Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F12 I0 R1 158 5737.7 7407908 98.3% 7281973 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,408,8610T.98.5% 879.9 / 1.9 194pseudogene (1097/3115 nt)mdtFmultidrug efflux pump RND permease MdtF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (80/111);  minor base C (0/2);  total (81/113)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 197 total aligned reads displayed.

CTGAAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAATTCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCG  >  NZ_CP022686/2408725‑2408996
                                                                                                                                        |                                                                                                                                       
cTGAAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATATGTTTTTGCAGAATTTCCGCGCCACAAtcat                                                                                                                                      >  1:145931/1‑137 (MQ=255)
 tGAAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAAtcatc                                                                                                                                     <  5:383228/140‑5 (MQ=255)
      aCGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgac                                                                                                                               <  3:815972/140‑1 (MQ=255)
      aCGGATTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgac                                                                                                                               <  2:517278/140‑1 (MQ=255)
         gTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgacg                                                                                                                              <  7:480802/138‑1 (MQ=255)
         gTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCAGACGAt                                                                                                                            >  3:103783/1‑140 (MQ=255)
          tttATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAtt                                                                                                                           >  2:165593/1‑140 (MQ=255)
             aTCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGcc                                                                                                                        <  6:904937/140‑1 (MQ=255)
               ccTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACTCTGGTTGAGGCTATCATCCTCGTCTTCCTGGGCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGcc                                                                                                                        >  7:66988/1‑138 (MQ=255)
                cTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTa                                                                                                                     <  1:121768/140‑1 (MQ=255)
                attACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCg                                                                                                                       <  7:401527/137‑1 (MQ=255)
                  tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAcc                                                                                                                   <  2:73713/140‑1 (MQ=255)
                  tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGATGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGACGTAcc                                                                                                                   >  5:23250/1‑140 (MQ=255)
                        accacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTACTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCggtggg                                                                                                             >  2:895616/1‑139 (MQ=255)
                        accacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGATATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGAAGAATTTCCTCGCCACAA‑TAATCCCGACGATTGCCGTACCggtggg                                                                                                             >  6:75828/1‑139 (MQ=255)
                         ccacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGtt                                                                                                            >  5:564161/1‑140 (MQ=255)
                         ccacaacGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGTTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGtt                                                                                                            <  4:537849/140‑1 (MQ=255)
                          cacaccGTTAATCAAAATTTCTAAACAGGAAGTTTTCAAAACGCTCGTAGAGTCTATCATCCTCGTCTTCCTCGACATGAATCTGTTAATGCATAAGTACCGCGCCACAA‑TCAACCCGACGAATGCCCTACCTGTGGTTa                                                                                                           >  6:856238/1‑140 (MQ=255)
                             accGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATtc                                                                                                        >  6:42603/1‑140 (MQ=255)
                              ccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATtct                                                                                                       <  4:671953/140‑1 (MQ=255)
                              ccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATtct                                                                                                       <  5:880051/140‑1 (MQ=255)
                                 tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGCCGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  1:834969/1‑140 (MQ=255)
                                 tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCg                                                                                                                  >  6:242471/1‑126 (MQ=255)
                                 tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  4:266056/1‑140 (MQ=255)
                                 tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  3:536038/1‑140 (MQ=255)
                                 tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTAgg                                                                                                    >  5:819828/1‑140 (MQ=255)
                                 tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCAGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAATGCCGTACCGGAGGTTATTCTCgg                                                                                                    >  1:811501/1‑140 (MQ=255)
                                   tATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCggtggt                                                                                                             <  7:91399/129‑1 (MQ=255)
                                   tATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  8:826973/1‑138 (MQ=255)
                                   tATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGAAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCCGTGGTTATTCTAgg                                                                                                    >  7:378241/1‑138 (MQ=255)
                                    aTCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGAc                                                                                                 >  1:6004/1‑140 (MQ=255)
                                    aTCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATAATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGCCGATTGCCGTACCGGTGGTTATTCTCGGGAc                                                                                                 >  6:213086/1‑140 (MQ=255)
                                    aTCAAAATTTCTATTCAGGAAGTTTTCAAAACGATGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGAc                                                                                                 >  4:784624/1‑140 (MQ=255)
                                       aaaaTTTATATTCAGTAAGTTTTCAAAACGATGGTTTAGGCTATAAACCTCTTCTTCCTGGTCATGTATCTGTTTTAGCAGAATTTCCGCGCCACAA‑TCATACCGACGAGTGCCGTAGAGGTTGTTATTATCGGGAGGtt                                                                                              >  2:204040/1‑140 (MQ=255)
                                                         gTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTcggcg                                                                              <  8:312054/138‑1 (MQ=255)
                                                         gTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTAGCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGcggt                                                                            <  3:288250/140‑1 (MQ=255)
                                                          ttttCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGcggtc                                                                           <  3:388412/140‑1 (MQ=255)
                                                            ttCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGcggtcgg                                                                         >  1:556019/1‑140 (MQ=255)
                                                            ttCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGAATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACCTTTCCGATCTTGTCGgcgggcgg                                                                         >  1:115774/1‑140 (MQ=255)
                                                             tCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGcggtcg                                                                          >  7:190080/1‑138 (MQ=255)
                                                                      tGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                               <  3:891616/140‑1 (MQ=255)
                                                                        gTTGAGGCTATCATCCTCGTGTTCCTGGTCTTGTTTCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCaa                                                             <  6:512938/140‑1 (MQ=37)
                                                                        gTTGAGGCTATCATCCTCGTCTTCGTGGTGATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCAtg                                                               <  7:622237/138‑2 (MQ=255)
                                                                        gTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCATAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCaa                                                             <  3:212580/140‑1 (MQ=255)
                                                                        gTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGTCGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                               <  8:392784/138‑1 (MQ=255)
                                                                        gTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCaa                                                             <  2:311572/140‑1 (MQ=255)
                                                                        gTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTATTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCaa                                                             <  5:60174/140‑1 (MQ=255)
                                                                              gCTATCATCCTCGTCTTCCTTGTCATGTATCTGTTCTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGtt                                                       <  5:150941/140‑1 (MQ=255)
                                                                                tagaatcCTCGTCTTCCTGGTAATTTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGATTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTaa                                                     <  5:753784/136‑1 (MQ=255)
                                                                                  tcatcaTCGTCTTACTTGTCATGGATCTGGTTTTGCAGACTTTCCGCGCCCCAA‑TCATCCCGACGATTGCCGTACCTGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGGCGGTTGCACCAGCAACACGTTaa                                                     <  8:735109/138‑1 (MQ=255)
                                                                                  tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGt                                                        <  4:100606/135‑1 (MQ=255)
                                                                                  tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTaa                                                     <  7:587138/138‑1 (MQ=255)
                                                                                  tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   <  6:119997/140‑1 (MQ=255)
                                                                                  tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   <  4:18853/140‑1 (MQ=255)
                                                                                  tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGGGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   <  5:495175/140‑1 (MQ=255)
                                                                                  tcatcCTCGTCTTCCTGGTCAAGGATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAGTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTaa                                                     <  8:86200/138‑1 (MQ=255)
                                                                                    atcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGGCGATTGACGTACCGGTGGGTATTCTCGGGACGTTTGCGAAATTGTCGGCGGTCGTGTTCACAAGAAACACGCTAACt                                                   >  8:225022/1‑138 (MQ=255)
                                                                                    atcCTCGTCTTCCTGGTAATGTATCTGTTTATGCAGAATATCCGCGCCACAA‑TCATCCCGAAGATTGCCGTACCGGTGGTTATTCTCGGGTCGTTTGCGATCTTGTCGGCGGTCGGTTTCACCTTCAACACGTTAACTAt                                                 <  6:549293/140‑1 (MQ=37)
                                                                                    atcCTCGTCATCCTGTGCATGTATCTGTTTTTGCAGAATTTCCGAGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   <  8:522565/138‑1 (MQ=255)
                                                                                       cTCGTCTTCCTTGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGtt                                              <  1:272775/140‑1 (MQ=255)
                                                                                       cTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCTGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGtt                                              >  6:63699/1‑140 (MQ=255)
                                                                                       cTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCTGCGGTCGGTTTCACCATCAACACGTTATCTATGtt                                              <  4:483900/140‑1 (MQ=255)
                                                                                          gTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTc                                             >  7:326308/1‑138 (MQ=255)
                                                                                          gTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTc                                             >  7:775290/1‑138 (MQ=255)
                                                                                          gTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATACCGACGATTGCCGTACCGGTGGTTATTCTCGGGACTTTTGCGATCTTGTCGGCGGTCGGTTTCACAATCAACACGTTAAATATGTTCgg                                           >  1:326780/1‑140 (MQ=255)
                                                                                           tCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCggg                                          <  4:502189/140‑1 (MQ=255)
                                                                                            cttcgaggTCATGTATCTGTTTTTGGAGAATTTCCGCGCCTCAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  2:598898/134‑1 (MQ=255)
                                                                                            cTTCCTGGTCATGTCTCTGTTTTTGCAGAATTTCCGCGACACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  6:648590/140‑1 (MQ=255)
                                                                                            cTTCCTGGTCATGTATCTGTTTTTGCATAATTTCCGCCCCACAA‑GCATCCCGAAGATTGCCGTAGCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  6:528414/140‑1 (MQ=255)
                                                                                            cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  3:124499/140‑1 (MQ=255)
                                                                                            cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGGCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  2:239779/140‑1 (MQ=255)
                                                                                               ccTGGTCATGTATCTGTTTTTGCAGAATTTCCGAGCCACAA‑TCATCCCGACGATAGCCGTACCGGTGGTTATTATCGGGACGTTTGCGATCTTGTCTGCGGTCGGTTTCACCATCAACACGATAACTATGTTCGGGATgg                                      >  3:252761/1‑140 (MQ=255)
                                                                                                 tGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGCGTGCCGTACCGGTGGTTATTCGCGGGCCGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATgg                                      >  8:62183/1‑138 (MQ=255)
                                                                                                 tGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTg                                    <  5:616894/140‑1 (MQ=255)
                                                                                                  ggTCATGTATCTGTTTTTTCTGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGt                                     <  8:136421/138‑1 (MQ=255)
                                                                                                  ggTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGGTATTCTCTGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATTGTGc                                   <  2:653046/140‑1 (MQ=255)
                                                                                                       tGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCg                              >  6:476030/1‑140 (MQ=255)
                                                                                                        gTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGc                               >  8:483170/1‑138 (MQ=255)
                                                                                                        gTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGa                             >  2:92506/1‑140 (MQ=255)
                                                                                                        gTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGa                             <  2:799477/140‑1 (MQ=255)
                                                                                                         tataTGTTTTTGCTGAATTTCCGCGCCACAA‑TGATACCGACGATTGCCTTACCGGTGGTTAATCTGGGGACGTTTTCGATCTTGTCGGCGGTCGGTTTCTCCATCAACTCGTTAACTATGTTCGGGATGGTGCTGGCTAt                            <  2:880691/140‑1 (MQ=255)
                                                                                                         tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCg                              <  8:330471/138‑1 (MQ=255)
                                                                                                         tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCg                              <  7:79407/138‑1 (MQ=255)
                                                                                                         tATCTGTTTTTCCATAATTTCCCCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGATATTCTCGGGACGTTTGCGATCTTGTCGGAGGTCGGTTTCACCATCAACACGATAACTTTGTTCGGGATGGTGCTGGCGAt                            <  5:82481/140‑1 (MQ=255)
                                                                                                          aTCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGGGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGa                             >  8:584199/1‑138 (MQ=255)
                                                                                                              gTTTTTGCAGAATTTCCGCGCCCCAA‑TCCTCCAGACGATTGCCGTACCGGTTGTTATTCTCGGGACGTTTGGGATCTTGTCGGCGGTCGGTTTCACAATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGt                       <  6:615344/140‑1 (MQ=255)
                                                                                                              gTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGt                       <  6:570906/140‑1 (MQ=255)
                                                                                                              gTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATAGCCTGACCGGTGGTTATTCTCGGGACGTTTGTGATCTTGTCGGCGGTCGGTTTCTCCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGt                       <  3:16640/140‑1 (MQ=255)
                                                                                                              gTTTTTGCAGAATTTCCGCGCCAC‑ACTCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAgg                         <  7:257220/138‑1 (MQ=255)
                                                                                                                ttttGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTa                     >  1:717066/1‑140 (MQ=255)
                                                                                                                 tttGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTAc                    >  1:933641/1‑140 (MQ=255)
                                                                                                                   tGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACtg                  >  1:912578/1‑140 (MQ=255)
                                                                                                                         aTTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGAt            >  3:358653/1‑140 (MQ=255)
                                                                                                                           ttCCGCGCCACAA‑TCATCCCGACGATTGCTGTACCGGGGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTGCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGa          <  3:697184/140‑1 (MQ=255)
                                                                                                                           ttCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCACCACGTTAACTAGGTTCGGGATGGTGCTGGCGATAGGGTTACtggtggtt            >  8:810961/1‑136 (MQ=255)
                                                                                                                           ttCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGAGCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGAt            <  7:812846/138‑1 (MQ=255)
                                                                                                                              cgcgCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGAGCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGc       <  6:786857/140‑1 (MQ=255)
                                                                                                                                 gcCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCAt    <  1:539152/140‑1 (MQ=255)
                                                                                                                                 gcCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCAt    <  4:386191/140‑1 (MQ=255)
                                                                                                                                   cacaA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCg  <  4:878776/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CTGAAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAATTCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCG  >  NZ_CP022686/2408725‑2408996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A