Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F13 I0 R1 150 9496.0 7621532 98.4% 7499587 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,839,687 T→G 11.4% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,839,6870TG11.4% 233.6 / 5.3 137N144T (AAC→ACC) minDseptum site‑determining protein MinD
Reads supporting (aligned to +/- strand):  ref base T (62/56);  new base G (16/3);  total (78/59)
Fisher's exact test for biased strand distribution p-value = 1.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

CTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGT  >  NZ_CP022686/4839549‑4839826
                                                                                                                                          |                                                                                                                                            
ctGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGCAGCCAGCATGCCTAAAATACGGTCAGAGTCGCGGACAGCTGAGTCGTACGGGtt                                                                                                                                             >  1:477101/1‑140 (MQ=255)
ctGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATCCGGTCAGAGTCGCGTTCTGAGGGGAAGTGCGGGtt                                                                                                                                             >  8:530531/1‑140 (MQ=255)
ctGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTGCGGGtt                                                                                                                                             >  8:219720/1‑140 (MQ=255)
   cTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACTGAGGGGACTTCCGGGTtgg‑t                                                                                                                                         >  5:582159/1‑140 (MQ=255)
    ttACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTtgg‑tg                                                                                                                                        <  7:322739/140‑1 (MQ=255)
      aCGCGGCCTGGGGTATAGCGCGTTAACAGTAGGTGCGCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTgg‑tggt                                                                                                                                      <  5:302676/140‑1 (MQ=255)
             cTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACAGACGAGACGTGCGGGTTGG‑TGGTaataatt                                                                                                                               >  4:957776/1‑139 (MQ=255)
                  ttATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTGCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTc                                                                                                                          <  5:815514/140‑1 (MQ=255)
                  ttATAGAGCGATAACAGTAGGTGCTCTTTACTCGGCGCTTCGCCATTTTCTGCGCTGAGTTATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTc                                                                                                                          <  7:680326/140‑1 (MQ=255)
                    ttaGCGCGGTAACTGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGAGTGATTTCGACGACAGAATGCCTAAAATACGGTCAGAGTCGAGTACTGAGGAGACTTGCGGGTTGG‑TGGTAATAATGGCTTCGt                                                                                                                        <  3:497650/139‑1 (MQ=255)
                    ataGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGt                                                                                                                        <  5:439681/140‑1 (MQ=255)
                    ataGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTAATGTGGAGAATTACGGGTTGG‑TGGTAATAATGGCTTCGt                                                                                                                        >  8:868601/1‑140 (MQ=255)
                      aGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTGGG‑TGGTAATTATGGCTTGGtat                                                                                                                      >  6:856891/1‑138 (MQ=255)
                      aGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACTGAGGCGACGTCCGGGGTGG‑TGGTAATAATGGGTTCGTCt                                                                                                                      >  3:332000/1‑140 (MQ=255)
                      aGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACTGAGGAGACTTGCGGGTTGG‑TGGTAATAATGGCTTCGTCt                                                                                                                      >  2:728537/1‑140 (MQ=255)
                      aGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGACAGAGTCGCGTTCTGAGGGGTCTTCCGGGTTGG‑TGGTTATAATGGGTTCGGCt                                                                                                                      >  4:498758/1‑140 (MQ=255)
                      aGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATAAGGTCAGAGTCGCGTAATGAGGAGACATACGGGGTTG‑TGGTAATAATGGCTTCGTcg                                                                                                                      >  4:213495/1‑139 (MQ=255)
                          cgTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGCATGCCTAAAATACGGTCAGAGTCGCGTCATGAGGAGACTTAGAGGTTGG‑TGGTAATAATGTCTTCGTCTGCaa                                                                                                                  >  2:654714/1‑140 (MQ=255)
                          cgTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTTCTGAGGTGACTTGCGGGTTGG‑GGGTAATAATGGGTTGGTGTGGaa                                                                                                                  >  3:397398/1‑140 (MQ=255)
                          cgTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTTCTGAGGAGACTTCCGGGTTGG‑TGGGAATAATGGCTTAGTCTGGaa                                                                                                                  >  7:118764/1‑140 (MQ=255)
                          cgTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACGTCCGGGTTGG‑TGGTAATAATGGCTTGGTCTGCaa                                                                                                                  >  2:654716/1‑140 (MQ=255)
                          cgTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACTGAGGAGCCTTCCGGGTTGG‑TGGTTATAATTGGTTCGGCTGCaa                                                                                                                  >  7:427533/1‑140 (MQ=255)
                                aaGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGTTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATaga                                                                                                            <  6:120973/139‑1 (MQ=255)
                                  gTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGt                                                                                                          <  6:629522/140‑1 (MQ=255)
                                  gTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGGGATTTCGCCGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGTCGGAGGCGACTTCCGGGGTGG‑TGGTAATAATTGCTTCGTGTGCAAAATATAGt                                                                                                          >  8:738723/1‑140 (MQ=255)
                                     ggggCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGcc                                                                                                       <  1:501502/137‑1 (MQ=255)
                                     ggTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGCCGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTCCTGAGGAGCCTTCCGGGGTTG‑TGGGTATAATGGCTTCGTCTGCAAAATAGAGTggc                                                                                                       >  8:379747/1‑138 (MQ=255)
                                     ggTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTTATAATGTCTTCGTCGGCAAAATATAGTGcc                                                                                                       >  7:831229/1‑140 (MQ=255)
                                      gTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGTCTGAGGAGACTTCCGGGGTGG‑TGGGTATAATTGCTTAGTGTGGAAAATAGAGTGCCa                                                                                                      >  3:88783/1‑140 (MQ=255)
                                         ctctTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGGTGG‑TGGTTATAATGGGTTCGTATGCAAAATAGAGTGCCATTa                                                                                                   >  5:190230/1‑140 (MQ=255)
                                         ctctTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACGGAGGCGACTTCCGGGTTGG‑TGGTTATAATGGCGTAGTGTGCAAAATAGAGTGCCAttt                                                                                                   >  2:157456/1‑139 (MQ=255)
                                         ctctTTAATCGGCTCTTCGACATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTTATGAGGGGCGGTTGGGGTTGGTTGGTAA‑AATGGGGTCGTAGGCAAAATTTAGTGCCaatt                                                                                                   >  6:357394/1‑137 (MQ=255)
                                           ctTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGtaata                                                                                                                                  <  7:245346/107‑1 (MQ=255)
                                           ctTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGtaata                                                                                                                                  >  8:245346/1‑107 (MQ=255)
                                           ctTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGTCAGTGGGGACTTCCCGGTTGG‑TGGTAATAATTGGTTCGTCTGCAAAATAGAGTTcccataaa                                                                                                 >  1:658555/1‑134 (MQ=255)
                                           ctTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACAGGGGAGCCGGCCGGGGTGG‑GGGGAATAATGGCTTCGTCGGGAAAATAGAGGGCCATTAAc                                                                                                 >  4:85799/1‑140 (MQ=255)
                                            tttAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTGACGGGTTGG‑TGGTAATAATGGGTTGGTCTGCCAAATACAGTGCCATTAACg                                                                                                >  8:246132/1‑140 (MQ=255)
                                             ttAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGCGGCTTCCGGGGTGG‑TGGTTATAATGGCTGGGTCGGGAAATTATAGTTGCATTacagc                                                                                               >  8:870128/1‑136 (MQ=255)
                                               aaTCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCAc                                                                                             <  5:859551/140‑1 (MQ=255)
                                               aaTCGGCTCTTCGCCATCTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCAc                                                                                             <  8:116628/140‑1 (MQ=255)
                                                aTCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGGCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCAcc                                                                                            <  7:112591/140‑1 (MQ=255)
                                                    gCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGt                                                                                                                        <  6:420502/108‑1 (MQ=255)
                                                    gCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGGTGG‑TGGTAATAATGGCTTCGt                                                                                                                        >  5:420502/1‑108 (MQ=255)
                                                      tctTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTTCTGAGGAGCCTTCCGGGTTGG‑GGGTTATAATGGGGTCGGCTGGAAAATAGCGGGGCCTTTACGGACCGGtttg                                                                                      >  2:55924/1‑139 (MQ=255)
                                                      tctTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGTGACGTGCGGGTTGG‑TGGTTATAATGGGTTGGTCGGCAAAATATAGTGCCATTAACGCAACGGtttg                                                                                      >  2:158766/1‑139 (MQ=255)
                                                      tctTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGTCGGAGGAGACTTGCGGGGTGG‑TGGTTATTATTGCATAGTATGCAAAATATAGTGCCCTTTACGCTCCGGgttg                                                                                      >  1:399900/1‑139 (MQ=255)
                                                      tctTCGCCATTTTCTGCGCGGCGTGATTTCGACGACAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGCGGAGACTTCCGGGTTGT‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTc                                                                                      <  3:601454/140‑1 (MQ=255)
                                                      tctTCGCCATTTTCTGCGCGGCGTGAGTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTTCTGAGGAGGCTTCCGGGTTGG‑TGGGAATAATGGCTTCGTCTGGAAAATAGAGTGCGATTAACGCCCCGGTTTc                                                                                      >  2:66619/1‑140 (MQ=255)
                                                         tCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAAt                                                                                   <  4:495249/140‑1 (MQ=255)
                                                         gcGCCATTATCTGCGCGGCGTCATTTCTACGCCAGAATGCCTAAAATACGGTCAGAGTAGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTCATAATGGCTTCGTCTTCAAAATAGAGTGCCATTAACGCACCGGTTTCAAt                                                                                   <  1:452851/139‑1 (MQ=255)
                                                                  tCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACTGAGGAGGCTTCCGGGTTGG‑TGGTTATAATTGCTTCGTCTGCAAAATAGAGTGCCATTTACGCACCGGTTTCAATACCTGCCgg                                                                          >  5:616061/1‑140 (MQ=255)
                                                                   cTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCggg                                                                         <  1:377789/140‑1 (MQ=255)
                                                                   cTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGGTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCggg                                                                         <  1:24501/140‑1 (MQ=255)
                                                                      cgcgGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTAATGAGGAGACTTCCGGGTTGG‑TGGTAATAATTGCTTCGTCTGCAAAATAGAGTGGCATTAAAGCACCGTGTTCAATCCCGGCCGGGGAg                                                                      >  2:245034/1‑140 (MQ=255)
                                                                      cgcgGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGTCTGAGGAGACTTGCGGGTTGG‑TGGTAATAATTGCTTCGGCTGCAAAATAGAGTGGCATTAACGCACCGGTTTGAATCCCTTCCGGGGAg                                                                      >  5:842111/1‑140 (MQ=255)
                                                                           cgTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGATGAGCCTTCCGGGGTGG‑TGGTAATAATGGCTTCGTGTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATGCCTGCCGGGGTGTcaaa                                                                 >  2:800856/1‑138 (MQ=255)
                                                                           cgTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTcaca                                                                 <  5:191687/140‑1 (MQ=255)
                                                                           cgTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGGACTGAGGGGGCTTCCGGGGTGG‑TGGGAATAATGGCTTAGGATGCAAAATAGAGTGCCATTAACCCACCGGGGTCAATACCAGCCGGGGGGTcaca                                                                 >  8:616765/1‑140 (MQ=255)
                                                                                        ccAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCa                                                    <  1:54687/140‑1 (MQ=255)
                                                                                         cAGAATGCCTAAAATACGGTCAGAGTCGCGTTCGGAGGAGACGTCCCGGTTGG‑GGGTAATAAAGGCGTCGTTGGGCAAATAGAGTGCCATTAACGCGCCGGGTTCTATCCCGGCCGGGGAGTCCCAAACGATAAATTaaa                                                   >  1:872079/1‑140 (MQ=255)
                                                                                               gCCTAAAATACGGTCCGAGTCGCGTACTGAGGAGACTTCCGGGTAGG‑TGGGAATAATGGCTTCGTCTGCAAAATATAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTAACAAACGATAAATTCAAAATCCa                                             <  8:675304/140‑1 (MQ=255)
                                                                                               gCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCa                                             <  1:622643/140‑1 (MQ=255)
                                                                                               gCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCa                                             <  5:68797/140‑1 (MQ=255)
                                                                                               gCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGGTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCa                                             <  4:736331/140‑1 (MQ=255)
                                                                                               gCCTAAAATACGGGCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCa                                             <  7:578093/140‑1 (MQ=255)
                                                                                                ccTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCGTAGTCTGCAAAATAGATTGCCATTAACGCCCCGGTTTCAAACCCTGCCGGGGAGGGACAAACGATAAATTCAAAATCCaa                                            >  6:852602/1‑139 (MQ=255)
                                                                                                ccTAAAATACGGGCAGAGTCGCTTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCAGAAACGATAAATTCAAATTCCAt                                            <  7:401807/140‑1 (MQ=255)
                                                                                                    aaaTACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCt                                        <  8:107447/140‑1 (MQ=255)
                                                                                                          ggTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGa                                  <  8:220207/140‑1 (MQ=255)
                                                                                                          ggTCAGAGTCGCGTACTGAGGAGACTTACGGGGTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGGCATTAACGCACCGGGTTCAATCCCATCCGGGGTGTCACAAAACATAAATTCAAAATACAACGCTTTAAGa                                  >  8:334935/1‑140 (MQ=255)
                                                                                                          ggTCAGAGTCGCGGACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATTGCGTCGTCTGGAAAATATAGTTCCATTAACGCACCGGGTTCAATCCCAGCCCGGGAGTCACAAACGATAAATTCAAAATACATCGCtttaata                                  >  4:680223/1‑136 (MQ=255)
                                                                                                                 gTCGCGTACAGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCATGCCGGGGAGTCACAAACGATAAATTAAAAATCCATCGCTTTCAGATCATCaa                           <  6:830413/140‑1 (MQ=255)
                                                                                                                  gcgcgTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACTATAAATTCAAAATCCATCGCTTTCAGATCATCAAg                          <  8:775764/139‑1 (MQ=255)
                                                                                                                     cgTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAAc                       <  3:883292/140‑1 (MQ=255)
                                                                                                                     cgTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAAc                       <  5:446867/140‑1 (MQ=255)
                                                                                                                     cgTACTGAGGAGACTTACGGGTTGG‑TGGTAATAAAGGCTTCGTCTGGAAAAAAGAGTGCCATTAAAGCCCCGGTTTCAATACAAGCCGGGGAGTCCCAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAAc                       >  2:456265/1‑140 (MQ=255)
                                                                                                                           gatgagaCTTCCGGGTGGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGGTTCAATCCCTGCCGGGTAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGc                 <  6:39437/137‑1 (MQ=255)
                                                                                                                            aggatACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAACTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCg                <  7:512949/140‑1 (MQ=255)
                                                                                                                            aggagCCTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCg                >  7:823218/1‑140 (MQ=255)
                                                                                                                            aggagACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCg                <  4:616989/140‑1 (MQ=255)
                                                                                                                            aggagACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCg                >  3:703740/1‑140 (MQ=255)
                                                                                                                            aggagACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGACATTAACGCACCGTTTTCAATCCCTGCCGGGTAGTCACAAACGATAAATTCAAAATACATCGATTTCATATCATCAAGAAATTTTGCg                >  2:195496/1‑140 (MQ=255)
                                                                                                                              gagaCTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCAc                                                                                             <  1:111037/61‑1 (MQ=255)
                                                                                                                              gagaCTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAc              <  1:728537/140‑1 (MQ=255)
                                                                                                                              gagaCTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAc              <  6:750488/140‑1 (MQ=255)
                                                                                                                              gagaCTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAc              <  2:940686/140‑1 (MQ=255)
                                                                                                                              gagaCTTCCGGGTTGG‑TGGTAATAATGGCGTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAc              <  6:608469/140‑1 (MQ=255)
                                                                                                                                gaCTTCCGGGTTGG‑TTTTAATAATGGCTTCTTCTGCAAAATAGAGTGCCATTAACGCACCTGTTTCAATCCCTGCCGTGGATTCACAAACGATTAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAccc            <  8:455622/140‑1 (MQ=255)
                                                                                                                                 aCTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAcccc           >  7:567232/1‑140 (MQ=255)
                                                                                                                                 aCTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAcccc           >  2:294565/1‑140 (MQ=255)
                                                                                                                                 aCTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAcccc           >  8:463268/1‑140 (MQ=255)
                                                                                                                                 aCTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCAACGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGAccac           >  4:129608/1‑138 (MQ=255)
                                                                                                                                   ttCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGATTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATAAAGAACTTTGGCGACCCCtt         >  2:814219/1‑140 (MQ=255)
                                                                                                                                     ccGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCa       >  7:66830/1‑140 (MQ=255)
                                                                                                                                     ccGGGTTGC‑TTGTAATAATGGCTGCGGCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCa       <  1:495127/140‑1 (MQ=255)
                                                                                                                                     ccGGGGTGG‑TGGTAATAATGGCATAGTCTGCAAAATAGATTGCCATTAACGCACCGGTTTCAATCCCGGCCGGGGAGTCACAAACTATAAATTCAAAATCCATCGCTGTCAGATCATCAAGAACTTTGGCGACCCCTTCa       <  6:258013/140‑1 (MQ=255)
                                                                                                                                      cGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCa                                             <  5:713653/101‑1 (MQ=255)
                                                                                                                                      cGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCa                                             >  6:713653/1‑101 (MQ=255)
                                                                                                                                      cGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCAc      >  1:11541/1‑140 (MQ=255)
                                                                                                                                      cGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCAc      >  8:151366/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGTAGGTGCTCTTTAATCGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGG‑TGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGT  >  NZ_CP022686/4839549‑4839826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A