breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,063,3040AC54.2% ‑3.1 / 12.6 27intergenic (+54/+90)aroP_1/CJ019_01001Aromatic amino acid transport protein AroP/hypothetical protein
*ACB1223,440,3630AC53.8% 7.9 / 27.6 39A24A (GCA→GCCCJ019_03266hypothetical protein
*ACB1225,688,6370AC43.8% 24.4 / 12.3 50S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
*ACB1225,384,4080TG35.5% 35.0 / 11.1 31intergenic (+529/‑329)CJ019_05064/CJ019_05067putative protein/hypothetical protein
*ACB1222,730,1660TG33.9% 69.5 / 11.3 62V1336G (GTG→GGG) CJ019_02576hypothetical protein
*ACB1223,063,5210TC32.3% 38.1 / 12.6 40intergenic (+32/‑322)adh_2/pqqD_2Aldehyde dehydrogenase/PqqA binding protein
*ACB1223,138,3670TG32.1% 72.1 / 17.7 56F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
*ACB1221,348,4200CG32.0% 67.2 / 14.3 50D4H (GAC→CAC) CJ019_01261hypothetical protein
*ACB1221,348,4270CG30.4% 72.7 / 12.3 49M1I (ATG→ATC) †CJ019_01261hypothetical protein
*ACB1224,425,0870GC29.0% 51.7 / 11.0 31intergenic (‑29/‑89)CJ019_04200/CJ019_04201hypothetical protein/hypothetical protein
*ACB1225,384,4070CG29.0% 46.9 / 11.1 31intergenic (+528/‑330)CJ019_05064/CJ019_05067putative protein/hypothetical protein
*ACB1221,721,8690GC26.0% 87.0 / 11.8 50V98V (GTC→GTGCJ019_01617hypothetical protein
*ACB1225,356,5120GC25.6% 84.8 / 11.5 43L408V (CTG→GTG) glmMPhosphoglucosamine mutase
*ACB1222,386,9650GT24.3% 64.4 / 11.0 37intergenic (+34/+72)CJ019_02252/dacC_1hypothetical protein/D‑alanyl‑D‑alanine carboxypeptidase DacC
*ACB122434,3760G.22.7% 48.5 / 13.2 22coding (207/1173 nt)bioF8‑amino‑7‑oxononanoate synthase
*ACB1226,038,6580CA21.5% 146.0 / 27.2 93A37S (GCC→TCC) puuD_3Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB122 = 221077955 (0.890)9 (0.150) 8/258 4.6 14.2% coding (345/720 nt) CJ019_02095 hypothetical protein
?ACB122 = 4480292 NA (NA)intergenic (+31/+540) CJ019_03744/recF_2 IS66 family transposase ISPpu14/DNA replication and repair protein RecF
* ? ACB122 = 28301405 (0.080)3 (0.060) 3/220 6.3 31.2% intergenic (‑35/+148) gabD1/namA_3 Succinate‑semialdehyde dehydrogenase [NADP( )]1/NADPH dehydrogenase
?ACB122 2830229 = 9 (0.170)intergenic (‑124/+59) gabD1/namA_3 Succinate‑semialdehyde dehydrogenase [NADP( )]1/NADPH dehydrogenase