breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 398,585 | C→A | R220S (CGT→AGT) | secF → | protein export protein SecF |
RA | 422,600 | (G)7→8 | intergenic (‑287/+175) | cyoA ← / ← ampG | cytochrome o ubiquinol oxidase subunit II/muropeptide transporter |
RA | 716,026 | T→C | intergenic (+63/‑133) | ybfC → / → ECB_00662 | hypothetical protein/conserved hypothetical protein |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,250,032 | G→A | S406F (TCT→TTT) | infB ← | translation initiation factor IF‑2 |
RA | 3,489,216 | C→T | intergenic (‑10/+7) | glpR ← / ← glpG | DNA‑binding transcriptional repressor/predicted intramembrane serine protease |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
RA | 3,928,436 | G→A | A74T (GCC→ACC) | rho → | transcription termination factor Rho |
RA | 4,162,470 | A→C | T539P (ACC→CCC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,163,089 | A→C | E745A (GAA→GCA) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,202,462 | A→G | L33S (TTA→TCA) | iclR ← | DNA‑binding transcriptional repressor |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 3895000 | 3901930 | 6931 | 19 [0] | [0] 63 | rbsD–[yieO] | rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 3652533 | NA (NA) | 60 (1.100) | 43/146 | 0.2 | 100% | noncoding (1443/1443 nt) | IS150 | repeat region |
? | REL606 | 3901931 = | 0 (0.000) | coding (489/1428 nt) | yieO | predicted multidrug or homocysteine efflux system |