breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 398,585 C→A R220S (CGT→AGT)  secF → protein export protein SecF
RA 422,600 (G)7→8 intergenic (‑287/+175) cyoA ← / ← ampG cytochrome o ubiquinol oxidase subunit II/muropeptide transporter
RA 716,026 T→C intergenic (+63/‑133) ybfC → / → ECB_00662 hypothetical protein/conserved hypothetical protein
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,250,032 G→A S406F (TCT→TTT)  infB ← translation initiation factor IF‑2
RA 3,489,216 C→T intergenic (‑10/+7) glpR ← / ← glpG DNA‑binding transcriptional repressor/predicted intramembrane serine protease
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
RA 3,928,436 G→A A74T (GCC→ACC)  rho → transcription termination factor Rho
RA 4,162,470 A→C T539P (ACC→CCC)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,163,089 A→C E745A (GAA→GCA)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,202,462 A→G L33S (TTA→TCA)  iclR ← DNA‑binding transcriptional repressor
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3895000 3901930 6931 19 [0] [0] 63 rbsD–[yieO] rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 3652533NA (NA)60 (1.100) 43/146 0.2 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 3901931 = 0 (0.000)coding (489/1428 nt) yieO predicted multidrug or homocysteine efflux system