Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F35 I0 R1	195	10216.4	8585726	98.3%	8439768	135.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	2,408,861	Δ1 bp	100%	pseudogene (1097/3115 nt)	mdtF →	multidrug efflux pump RND permease MdtF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	2,408,861	0	T	.	99.2%	920.1 / ‑2.9	200	pseudogene (1097/3115 nt)	mdtF	multidrug efflux pump RND permease MdtF
Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (106/92); total (107/93)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 201 total aligned reads displayed.

TGAAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAATTCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCG  >  NZ_CP022686/2408726‑2408996

tGAAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAAtcatc                                                                                                                                     <  8:541506/140‑5 (MQ=255)

     aCGGTTTATCATTAAGCCTCCACACCGTTTATCAAAATTTCTATTCATGAATGTTTCAAAACGCTTGGTGATGCGATCATCCTCGTCTTCCTGTTCATGTATCTGTGTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgac                                                                                                                               <  8:7377/140‑1 (MQ=255)

         tttATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAtt                                                                                                                           >  2:688851/1‑140 (MQ=255)

         tttATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAtt                                                                                                                           >  7:753243/1‑140 (MQ=255)

         tttATCCTTACGACACCACACCGTTTATCAAAATTTCTATACAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAtt                                                                                                                           >  2:761004/1‑140 (MQ=255)

           tATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAtt                                                                                                                           >  6:317156/1‑138 (MQ=255)

            aTCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGcc                                                                                                                        >  1:789634/1‑140 (MQ=255)

                 tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAcc                                                                                                                   >  1:965623/1‑140 (MQ=255)

                 tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTAATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAcc                                                                                                                   <  7:22211/140‑1 (MQ=255)

                  aCGACACCACCCCGTTTATCAAAATATCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTTTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCg                                                                                                                  <  4:557607/140‑1 (MQ=255)

                  aCGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATACCGACGATTGCCTTACCg                                                                                                                  >  2:1028504/1‑140 (MQ=255)

                  aCGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATATGTTTTTGCAGAATTTCCGCGCCACCA‑TCATCCCGTCGATTGCCGTAGCg                                                                                                                  >  1:1097032/1‑140 (MQ=255)

                   cgccaccacaccGTTTATCAAACTTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCgg                                                                                                                 <  8:513105/137‑1 (MQ=255)

                   cGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAcc                                                                                                                   >  5:818470/1‑138 (MQ=255)

                      caccacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCggtgg                                                                                                              <  2:399918/140‑1 (MQ=255)

                       accacaccTTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGATGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCggtggt                                                                                                             <  8:814644/140‑1 (MQ=255)

                       accacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTAGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCggtggt                                                                                                             >  2:899561/1‑140 (MQ=255)

                         cacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAAAGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTa                                                                                                           <  3:1017974/140‑1 (MQ=255)

                           caccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTa                                                                                                           >  6:736798/1‑138 (MQ=255)

                            accGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATtc                                                                                                        >  4:439547/1‑140 (MQ=255)

                                tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTGATGTATCTGTTTTTGCAGAAATTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  1:590908/1‑140 (MQ=255)

                                tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATtctc                                                                                                      >  6:909328/1‑138 (MQ=255)

                                tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  3:505933/1‑140 (MQ=255)

                                tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  1:284097/1‑140 (MQ=255)

                                tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  1:907626/1‑140 (MQ=255)

                                tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTGCCTGGTCAAGTATCTGTTTTTGCATAATATCCGCGCCACAA‑TCATCCCGACGATAGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  1:585921/1‑140 (MQ=255)

                                tttATCAAAAATTCTATTCAGTAAGTTTTCAAAACGCTGGTTGATGCTATCATCCTCGTCTTCCTGGTCAGGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCTTACCGGTGGTTATTCTCgg                                                                                                    >  4:962972/1‑140 (MQ=255)

                                  tATCAAAATTTCTTTTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTACGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  5:47020/1‑138 (MQ=255)

                                  tATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  5:319478/1‑138 (MQ=255)

                                  tATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGa                                                                                                  >  3:1037366/1‑140 (MQ=255)

                                  tATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATACCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  5:554967/1‑138 (MQ=255)

                                  tATCAAAATTTCTATTCAGGAAGTTTGCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGACGTACCGGTGGTTATTCTCgg                                                                                                    <  5:976242/138‑1 (MQ=255)

                                  tATCAAAATTTATATTCAGTAAGTTTTCAAAACGCTGGTTGAGGCAATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGAAGACTTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAAAGGTGGTTATTATCgg                                                                                                    >  5:276343/1‑138 (MQ=255)

                                   aTCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGGCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGAc                                                                                                 >  7:641724/1‑140 (MQ=255)

                                   aTCAAAATTTCTATTCAGGAAGTTTTCAAAACGCAGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTATCGGGAc                                                                                                 >  8:411306/1‑140 (MQ=255)

                                      aaaaTTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACggt                                                                                              >  4:1083139/1‑138 (MQ=255)

                                          tttCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGGCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCg                                                                                          <  4:702153/140‑1 (MQ=255)

                                            tCTATTCAGGCAGTTTTCAAAACGATGGTTGAGTCTATCATCCTCGTCTTCATGGTCATGTAGATGTTTTTGCAGAATTTACGCGCAACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTAGGGCCGTGTGCGag                                                                                        >  8:584764/1‑139 (MQ=255)

                                                 tCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCtt                                                                                     <  5:909328/138‑1 (MQ=255)

                                                       aGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTcggcgg                                                                             <  1:534394/140‑1 (MQ=255)

                                                        gTTTTCCAAACGCTGGTTGAGGCTAGCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGAAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTcggcg                                                                              <  5:727411/138‑1 (MQ=255)

                                                        gTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACTA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGcggt                                                                            <  4:365669/140‑1 (MQ=255)

                                                        gTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAAGTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGcggt                                                                            >  7:784373/1‑140 (MQ=255)

                                                        gTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGGCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGcggt                                                                            <  2:208476/140‑1 (MQ=255)

                                                             cAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgacg                                                                                                                              <  6:421621/85‑1 (MQ=255)

                                                             cAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgacg                                                                                                                              >  5:421621/1‑85 (MQ=255)

                                                               aactgCTGGTTGAGGCTATGATCCTCGTCTTCCTGGTAATGTATCTGTTTTTGTAGAATTTCCGCGCCACAA‑TCATCCCGACGATTTCCGTACCGGTGGTTATTCTCGGGTCGTTTGCGATCTTGTCGGCGGTCGGTTTc                                                                     <  2:124313/136‑1 (MQ=25)

                                                                   gCTGGTTGACGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTATATGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCCGTGGTTATTCACGGGACGTATGCGATCTTGTCGGCGGTCGGATTCACCa                                                                 >  3:953234/1‑140 (MQ=255)

                                                                     tgtttGAGGCTATCATGCTCGTCTTCGTGGTCATGTATCTTTTTTTGCATATTTTCCGCGCCA‑ATTTAATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGAAGTTTGCTATCTTGTCGGCGGTCGGTGTCACAATc                                                               <  8:654281/137‑1 (MQ=255)

                                                                     tGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                               <  1:12626/140‑1 (MQ=255)

                                                                       gtggAGGCTATCATCCGCGTCTTCCTGGTCATGTAGCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCaa                                                             <  8:621689/137‑1 (MQ=255)

                                                                       gTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTTTCTGTTTTTGCAGAATTTCCGCGACACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                               <  5:1042558/138‑1 (MQ=255)

                                                                       gTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCTCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGGCGGTTTCACCATCaa                                                             <  1:1028398/140‑1 (MQ=255)

                                                                       gTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                               >  6:658359/1‑138 (MQ=255)

                                                                       gTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCaa                                                             <  7:441352/140‑1 (MQ=255)

                                                                                 tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTTCCGTACCGGTGGTTATTCTCGGGTCGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   <  8:53036/140‑1 (MQ=255)

                                                                                 tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   >  4:935592/1‑140 (MQ=255)

                                                                                 tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   >  2:36319/1‑140 (MQ=255)

                                                                                 tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   >  3:5915/1‑140 (MQ=255)

                                                                                 tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGACGGTTTCACCATCAACACGTTaaaa                                                   >  7:12667/1‑138 (MQ=255)

                                                                                 tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGGTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   >  7:299553/1‑140 (MQ=255)

                                                                                  catcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACTCGTTAACTa                                                  >  1:71550/1‑140 (MQ=255)

                                                                                   atcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCAACATCAACACGTTAACt                                                   >  5:372658/1‑138 (MQ=255)

                                                                                      cTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGtt                                              >  7:49290/1‑140 (MQ=255)

                                                                                       tCGTCTTCCTGGTCATGTATCTGGTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGAGGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTc                                             >  8:719951/1‑140 (MQ=255)

                                                                                        cGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTCCCGGTGGTTATTCTCGGGACGTTTGCGCTCTTGTCGGCGGTCGGTTTCACCATAAACACGTTAACTATGTTCg                                            >  1:1019836/1‑140 (MQ=255)

                                                                                        cGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCg                                            >  7:370166/1‑140 (MQ=255)

                                                                                           gttCCTGGTCTTGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATGCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  2:713070/139‑1 (MQ=255)

                                                                                           gttCCTGGTCATGTTTCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCTGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  7:962488/139‑1 (MQ=255)

                                                                                           cTTCCTGGTGATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTTCCGGTGGTTATTCTCGGGACGTTTGCGTTCTTGTCGGCGGTCGTTTTAAGAATCAACACGTTAACTATGttgtgta                                         <  8:276623/140‑6 (MQ=255)

                                                                                           cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCgg                                           >  5:646589/1‑138 (MQ=255)

                                                                                           cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  4:239636/140‑1 (MQ=255)

                                                                                           cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  2:308333/140‑1 (MQ=255)

                                                                                           cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  4:520882/140‑1 (MQ=255)

                                                                                           cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  8:880311/140‑1 (MQ=255)

                                                                                             ccccggTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGGCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  6:792811/134‑1 (MQ=255)

                                                                                              ccTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCAGTACAGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACAATCAACACGTTAACTATGTTCTGGATgg                                      >  4:308965/1‑140 (MQ=255)

                                                                                                 ggTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGc                                   <  3:281993/140‑1 (MQ=255)

                                                                                                 ggTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGAGCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGc                                   <  1:508743/140‑1 (MQ=255)

                                                                                                 ggTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGATATTCTCGGGACGTTTGCGATCTTGGCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGc                                   <  8:461317/140‑1 (MQ=255)

                                                                                                 ggTCAGGTATCTGTTTTTGCAGATTATCCGCGCGAGAA‑TCATCCCGTCGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCTATCTGGTCGGCGGTCGGTTTCACCATCATCACGTTATCTATGTTCGGGATGGTGc                                   <  2:83931/140‑1 (MQ=255)

                                                                                                  gTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCt                                  >  8:785702/1‑140 (MQ=255)

                                                                                                  gTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGGGGTTATTCTCGGGACGGTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCt                                  <  1:239729/140‑1 (MQ=255)

                                                                                                     aTGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGc                               >  7:563985/1‑140 (MQ=255)

                                                                                                      tGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCACGGGACGGTTGCGCTCTTGTCGGCGGTCGGTTTCCCCATCAACACTTTAAATATGTTCGGGATGGTGCTGGCg                              >  3:796945/1‑140 (MQ=255)

                                                                                                       gTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGa                             <  7:916758/140‑1 (MQ=255)

                                                                                                       gTATCTGTTTTAGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGTACGTTTCCGATCTTGTCGGCCGTCGGATTCACCAACAACACGTTAACAATGTTCGGGATGGTGCTGGCGa                             >  4:886099/1‑140 (MQ=37)

                                                                                                        tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCg                              <  6:818470/138‑1 (MQ=255)

                                                                                                        tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                            <  2:965623/140‑1 (MQ=255)

                                                                                                        tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                            >  2:903626/1‑140 (MQ=255)

                                                                                                        tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGGCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                            <  8:922068/140‑1 (MQ=255)

                                                                                                        tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATGGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                            <  7:421379/140‑1 (MQ=255)

                                                                                                        aaTCTGTGATTGAAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTTCAGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCAGCATCAACACGTTAACTATGTTCGGGTTGGTGCTGGCGAt                            <  1:814111/139‑1 (MQ=255)

                                                                                                             gTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGt                       <  1:374251/140‑1 (MQ=255)

                                                                                                              gttttGCAGAATTTCCGCTCCAGAA‑TCATCCCGACGTTTTCAGGACCGTTGGTTATTCTCGGGACGTTTGCGAACTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGTTGCTGGCGATAGGGtt                      <  2:535750/139‑1 (MQ=255)

                                                                                                                 ttGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACt                   >  3:889977/1‑140 (MQ=255)

                                                                                                                    aaGAATTTCCGCGACACAA‑TCATCCCGACGATTGCCGTAACGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTAAACTATTTTCGGGATGGTGCTGGAGATAGGGTTACtg                  >  6:702522/2‑138 (MQ=255)

                                                                                                                          ttCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGCCGTTTGCGAACTTGTCGGCGGTCGGTTTCACCATCATCACGATAACTATGTTCGGGAGGGTGCTGGCGATAGGGTTACTGGTGGAt            >  6:193240/1‑138 (MQ=255)

                                                                                                                                  cacaA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCg  <  1:445732/140‑1 (MQ=255)

                                                                                                                                  cacaA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCg  <  8:451586/140‑1 (MQ=255)

TGAAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAATTCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCG  >  NZ_CP022686/2408726‑2408996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A