:2103:10310:3316/228‑1 (MQ=60)
                                                                                                     TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAAATCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGACTGCTTAGCGATGGGCGTTAAACCAT                                                                                                                                          >  M01186:129:000000000‑A5M8V:1:1105:18591:27281/1‑250 (MQ=60)
                                                                                                             ATGCACGCGGCCCGCCATCGCCAAGAAGAAGCGGGTGGTGACAAGACCGCCAAACGGCAACAGCGTATTACCCGGCACAAAACCAGCAATTAAAATGGTGAGGGGGAAAAAAATGAGGCTGCCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATG                                                                                                                                  <  M01186:129:000000000‑A5M8V:1:1103:12816:15036/250‑1 (MQ=60)
                                                                                                                        ctgcacacatgtgagtgtcggtgttgtgtttgtttgtttcaggaaaaaggcggcaacggggtggcccgggagttcggtgggctcggagatgggtaaaagaaacagGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACC                                                                                                                      <  M01186:129:000000000‑A5M8V:1:1104:14933:9267/146‑1 (MQ=60)
                                                                                                                             cgccccagagaaaagccgagggcggcaagaccgccacgcggcaacacgtgattacccgcccaaaAAACAGCAATTAAAGTGGGGACTGGAAAAAAAAACAGGCCTGCCGATAC‑‑CGCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAAGCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGG                                                                                                                 <  M01186:129:000000000‑A5M8V:1:2110:26377:7241/187‑1 (MQ=60)
                                                                                                                              cggccggaaagaacgcagggggaacagaaccacagaacggcatcactggatgaccgggcacataaacagcagtaaaatgggtgagtgtaaaaaaattaAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGC                                                                                                                <  M01186:129:000000000‑A5M8V:1:1103:25069:17761/153‑1 (MQ=60)
                                                                                                                               cggcacagaaagcccaagtggcaaaagctcgcaaagcggccccccagaattaccggaactcaaaagAGCAATCAAAATGGGGAGGGGGATAAAAATCCGGCTTGCCCATACCA‑‑CCGCTGTATGTCCCAGCAACA‑AGGCCGGATCAAGGCCAGTCAGCATCTCCTGAGCGCCGAACTTCGCCTGTAAACGAGTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCA                                                                                                               <  M01186:129:000000000‑A5M8V:1:2112:20801:7731/185‑1 (MQ=60)
                                                                                                                                      tttgcgagaaagagggggcatgagagttggcgagggtgcgtgcgggcccgggggtgtgatacaagaaacagtggatggttgggataaaaatcgggctggcggacacCCCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGCCCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGC                                                                                                          <  M01186:129:000000000‑A5M8V:1:1114:23225:21136/145‑1 (MQ=60)
                                                                                                                                                                        GCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACGA‑‑CCGCCGTATGTCCCAGCTGCA‑ACGCCGGATCAAGGCCAATCAGGACATCCTGACCGCCGAACTTCGCCTGTAAACGACTACTAGCCTGCTTAACGCTGGGCTTTAACCCATCCATGATGGGTTTAATCACCCGCGGCATTACCAGCATCATTACCGCCCTTTTTACTGCCAGTTGCAAA                                                                      >  M01186:129:000000000‑A5M8V:1:2102:8800:23899/1‑251 (MQ=60)
                                                                                                                                                                            cccggtaacccggtcaccaaaaccaaattgaaaaggggtgtggggaaaaaaaccaggcgtcccgatacCCCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATAC                                                                    <  M01186:129:000000000‑A5M8V:1:2101:10305:25430/183‑1 (MQ=60)
                                                                                                                                                                            CTGATTCCCCGGCACACAACGCGCAATAAAAATGGTGAGTGGGATAAAAATCA‑GCCTGGCGGTACC‑CCCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGACCTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACAC                                                                  <  M01186:129:000000000‑A5M8V:1:1108:13173:8477/251‑1 (MQ=60)
                                                                                                                                                                               tgggggttttgaaaaaaagacagattaaaagtggggagtggaaaaaaaaccggccttgccataccCCCCGCCGTATGTCCCAGCAGCA‑CCGCCGGATCAAGGCCAATCAGGACCTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACC                                                                 <  M01186:129:000000000‑A5M8V:1:2102:20510:26194/186‑1 (MQ=60)
                                                                                                                                                                               ATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCActccacatg                                                               >  M01186:129:000000000‑A5M8V:1:2102:13761:12395/1‑242 (MQ=60)
                                                                                                                                                                                   cccgcccaccaaccacaaataAAAATGGGGAGTGGAAAAAAAACCAGTCTTGCCGATAC‑‑CCCCGCCGTATGCCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGAC                                                           <  M01186:129:000000000‑A5M8V:1:1110:12597:21123/230‑1 (MQ=60)
                                                                                                                                                                                                CAGCAATTAAAATGGTGAGTGGGATAAACATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCCACGAGCTTCCTTACCGATACGCGTTCAACCCTCCTTCATGGGTTTACTCACCCGTGCCCTTACCAGCATCACTGacgcccatttatccgccaccttcacaccacctttgtcaccgtaacccgc                                               >  M01186:129:000000000‑A5M8V:1:1114:24647:11570/1‑201 (MQ=60)
                                                                                                                                                                                                CAGCAATTAAAATGGTGAGGGGGATAAAAATCAGGCTTCCCGAT‑‑CCCCCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGA                                                                                                                                                         <  M01186:129:000000000‑A5M8V:1:1106:26781:14870/144‑1 (MQ=60)
                                                                                                                                                                                                             GGTGAGTGGGAGAAAAACCGGGCTTCCCGATCCCA‑‑CCGCATTATGCCCCAGCAGCC‑ACGCCGGATCAAGGCCAATCAGGATTTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGTTGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGAT                                   <  M01186:129:000000000‑A5M8V:1:2102:10688:21781/249‑1 (MQ=60)
                                                                                                                                                                                                                 ggaggtgaaaaaaactcagctatccaaataACCCCGCCGTATGTCCCAACAGCAGACG‑CGGACCAAGGCCAATCGGGAACTCCCGACCGCCTAACGTCGCCTTTAAACGCATACGGGCCTGCATAGCGATGGGCCTTAAACCATCCATGATGGGTTTAATCACCCGTGGCTTTAGCAGCATCACTTCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAAT                                <  M01186:129:000000000‑A5M8V:1:1110:12503:28685/220‑1 (MQ=60)
                                                                                                                                                                                                                                      GCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACAGCCAGCTGCAATACACCTTTGACATCGTAACCCGCCGGGATGCCGATcatcctgtctcttataccaatctccga        >  M01186:129:000000000‑A5M8V:1:2107:21395:19028/1‑224 (MQ=60)
                                                                                                                                                                                                                                               ccaCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGAATGCCGGTAATCAGCCCCATCCAAAAACCCCCGGGGACAGG  >  M01186:129:000000000‑A5M8V:1:2103:23835:23159/4‑250 (MQ=60)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       
AGCATTAGCCGCCAGTTGGGTGTGCAAACC‑AATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCA‑ACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGG  >  NC_000913/2173122‑2173609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑