breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 232 shown, sorted by frequency from high to low)
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	720,466	0	G	C	56.5%	‑5.3 / 22.1	23	A23P (GCC→CCC)	phrB	deoxyribodipyrimidine photolyase, FAD‑binding
*	REL606	433	0	T	G	54.3%	72.0 / 130.0	81	V33G (GTG→GGG)	thrA	bifunctional aspartokinase I/homeserine dehydrogenase I
*	REL606	2,515,909	0	A	C	50.6%	74.2 / 128.0	79	V260G (GTG→GGG)	ypfI	predicted hydrolase
*	REL606	1,854,401	0	A	C	45.6%	112.8 / 43.7	92	K88N (AAA→AAC)	yeaO	hypothetical protein
*	REL606	4,392,556	0	C	T	45.4%	96.5 / 113.7	130	L43L (CTA→TTA)	aidB	isovaleryl CoA dehydrogenase
*	REL606	2,441,705	0	T	G	42.6%	108.6 / 10.4	68	T139P (ACC→CCC)	ypdG	predicted enzyme IIC component of PTS
*	REL606	4,180,249	0	T	G	41.7%	197.7 / 43.4	120	G24G (GGT→GGG)	yjaH	hypothetical protein
*	REL606	2,735,608	0	G	C	41.4%	29.6 / 13.0	29	A409P (GCC→CCC)	ascF	fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component
*	REL606	2,676,636	0	T	G	41.3%	401.3 / 102.8	244	V149G (GTG→GGG)	smpB	SsrA‑binding protein
*	REL606	931,314	0	T	C	40.3%	117.6 / 59.2	119	E84G (GAG→GGG)	ybjE	predicted transporter
*	REL606	3,472,700	0	C	G	40.0%	80.8 / 86.5	95	A290G (GCG→GGG)	yhgA	predicted transposase
*	REL606	4,554,830	0	A	G	39.5%	118.2 / 24.2	86	intergenic (+30/+27)	yjiV/mcrC	b4486; conserved protein/5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC
*	REL606	2,376,348	0	T	G	38.3%	255.3 / 38.0	150	T63P (ACC→CCC)	folC	bifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*	REL606	3,096,354	0	A	C	37.9%	217.4 / 71.7	140	V163G (GTG→GGG)	sufI	repressor protein for FtsI
*	REL606	3,217,434	0	G	C	37.5%	47.0 / 38.2	72	A76P (GCC→CCC)	agaS	tagatose‑6‑phosphate ketose/aldose isomerase
*	REL606	3,099,091	0	A	C	36.2%	181.5 / 20.4	94	V352G (GTG→GGG)	parC	DNA topoisomerase IV subunit A
*	REL606	3,774,914	0	C	G	35.9%	326.9 / 142.7	184	A20P (GCC→CCC)	yicJ	predicted transporter
*	REL606	4,320,225	0	T	G	34.3%	356.0 / 86.3	176	T40P (ACC→CCC)	melR	DNA‑binding transcriptional dual regulator
*	REL606	2,437,406	0	G	T	34.2%	68.4 / 20.6	38	D435E (GAC→GAA)	ypdD	fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*	REL606	3,783,565	0	A	C	33.6%	216.8 / 61.4	122	T744P (ACA→CCA)	ECB_03521	conserved hypothetical protein

Marginal new junction evidence (sorted from low to high skew)
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	880528 =	260 (1.100)	3 (0.020)	3/122	21.0	1.5%	intergenic (+17/+163)	ybjK/ECB_00814	predicted DNA‑binding transcriptional regulator/conserved hypothetical protein
	?	REL606	= 904682	179 (0.930)					intergenic (+77/+159)	ECB_00851/ybjL	protein similar to prophage P2 OGR protein/hypothetical protein
*	?	REL606	= 324622	235 (0.990)	3 (0.010)	3/136	22.1	1.3%	coding (758/1452 nt)	prpD	2‑methylcitrate dehydratase
	?	REL606	= 3925835	261 (1.220)					coding (197/1485 nt)	gppA	guanosine pentaphosphatase/exopolyphosphatase
*	?	REL606	= 1508528	214 (0.900)	4 (0.020)	3/142	22.5	1.7%	coding (354/846 nt)	nhoA	N‑hydroxyarylamine O‑acetyltransferase
	?	REL606	= 3966877	247 (1.100)					coding (358/381 nt)	yigF	conserved inner membrane protein
*	?	REL606	= 1128081	150 (0.630)	135 (0.570)	3/150	23.1	33.5%	intergenic (+106/‑67)	mdoH/yceK	glucosyltransferase MdoH/predicted lipoprotein
	?	REL606	= 2583282	386 (1.630)					coding (741/741 nt)	yfhQ	predicted methyltransferase