Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F17 I0 R1 109 78.2 1797446 84.3% 1515246 230.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGT  >  NC_000913/2173135‑2173603
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
CGTTGGGTGTGCAAACCAATAGTTTGCGTCGCAGTCCCCAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGGGGAACAGATTTCCACGATGCCCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGC                                                                                                                                                                                                                          <  M01186:129:000000000‑A5M8V:1:2101:25639:15143/251‑1 (MQ=60)
AGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGGGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCCGCAGCAACG                                                                                                                                                                                                                           >  M01186:129:000000000‑A5M8V:1:1106:10858:18221/1‑250 (MQ=60)
             AACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTCCCGATACCA‑‑CCGCCGTATGTCCCAGCAcaacccccggtccaaggcca                                                                                                                                                                                                             >  M01186:129:000000000‑A5M8V:1:1112:19407:10121/1‑231 (MQ=60)
                    tgtttgcgtgcgGATCCAAAGGGTGATGCCAATAATGATGCCCCCCGAAATTAGGGTGCGGAACAGATTTCCACGAGGCACGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCAAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGA                                                                                                                                                                                                      <  M01186:129:000000000‑A5M8V:1:1103:18417:12183/239‑1 (MQ=60)
                                   CCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGACCTCCTGACCCCCGA                                                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:1114:14237:2648/1‑251 (MQ=60)
                                   CCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGA                                                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:2104:16995:23906/1‑251 (MQ=60)
                                   CCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGACCACGGCCAATCAGGAACTCCTGACCCCCGA                                                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:2113:25572:16875/1‑251 (MQ=60)
                                   CCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGAATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGA                                                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:1108:25808:18003/1‑251 (MQ=60)
                                        GGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGCAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACATGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTA‑GTCACACCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTA                                                                                                                                                                                  >  M01186:129:000000000‑A5M8V:1:1112:11564:24018/1‑250 (MQ=60)
                                               GCTCATAATGATGACACCCGAGATTAAGGTGCGGAAAAGATTTCCACGATGCATGGCGACCGCCATCGGAACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAATCAGCAATTAAAATGGAGAGAGGGAT‑ACAAATC‑ACGCTTGCCGCTAC‑‑CCCCGCCGTATGTACCAGCAGCAAAGCCGGAGCAAGGCCATTCCGGAACTCCTGACgcccgacctagccaggaa                                                                                                                                                                           >  M01186:129:000000000‑A5M8V:1:1111:16360:29186/1‑233 (MQ=60)
                                                  cataaggatgacccgcgggtttagggtgcggacaagtttcccaataggcagGGCGACCGCCAGCGCCACGAGGAAGCCGATGGTGGAAATATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAC                                                                                                                                                                        <  M01186:129:000000000‑A5M8V:1:1107:10750:5024/200‑1 (MQ=60)
                                                  CATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATAAGGAACctgtcccttataaccatctccgagccc                                                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:2109:11557:13593/1‑223 (MQ=60)
                                                     tatgaacacccacggatatagggggcggaaagatttcccccggagccgggcgaccgcaatcccccaGAAGAAGCCTATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACCAAAACAGCAATTAAAAGGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAC                                                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:1113:14839:2205/185‑1 (MQ=60)
                                                     acggagacccccggaaatgagGGTGCGGAACAGATATCCACGATGCACGGCCCCCGCCATCGCCACGAAGAAGCCAATGGTGGCAAGAACGCCAAACGGCAGCTCCTGATTCCCCGGCACACAAACAGAAATTAAAATGGTGAGTGGAAT‑AAAAAAC‑AGGCTTGCCGATAC‑‑CGCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAC                                                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:2105:20085:20355/230‑1 (MQ=60)
                                                     aagggggacccccgaaagaagggtggggaaaagttttccacgatgaccgggcaccggctcCGCCACGAAGAAGCGAAGGGTGGAAAGATCGCAAAACGGCAGCACCTGATTCCCCGGCCCACAAACAGAAATAAAAATGGTGAGTGGGAT‑AAAAACC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAC                                                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:1110:11001:24162/191‑1 (MQ=60)
                                                            ACCCCCGACATTAAGGTGGGGAACGGTTTCCCACGATGCCCGGCGACCCCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACAAAAACAGCAATTAAAAGGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGC                                                                                                                                                              <  M01186:129:000000000‑A5M8V:1:1108:15838:6989/251‑1 (MQ=60)
                                                               CCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGAATGTCCCAGCAGCAACGCCGGATCAAGGCTAATCAGGAACTCCTGACCGCCGAACTTCGCCcttccccttttagcaatct                                                                                                                                                            >  M01186:129:000000000‑A5M8V:1:2104:10872:17072/1‑231 (MQ=60)
                                                                CCGATATTAGGGCGCGGACCAGATTCCCACGATGCACGGCGACCGCAATCGCCACGAAGAAGCCTGTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGC                                                                                                                                                          <  M01186:129:000000000‑A5M8V:1:1106:16848:28243/251‑1 (MQ=60)
                                                                   AGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCCGCAGCAACGCCGGGTCAAGGCCAATCAGGAACTCCTGACCCTCGAACTTGCACTGTAAACGCCTACGAGCCTCCTTA                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:1102:3369:17093/1‑251 (MQ=60)
                                                                           GTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCGAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTATCGATGGG                                                                                                                                               >  M01186:129:000000000‑A5M8V:1:2105:11685:18999/1‑251 (MQ=60)
                                                                           GTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCCATGGG                                                                                                                                               >  M01186:129:000000000‑A5M8V:1:1104:19905:15261/1‑251 (MQ=60)
                                                                                   cgtgttaccagcaagccgggcacccgccacggccaggaaaaagccgatgggggaaagaccccaaaccggaagccccgtattccccggcaaaaaaaaAGCAATTAAAATGGTGAGTGGGA‑‑AAAAATCAAGGCTTGCCGAT‑‑CCCCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAC                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:1111:6853:18444/155‑1 (MQ=60)
                                                                                   atgatctccgagacgcccggccaccggcatcgcaagaagaaacccgatgggggcaagaccacaAAACGGCATCACGTGATTACCGGGCACACAAACAGCAATTAAAATGGTGAGTGGGAA‑AAAAATG‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAC                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:1111:28475:15238/188‑1 (MQ=60)
                                                                                      ttcccagcgaggcccggcgccccCCATCCCCACGAAGAAGCCGAGGGTCGCAAGATCGCAAAACGGCAGCCACTGATTCCCCGGCCCAAAACCAGCATTTAAAAGGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAC                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:2105:14224:14005/225‑1 (MQ=60)
                                                                                                  ccggacgcccgccaccgcccaGAAGAAGCCGATGGTGGCAAGACCGCAAAACGGCAGCACCTGATTCCCCGGCCCACAAACAGCAATAAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTGGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTT                                                                                                                        <  M01186:129:000000000‑A5M8V:1:1113:18802:20022/230‑1 (MQ=60)
                                                                                                       gaacccCCATCGCCACAAAGAAGCCGATGGTGGCAAGATCGCCAAAGGGCAGCACCTGATTCCCCGTCACACAACCAGCATTTAAAAGGTGGAGGGGGAT‑AAAAATC‑AGGCTTGCCGAAACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAT                                                                                                                    <  M01186:129:000000000‑A5M8V:1:2108:11871:22972/244‑1 (MQ=60)
                                                                                                                          aaaatacgccgtggtgggaagggtcgccaaagggcacgactgaaaccccgggccaacaaaaagaaattaaaagggtgatgtgaataaaaagcagcctggccgataccgCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAACCGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGC                                                                                                    <  M01186:129:000000000‑A5M8V:1:1101:13039:24285/143‑1 (MQ=60)
                                                                                                                                      GGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCG                                                                                    >  M01186:129:000000000‑A5M8V:1:1102:6377:14645/1‑251 (MQ=60)
                                                                                                                                                               tatatgCCCGGTACACAAACAGCAATTAAAATGGTAAG‑GGGATAAAAAACC‑AGGCTGCCCGATAC‑‑ACCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACC                                                           <  M01186:129:000000000‑A5M8V:1:1110:27259:17410/245‑1 (MQ=60)
                                                                                                                                                                GATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGAATAACTGCCGCCGTTTTTACCGCCAGCGGCAATACAC                                                            >  M01186:129:000000000‑A5M8V:1:1109:23294:26851/1‑249 (MQ=60)
                                                                                                                                                                GATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCG                                                                                                                                             >  M01186:129:000000000‑A5M8V:1:1102:29221:14950/1‑168 (MQ=60)
                                                                                                                                                                            CACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTAACGCCAGCTGCAATACACCTTTGACATCGTAA                                              >  M01186:129:000000000‑A5M8V:1:1112:7095:11172/1‑251 (MQ=60)
                                                                                                                                                                                  aaaacagaaataaaaagggtgaggggcaaaaaaaccgggtttgccataccCCCCGCCTTATGTCCCAGCAGCAACGCCGGATCAAGGCAAATCAGGAACCCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACC                                            <  M01186:129:000000000‑A5M8V:1:2104:23501:19460/201‑1 (MQ=60)
                                                                                                                                                                                     CAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCCGCTCCAATACACCTTTGACACCGTAACCCGCCAGG                                     >  M01186:129:000000000‑A5M8V:1:1104:3724:18722/1‑251 (MQ=60)
                                                                                                                                                                                      AATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGA                                    >  M01186:129:000000000‑A5M8V:1:1112:14748:9528/1‑251 (MQ=60)
                                                                                                                                                                                          AAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGC                                 >  M01186:129:000000000‑A5M8V:1:2104:19663:16567/1‑250 (MQ=60)
                                                                                                                                                                                                  GAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCCCGCGGATTCCGATAATC                         >  M01186:129:000000000‑A5M8V:1:1106:27504:21933/1‑250 (MQ=60)
                                                                                                                                                                                                              AAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATAAACCTTTGACATCGTAACCCGCAAAGATGCCGGTAATCAGCCCCATCACA             >  M01186:129:000000000‑A5M8V:1:1114:18886:10668/1‑251 (MQ=60)
                                                                                                                                                                                                                      aggctcaccgatcccaCCGCCGTATGGCCCAGCGGCAAGGCCGGATCAAGGCCAATCAGGAACTCCTGACCGGCGAACTTCGCCTGTAAACGACTACGAGCCTGCTGAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAC         <  M01186:129:000000000‑A5M8V:1:1105:10123:25925/234‑1 (MQ=60)
                                                                                                                                                                                                                          GCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACGGCCAGCTGCCATACACCTTTGACATCGTAACCCGCGAGGATGCCGCTAATCAGCCCCCTCACAAAACCCccggg  >  M01186:129:000000000‑A5M8V:1:1102:15333:8758/1‑246 (MQ=60)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
AGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATC‑AGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGT  >  NC_000913/2173135‑2173603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑