Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F2 I0 R1 122 5985.5 7364544 98.3% 7239346 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,839,687 T→G 9.7% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,839,6870TG 9.7% 241.4 / 4.2 132N144T (AAC→ACC) minDseptum site‑determining protein MinD
Reads supporting (aligned to +/- strand):  ref base T (47/69);  new base G (12/4);  total (59/73)
Fisher's exact test for biased strand distribution p-value = 1.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

TCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGT  >  NZ_CP022686/4839548‑4839826
                                                                                                                                            |                                                                                                                                           
tctGCTTACGCGGCGTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGt                                                                                                                                             <  2:943167/140‑1 (MQ=255)
tctGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGt                                                                                                                                             <  4:440502/140‑1 (MQ=255)
tctGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGt                                                                                                                                             <  1:927002/140‑1 (MQ=255)
 ctGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCTGAGTCGCGTACTGAGGAGACTT‑GCGGGtt                                                                                                                                            >  7:181086/1‑140 (MQ=255)
    cagactcgGCATGGGTTCTAGCGCGTTAACAGTAGTTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCTGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTtggt                                                                                                                                         <  7:553361/134‑1 (MQ=255)
    cTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCGATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGGCTT‑GCGGggtggt                                                                                                                                         >  7:652062/1‑140 (MQ=255)
    cTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGCATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGGCTT‑CCtggttggt                                                                                                                                         >  8:489015/1‑140 (MQ=255)
    cTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTtggt                                                                                                                                         <  4:752633/140‑1 (MQ=255)
    cTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACTGAGGAGACTT‑CCGGGTtggg                                                                                                                                         >  3:581786/1‑139 (MQ=255)
    cTTACGCGGCCTGGGTTATAGCGCGGTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGTTCAGAGTCGCGTACTGAGGAGACTT‑CCtggttggt                                                                                                                                         <  4:287888/140‑1 (MQ=255)
     ttACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTtggtg                                                                                                                                        <  8:210986/140‑1 (MQ=255)
     ttACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTtgg                                                                                                                                          <  5:135778/138‑1 (MQ=255)
       aCGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCGCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTggtggt                                                                                                                                      <  4:868552/140‑1 (MQ=255)
         gcgGCCTGGGTTATAGCGCGTTAACAGTATGGGCTCATTAATCGGCTCTTCTCCATTTTCTGCGCGGCGTGATTTAGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCTGACGGGTGAGTCTT‑GCAGggtggtggtgt                                                                                                                                    >  8:221482/1‑138 (MQ=255)
         gcgGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGGGACTT‑ACGGggtggtggtga                                                                                                                                    >  7:734441/1‑138 (MQ=255)
         gcgGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTAATTAGGATACTT‑GCGGGTTGGTGGtaa                                                                                                                                    >  4:125783/1‑140 (MQ=255)
           ggCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTCATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGtaata                                                                                                                                  <  4:229463/140‑1 (MQ=255)
           ggCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGCGGAGACGT‑CCGGGTGTGTGGgaa                                                                                                                                    >  5:569680/1‑138 (MQ=255)
           ggCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTtgg                                                                                                                                          <  6:282526/132‑1 (MQ=255)
           ggCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTtgg                                                                                                                                          >  5:282526/1‑132 (MQ=255)
              cTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CAGGGTTGGTGGttataa                                                                                                                                 >  6:842657/1‑138 (MQ=255)
                   ttATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTc                                                                                                                          <  3:534082/140‑1 (MQ=255)
                     atctcgcgTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGt                                                                                                                        <  8:800203/136‑1 (MQ=255)
                     ataGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTc                                                                                                                          <  5:293132/138‑1 (MQ=255)
                     ataGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTc                                                                                                                          <  5:176429/138‑1 (MQ=255)
                     ataGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGt                                                                                                                        <  1:30281/140‑1 (MQ=255)
                     ataGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATATTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTc                                                                                                                          <  5:570840/138‑1 (MQ=255)
                     ataGCGCGTTAACAGTAGGTGCGCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGt                                                                                                                        <  7:876196/140‑1 (MQ=255)
                     ataGCGCGTTAAAAGTATGTGCTCTTTAATCGGCGCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATCCGGTCAGAGGCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGt                                                                                                                        <  7:872041/140‑1 (MQ=255)
                       aGCGCGTTAACAGTAGGTGCTCTTTACTCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGATTGCCTAAAAGACGGTCAGAGTCGCGTACTGAGGTGCGTTGCGGGGTTGGGGGTATTAA‑GGCttaggcg                                                                                                                      >  7:47040/1‑135 (MQ=255)
                       aGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGTCTTGATTTCGACGCCAGAATGCCTAAAATAAGGTCAGAGTCGCGTACTGAGGAGACTT‑ACGGGTTGGTGGTTATAATGGCTTCGTcg                                                                                                                      >  2:63062/1‑139 (MQ=255)
                       aGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACGGAGGAGCCAT‑CCGGGTAGGTGGTAATAATGGGttgttag                                                                                                                      >  4:491045/1‑135 (MQ=255)
                          gcgTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTg                                                                                                                     <  5:815526/138‑1 (MQ=255)
                          gcgTTAACAGTAGGTGCTCTTTAATCGGCGCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTg                                                                                                                     <  5:715337/138‑1 (MQ=255)
                           cgTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTTCTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCGTCGTATGCaa                                                                                                                  >  2:947777/1‑140 (MQ=255)
                           cgTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGACAGAATGCCTAAAATACGGTCAGAGTCGCGTTCAGAGGAGACTT‑CCGGGTTTGTGGTTATAAGGGCTTAGTCTgaga                                                                                                                  >  4:369283/1‑137 (MQ=255)
                            gTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACTGAGGAGACTT‑ACGGGTTGGTGGTAATAATGGGTTGGGCTGaaaa                                                                                                                 >  1:620558/1‑140 (MQ=255)
                            gTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATCACTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCaaa                                                                                                                 >  2:183218/1‑140 (MQ=255)
                             ttAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGTGACAT‑GCGGGTTGGTGGGTATAATTGGTTCGTCTTCaa                                                                                                                  >  5:770525/1‑138 (MQ=255)
                             ttAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTTGTGGTAATAATTGCTTCGTGTGCaa                                                                                                                  >  6:77370/1‑138 (MQ=255)
                             ttAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTAATGAGGAGACAT‑CCGGGTTGGTGGTAATAATGGCTTAGTATGCaa                                                                                                                  >  6:113743/1‑138 (MQ=255)
                             ttAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACTGTGGTGGCTT‑CCGGGGTGGTGGTTATAATGGCGTGGGCTGCaaaa                                                                                                                >  7:834666/1‑140 (MQ=255)
                                 cAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATaga                                                                                                            <  1:373528/140‑1 (MQ=255)
                                   gTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTAATGAGGGTACTT‑ACGGGTTGGTGGTTATAATGGGTTAGTCTGCTAAATATAGt                                                                                                          >  1:724352/1‑140 (MQ=255)
                                      ggTGCTCTTTAATCGGATCTTCGCCATTTTCTGCGCGGCGTGAGTTCGACGCCAGAATGCCTAAAATACGGTCATACTCGCGGACAGAGGAGCCAT‑GCGGGGTTGTGGTAATAATGTCATCGGCTTCAACAAAGAGTGcc                                                                                                       >  8:390455/1‑140 (MQ=255)
                                          ctctTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGTCTGAGGAGGCAT‑GCGGGTTGGTGGTTATAATGGGTTGGTCTGCAAAATAGAGTGGCATTa                                                                                                   >  1:808814/1‑140 (MQ=255)
                                            ctTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGCCGT‑ACGGGTTGGTGGGTATAATGGCTTCGTGTGGAAAATAGAGTGGCATTaaa                                                                                                 >  4:626904/1‑139 (MQ=255)
                                            ctTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACGGAGGAGCCTG‑ACGGGGTGGGGGTAAAAATGGCGTCGTCTGCAAAATAGAGGGCCAGTaaa                                                                                                 >  2:497283/1‑139 (MQ=255)
                                              ttAATCGGCTCTTCGCCATGTTCTGCGCGGCGTGATTTCGACGCCAGCATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACGT‑CCGGGGTGGTGGGAATAAGTGCTTCGTCGGCAAAATATCGTTCCATGAAc                                                                                                 >  6:830245/1‑138 (MQ=255)
                                                aaTCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCAc                                                                                             <  7:705463/140‑1 (MQ=255)
                                                aaTCGGCTCTTCGCCATTTTCTGCGCGGCGTGAATTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACTGAGGAGACAT‑CCGGGGTGGTGGTAATAATGGCTTCGGGTGCAAAATAGAGTGCCCTTAACGc                                                                                               >  6:804339/1‑138 (MQ=255)
                                                     gCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTAAGGAGGAGACTT‑CCGGGTTTGTGGTAATAATGGCGTCGTATGCAAAATAAATTGACAATAACACACCGCtt                                                                                        >  8:41843/1‑140 (MQ=255)
                                                      ctctTCGCCATTTTCTGCGCGGCGTGAGTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGTCTGAAGAGACAT‑CCGGGTTGGTGGTAATAATGGCGTCGTCTGCAAATAACAGTGCCAATAAACCACCgggat                                                                                       >  1:545195/1‑137 (MQ=255)
                                                         ttCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTTCTGAGGAGACTT‑AAGGGGTGGTGGTTATAATGTGTTTGTGTTGCAATTTGCGTGCCATTAACGCACCGGTTTCaa                                                                                    >  1:492735/1‑140 (MQ=255)
                                                          tCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAAt                                                                                   <  3:924717/140‑1 (MQ=255)
                                                          tCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAAt                                                                                   <  1:412457/140‑1 (MQ=255)
                                                                            cgTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTcaca                                                                 <  2:582098/140‑1 (MQ=255)
                                                                            cgTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTcaca                                                                 <  1:475068/140‑1 (MQ=255)
                                                                                  ttCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGAt                                                           <  2:453921/140‑1 (MQ=255)
                                                                                                gCCTAATATACTGTCAGATTCGCTTACTGAGGAGACTT‑CCTGGTGGGTGTTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATACCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCa                                             <  8:520566/140‑1 (MQ=255)
                                                                                                gCCTAAATTCCGGTCAGAGGCGCGTACTGAGGAGACGT‑CCGGGTTGGTGGTGATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTGCACTCCCTGCCGGGGAGTCACAAACGATAAATTCAACATCCa                                             <  2:678265/140‑1 (MQ=255)
                                                                                                gCCTAAAATACGGTCCGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATc                                               <  6:50220/138‑1 (MQ=255)
                                                                                                gCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATc                                               <  6:162522/138‑1 (MQ=255)
                                                                                                gCCTAAAATACGGTCAGAGTCGCGGACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGGCTGCAAAATAGAGTGCCATTAACGCACAGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATc                                               <  6:83776/138‑1 (MQ=255)
                                                                                                 ccTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCAt                                            <  3:616732/140‑1 (MQ=255)
                                                                                                           ggTCAGAGTCGCGTACTGAGGGGACGT‑CCGGGTTGGTGGTTATAATGGCTTCGTGTGCAAAATAGAGTGGCATTAACGCACCCGTTTCAATCCCAGCCGGGGCGTCACAAACGATAAATTAAAAATCCATCGCGTTgaga                                  >  1:843304/1‑140 (MQ=255)
                                                                                                           ggTCAGAGTCGCGTACTGAGGATACTT‑AAGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTTACGCACCGGGTTCAATCCCAGCCCGGGAGTCACCAACGCAAAAATCAAAATCCATCGACTTCAgg                                  >  4:402821/1‑139 (MQ=255)
                                                                                                           ggTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGa                                  <  1:200510/140‑1 (MQ=255)
                                                                                                           ggTCAGAGTCGCGTACAGAGGAGACTT‑ACGGGGTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTTCCAATAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAAAGATAAATTAAAAAACCATCGCTTTCAGa                                  >  2:179817/1‑140 (MQ=255)
                                                                                                           ggTCAGAGTAGCGTACTGAGGAGTCTT‑CCGGGTTGTTTGTAATTATTGCTTGGTCTGCTATATAGAGGGCCATTAACGCAGCGGTTTCATTCCCTGCCGGGGAGTCACAAACGATTTATTCAAAATCGATCGCTTTgaga                                  <  2:634747/140‑1 (MQ=255)
                                                                                                                gagTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGatcatc                             <  7:405312/140‑1 (MQ=255)
                                                                                                                      cgttcggaggagACTT‑CAGGGTTTGTGGTTATAATTGCGTAGTCTGCAAAATAGATTGCAATTAAAGCACCGGTTTAAATACCTGCCGGGGAGTCACAAAATATAAATTCAAAATACATAGCTTTAATATAATCAAGaaa                       >  4:13813/7‑139 (MQ=255)
                                                                                                                            gaggagACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGc                 <  1:808773/140‑1 (MQ=255)
                                                                                                                            gaggagACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGc                 <  7:309732/140‑1 (MQ=255)
                                                                                                                             tggagACTT‑CCGGGTGGGTGGTAATTATGTCTTCGTCTGCAAAATAGAGTGCCTTTACCGCACCGGTTTCAATACCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTgg                  <  6:653971/137‑1 (MQ=255)
                                                                                                                             atgataCTT‑CCGGGTTGGTGGTAATAATGGCGTCGGCCTCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCg                <  7:540228/135‑1 (MQ=255)
                                                                                                                             aggagACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAAAAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCAGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGcc                >  7:304091/1‑139 (MQ=255)
                                                                                                                               gcgaCTT‑CCGGGTTGGTGGTAATACTGGCTTCGTCTGCAAAAGAGAGTGCCATTAACGCCCCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAACATCCATCGCTTTCCGATCATCAAGAACTTTGGCg                <  5:190917/136‑1 (MQ=255)
                                                                                                                               gagaCTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCACTCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAc              <  8:809707/140‑1 (MQ=255)
                                                                                                                               gagaCTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCATATCATCAAGAACTTTGGCGAc              <  1:183218/140‑1 (MQ=255)
                                                                                                                               gagaCTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAc              <  4:770173/140‑1 (MQ=255)
                                                                                                                               gagaCTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAc              <  2:7480/140‑1 (MQ=255)
                                                                                                                               gagaCTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAc              <  3:707914/140‑1 (MQ=255)
                                                                                                                               gagaCTT‑CCGGGTTGGTGGTAATAATGGCTGCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAc              <  3:938937/140‑1 (MQ=255)
                                                                                                                                  aCTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCACTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAcccc           >  1:257871/1‑140 (MQ=255)
                                                                                                                                    tt‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAccc            <  4:606437/137‑1 (MQ=255)
                                                                                                                                    tt‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAccc            >  3:606437/1‑137 (MQ=255)
                                                                                                                                    tt‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCtt         >  4:314373/1‑140 (MQ=255)
                                                                                                                                    tt‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTATGGCGAcccc           >  5:664376/1‑138 (MQ=255)
                                                                                                                                       gcGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGCTTCAATACCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCtt         <  5:520366/137‑1 (MQ=255)
                                                                                                                                       ccGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCa       <  1:396622/140‑1 (MQ=255)
                                                                                                                                       ccGGGTTGGTGGTAATAATGGCCTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCtt         <  5:842657/138‑1 (MQ=255)
                                                                                                                                       ccGGGGTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACTATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCtt         <  5:545537/138‑1 (MQ=255)
                                                                                                                                        cGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGACCTTTGGCGACCCCTTCAc      >  2:7024/1‑140 (MQ=255)
                                                                                                                                        cGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCAc      >  2:289846/1‑140 (MQ=255)
                                                                                                                                           gTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCt                                        <  7:194801/103‑1 (MQ=255)
                                                                                                                                           gTTGGTGGTAATAATGGCTTAGGCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCGTTCAGATCATCAAGAACTTTGGCGACCCCTTCACggg   <  7:800001/140‑1 (MQ=255)
                                                                                                                                            ttGGTGGTAATAATTGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGt  <  4:886696/140‑1 (MQ=255)
                                                                                                                                            ttGGTGGTAATAATGCCTTCTTCTGCAAAATAGAGTGCCATTAACGCACCGGGTTCAATCCCAGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACgg    <  6:333889/138‑1 (MQ=255)
                                                                                                                                            ttGGAGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGt  <  3:639621/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTT‑CCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGT  >  NZ_CP022686/4839548‑4839826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A