breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 42 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,659,0580TC64.7% 5.2 / 13.7 17D38G (GAT→GGT) gadXacid resistance transcriptional activator GadX
*NZ_CP009273653,3500TC61.5% 4.8 / 12.1 13G15G (GGA→GGGcrcBfluoride efflux transporter CrcB
*NZ_CP0092733,348,8160AC59.1% 17.0 / 25.1 22T245P (ACC→CCC) gltBglutamate synthase large subunit
*NZ_CP0092733,273,8300AC58.8% 12.3 / 19.0 17T85T (ACA→ACCagaVPTS N‑acetylgalactosamine transporter subunit IIB
*NZ_CP0092733,659,0620CA58.8% 7.7 / 22.7 17A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
*NZ_CP0092733,977,4500TC58.8% 11.1 / 18.6 17F378S (TTC→TCC) aslBanaerobic sulfatase maturase
*NZ_CP009273262,0600CA58.3% 1.7 / 16.1 12V69F (GTT→TTT) BW25113_RS25425hypothetical protein
*NZ_CP0092733,556,8410TG57.9% 3.7 / 16.6 19V490G (GTG→GGG) glpDglycerol‑3‑phosphate dehydrogenase
*NZ_CP0092733,774,2150TG57.9% 17.4 / 20.8 19G343G (GGT→GGGlldDquinone‑dependent L‑lactate dehydrogenase
*NZ_CP009273353,5910TC56.2% 10.4 / 19.6 16K271E (AAA→GAA) cynRtranscriptional regulator CynR
*NZ_CP009273954,5250TG56.2% 13.7 / 17.8 16G86G (GGT→GGGaroA3‑phosphoshikimate 1‑carboxyvinyltransferase
*NZ_CP009273262,0510TG54.5% 4.6 / 12.4 11T72P (ACC→CCC) BW25113_RS25425hypothetical protein
*NZ_CP009273799,3250GT54.5% 2.7 / 17.5 11V123L (GTG→TTG) ybhJhydratase
*NZ_CP009273976,6890AG54.5% 7.3 / 11.9 11I63V (ATC→GTC) ldtDL,D‑transpeptidase
*NZ_CP0092733,175,9460CG54.5% 3.6 / 10.2 11A13G (GCA→GGA) zupTzinc transporter ZupT
*NZ_CP0092734,594,1610CT54.5% 1.8 / 45.3 44S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP009273997,4470TG53.8% 13.5 / 16.6 13V62G (GTA→GGA) elfGfimbrial protein
*NZ_CP0092733,175,9570AT53.8% 3.8 / 16.0 13I17F (ATT→TTT) zupTzinc transporter ZupT
*NZ_CP009273228,1910AG53.3% 6.6 / 15.8 16S195G (AGC→GGC) yafDendonuclease/exonuclease/phosphatase family protein
*NZ_CP009273700,0260TG53.3% 12.7 / 16.2 16G209G (GGT→GGGnagEPTS N‑acetyl glucosamine transporter subunit IIABC

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 4398991 =52 (2.350)3 (0.170) 3/140 1.6 7.3% intergenic (+79/‑101) rnr/rlmB ribonuclease R/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
?NZ_CP009273 4399052 = 34 (1.870)intergenic (+140/‑40) rnr/rlmB ribonuclease R/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB