Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F25 I2 R1
|
15 |
37.8 |
1449102 |
96.6% |
1399832 |
125.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| MC JC |
NC_000913 |
4,001,645 |
Δ5 bp |
coding (220‑224/951 nt) |
corA → |
magnesium/nickel/cobalt transporter |
|
| | | | seq id |
start |
end |
size |
←reads |
reads→ |
gene |
description |
|---|
| * |
* |
÷ |
NC_000913 |
4001645 |
4001649 |
5 |
34 [0] |
[0] 36 |
corA |
magnesium/nickel/cobalt transporter |
|
| |
seq id |
position |
reads (cov) |
reads (cov) |
score |
skew |
freq |
annotation |
gene |
product |
| * |
? |
NC_000913 |
= 4001644 | 0 (0.000) | 34 (0.910) |
30/250 |
0.4 |
100% |
coding (219/951 nt) |
corA |
magnesium/nickel/cobalt transporter |
| ? | NC_000913 |
4001650 = |
0 (0.000) | coding (225/951 nt) |
corA |
magnesium/nickel/cobalt transporter |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCG > NC_000913/4001509‑4001778
|
GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTT > NB501016:228:HFK2FAFXY:3:11406:16561:8446/1‑151 (MQ=60)
CAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAG < NB501016:228:HFK2FAFXY:3:11403:25186:13588/122‑1 (MQ=60)
CTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACC > NB501016:228:HFK2FAFXY:2:21207:13368:15799/1‑150 (MQ=60)
GGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGA < NB501016:228:HFK2FAFXY:2:21303:7289:11427/150‑1 (MQ=60)
AGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGT > NB501016:228:HFK2FAFXY:3:21406:2935:9303/1‑151 (MQ=60)
GGCAACCCGCCCGGATCTGGAAGACATCGAAGCATCGGCACGTTTCTTTGTAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGGAGATGCGGAAGATGACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTG < NB501016:228:HFK2FAFXY:4:11405:14426:9384/149‑1 (MQ=60)
GGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCA > NB501016:228:HFK2FAFXY:1:21103:9878:8191/1‑85 (MQ=60)
GAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGA > NB501016:228:HFK2FAFXY:1:21302:25988:14057/1‑69 (MQ=60)
CATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATCGTCGTCTGTTTACTCTGCGTGAGCGTGAAC < NB501016:228:HFK2FAFXY:4:11609:4807:7588/147‑1 (MQ=60)
ACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTA < NB501016:228:HFK2FAFXY:1:11206:25558:13640/151‑1 (MQ=60)
CGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTAT > NB501016:228:HFK2FAFXY:3:21604:7933:18915/1‑151 (MQ=60)
GAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCG < NB501016:228:HFK2FAFXY:3:21508:20964:19925/150‑1 (MQ=60)
GAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCG < NB501016:228:HFK2FAFXY:4:11510:13677:3474/117‑1 (MQ=60)
|
GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCG > NC_000913/4001509‑4001778
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
