breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 83 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,222,9940GT57.1% ‑5.1 / 13.1 28L435M (CTG→ATG) PP_4603 CDS (transposase)
*AE015451_phaM11,327,1210CT53.2% 544.2 / inf 1547intergenic (+97/‑196)PP_16SE rRNA (16S ribosomal RNA)/PP_23SE rRNA (23S ribosomal RNA)–/–
*AE015451_phaM1699,7220AG51.9% 190.0 / inf 847intergenic (+115/‑129)PP_t06 tRNA (tRNA‑Ala)/PP_23SD rRNA (23S ribosomal RNA)–/–
*AE015451_phaM14,348,7050AG48.4% 194.0 / inf 1352intergenic (‑70/+404)PP_3820 CDS (Group II intron‑encoding maturase)/galU CDS (UTP‑glucose‑1‑phosphate uridylyltransferase)–/–
*AE015451_phaM14,344,3120GA45.8% 44.8 / 23.0 93intergenic (+43/‑113)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM15,223,0130CA42.9% ‑6.4 / 24.9 35L428F (TTG→TTTPP_4603 CDS (transposase)
*AE015451_phaM15,737,7560AG40.9% 133.9 / 100.3 209intergenic (+45/+26)hutF CDS (probable formiminoglutamate deiminase)/PP_5037 CDS (lipocalin family lipoprotein)–/–
*AE015451_phaM14,675,3600TG39.1% 208.1 / 47.6 193intergenic (+59/+45)PP_4137 CDS (putative Outer membrane siderophore receptor)/chrR CDS (Chromate reductase)–/–
*AE015451_phaM1195,6340CT37.1% 1245.4 / inf 1006T380T (ACC→ACTPP_0168 CDS (putative surface adhesion protein)
*AE015451_phaM15,485,1530AG32.1% 70.0 / 12.5 55intergenic (+59/‑88)purH CDS (phosphoribosylaminoimidazolecarboxamide formyltransferase_IMP cyclohydrolase)/purD CDS (Phosphoribosylamine‑‑glycine ligase)–/–
*AE015451_phaM15,097,2400AC30.9% 207.8 / 12.9 145intergenic (+231/+61)PP_mr66 ncRNA (P6)/acsA‑I CDS (acetyl‑CoA synthetase)–/–
*AE015451_phaM15,199,6570CT30.1% 88.3 / 12.9 110intergenic (+32/+41)PP_4579 CDS (Transcriptional regulator_ LysR family)/PP_4580 CDS (conserved protein of unknown function)–/–
*AE015451_phaM12,178,5680GA29.3% 2028.9 / inf 1082T268T (ACG→ACAPP_1931 CDS (conserved protein of unknown function)
*AE015451_phaM14,889,6230AG29.1% 221.7 / 23.5 141intergenic (+69/‑84)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
*AE015451_phaM12,241,3050TG28.8% 406.0 / 46.8 285intergenic (+52/‑229)gltX CDS (Glutamate‑‑tRNA ligase)/PP_t25 tRNA (tRNA‑Ala)–/–
*AE015451_phaM15,199,6550CT28.2% 104.7 / 22.0 110intergenic (+30/+43)PP_4579 CDS (Transcriptional regulator_ LysR family)/PP_4580 CDS (conserved protein of unknown function)–/–
*AE015451_phaM14,344,3080GA27.8% 110.1 / 14.3 104intergenic (+39/‑117)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM14,467,6460AG27.7% 1283.2 / 100.0 664intergenic (+27/‑110)nhaA‑II CDS (Na(+)_H(+) antiporter NhaA 2)/PP_3959 CDS (Voltage‑gated chloride channel family protein)–/–
*AE015451_phaM14,889,6440GC27.6% 486.5 / 93.3 238intergenic (+90/‑63)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
*AE015451_phaM15,199,6560GT26.7% 90.9 / 27.8 110intergenic (+31/+42)PP_4579 CDS (Transcriptional regulator_ LysR family)/PP_4580 CDS (conserved protein of unknown function)–/–

Marginal new junction evidence (lowest skew 10 of 304 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451_phaM1 = 1780066236 (0.310)166 (0.230) 64/256 3.4 44.9% intergenic (+25/+81) PP_1587 CDS (NhaP‑type Na+_H+ and K+_H+ antiporters)/dapC CDS (N‑succinyl‑L_L‑diaminopimelate aminotransferase alternative) –/–
?AE015451_phaM1 1780129 = 183 (0.250)intergenic (+88/+18) PP_1587 CDS (NhaP‑type Na+_H+ and K+_H+ antiporters)/dapC CDS (N‑succinyl‑L_L‑diaminopimelate aminotransferase alternative) –/–
* ? AE015451_phaM1 = 555560829 (0.040)233 (0.310)
+GCC
53/264 5.2 89.1% intergenic (‑84/+87) PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–
?AE015451_phaM1 5555609 = 29 (0.040)intergenic (‑85/+86) PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–
* ? AE015451_phaM1 = 2472115276 (0.360)102 (0.140) 49/256 5.5 22.6% intergenic (+19/+130) PP_2166 CDS (Anti‑anti‑sigma factor)/PP_2167 CDS (putative PhoD‑like phosphatase) –/–
?AE015451_phaM1 2472313 = 437 (0.610)coding (1783/1851 nt) PP_2167 CDS (putative PhoD‑like phosphatase)
* ? AE015451_phaM1 4808174 =59 (0.080)195 (0.350) 35/200 5.8 50.2% intergenic (‑283/‑272) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 = 5986211 343 (0.610)intergenic (+229/‑317) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
* ? pS4413_qbsFH 1 =0 (0.000)10375 (0.870) 183/270 5.9 100% intergenic (–/‑1952) –/aadA CDS –/–
?pS4413_qbsFH = 5211 0 (0.000)intergenic (‑521/–) rep CDS/– –/–
* ? AE015451_phaM1 = 652782547 (0.720)112 (0.170) 34/230 7.5 32.4% coding (1961/1962 nt) ctpL CDS (Methyl‑accepting chemotaxis protein CtpL)
?AE015451_phaM1 = 4808159 1 (0.000)intergenic (‑268/‑287) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
* ? pS4413_qbsFH = 8129 (0.000)8028 (0.700)
+GCCGC
157/248 7.8 99.7% intergenic (–/‑1872) –/aadA CDS –/–
?pS4413_qbsFH 82 = 29 (0.000)intergenic (–/‑1871) –/aadA CDS –/–
* ? AE015451_phaM1 = 480817926 (0.030)94 (0.150) 26/230 9.6 30.6% intergenic (‑288/‑267) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 = 5986225 404 (0.630)intergenic (+243/‑303) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
* ? AE015451_phaM1 = 56814153 (0.000)183 (0.250)
+CGGG
31/262 9.7 98.4% intergenic (‑133/+71) PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein) –/–
?AE015451_phaM1 5681416 = 3 (0.000)intergenic (‑134/+70) PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein) –/–
* ? AE015451_phaM1 = 480817926 (0.030)96 (0.140) 28/246 9.8 30.3% intergenic (‑288/‑267) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 5986041 = 417 (0.600)intergenic (+59/‑487) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–