breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,875,4830GC43.5% 95.5 / 184.9 124intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL6063,492,0820TG37.7% 72.3 / 30.3 53V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6061,484,1050AG34.0% 89.6 / 27.7 53G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6061,747,9030GT33.0% 183.0 / 86.1 91A8S (GCA→TCA) ydiLhypothetical protein
*REL6061,795,6500AC31.6% 118.1 / 13.0 57V39G (GTG→GGG) celFcryptic phospho‑beta‑glucosidase, NAD(P)‑binding
*REL6064,393,0610TG28.2% 84.5 / 12.3 39V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6063,691,8440TG27.1% 106.2 / 10.5 48N226T (AAC→ACC) yiaYpredicted Fe‑containing alcohol dehydrogenase
*REL6063,921,7030TG26.5% 148.1 / 11.3 68intergenic (‑88/+111)ppiC/ECB_03654peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/hypothetical protein
*REL6061,688,9900AC26.0% 113.1 / 20.4 50T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*REL606716,8320TG25.0% 117.9 / 11.4 48A31A (GCA→GCCECB_00663hypothetical protein
*REL6061,100,8650AT25.0% 92.4 / 11.3 44Y402F (TAT→TTT) ycdBhypothetical protein
*REL6063,235,4520TG25.0% 64.5 / 10.5 32F85V (TTT→GTT) yhbQhypothetical protein
*REL6064,043,4260TA24.6% 160.5 / 14.6 65I200F (ATT→TTT) yihOpredited transporter
*REL6064,169,2320AT23.5% 169.1 / 14.7 69intergenic (+48/‑165)rpoC/htrCDNA‑directed RNA polymerase subunit beta'/heat shock protein

Marginal new junction evidence (lowest skew 10 of 68 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 352911285 (1.360)4 (0.110)
+30 bp
4/90 5.1 6.5% intergenic (+71/+128) yhhK/livJ hypothetical protein/leucine/isoleucine/valine transporter subunit
?REL606 4353277 = 110 (1.760)intergenic (‑332/+63) yjeJ/yjeK hypothetical protein/predicted lysine aminomutase
* ? REL606 = 352911285 (1.360)4 (0.110)
+30 bp
4/90 5.1 7.4% intergenic (+71/+128) yhhK/livJ hypothetical protein/leucine/isoleucine/valine transporter subunit
?REL606 = 3671450 83 (1.330)intergenic (+36/+115) avtA/yiaI valine‑pyruvate transaminase/predicted hydrogenase, 4Fe‑4S ferredoxin‑type component
* ? REL606 = 352911285 (1.360)4 (0.110)
+30 bp
4/90 5.1 7.6% intergenic (+71/+128) yhhK/livJ hypothetical protein/leucine/isoleucine/valine transporter subunit
?REL606 3569965 = 78 (1.250)intergenic (+135/+109) pitA/yhiO phosphate transporter, low‑affinity/universal stress protein UspB
* ? REL606 2898279 =72 (1.150)4 (0.110)
+30 bp
4/90 5.1 6.9% intergenic (+131/+90) yqeA/yqeB predicted amino acid kinase/conserved protein with NAD(P)‑binding Rossman fold
?REL606 4353277 = 110 (1.760)intergenic (‑332/+63) yjeJ/yjeK hypothetical protein/predicted lysine aminomutase
* ? REL606 2898279 =72 (1.150)4 (0.110)
+30 bp
4/90 5.1 8.0% intergenic (+131/+90) yqeA/yqeB predicted amino acid kinase/conserved protein with NAD(P)‑binding Rossman fold
?REL606 = 3671450 83 (1.330)intergenic (+36/+115) avtA/yiaI valine‑pyruvate transaminase/predicted hydrogenase, 4Fe‑4S ferredoxin‑type component
* ? REL606 2898279 =72 (1.150)4 (0.110)
+30 bp
4/90 5.1 8.2% intergenic (+131/+90) yqeA/yqeB predicted amino acid kinase/conserved protein with NAD(P)‑binding Rossman fold
?REL606 3569965 = 78 (1.250)intergenic (+135/+109) pitA/yhiO phosphate transporter, low‑affinity/universal stress protein UspB
* ? REL606 = 2840545111 (1.770)4 (0.110)
+30 bp
4/90 5.1 6.4% intergenic (‑84/+155) ygdL/mltA hypothetical protein/membrane‑bound lytic murein transglycosylase A
?REL606 = 3529112 85 (1.360)intergenic (+71/+128) yhhK/livJ hypothetical protein/leucine/isoleucine/valine transporter subunit
* ? REL606 = 2840545111 (1.770)4 (0.110)
+30 bp
4/90 5.1 6.8% intergenic (‑84/+155) ygdL/mltA hypothetical protein/membrane‑bound lytic murein transglycosylase A
?REL606 2898279 = 72 (1.150)intergenic (+131/+90) yqeA/yqeB predicted amino acid kinase/conserved protein with NAD(P)‑binding Rossman fold
* ? REL606 2806881 =67 (1.070)4 (0.110)
+30 bp
4/90 5.1 8.1% intergenic (+140/+93) barA/gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/(D)‑glucarate dehydratase 1
?REL606 = 3529112 85 (1.360)intergenic (+71/+128) yhhK/livJ hypothetical protein/leucine/isoleucine/valine transporter subunit
* ? REL606 2806881 =67 (1.070)4 (0.110)
+30 bp
4/90 5.1 8.8% intergenic (+140/+93) barA/gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/(D)‑glucarate dehydratase 1
?REL606 2898279 = 72 (1.150)intergenic (+131/+90) yqeA/yqeB predicted amino acid kinase/conserved protein with NAD(P)‑binding Rossman fold