breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 382,261 | A→G | I639T (ATT→ACT) | sbcC ← | exonuclease, dsDNA, ATP‑dependent |
RA | 1,004,196 | T→A | intergenic (‑122/+480) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
RA | 1,290,813 | C→T | Q260* (CAG→TAG) | hnr → | response regulator of RpoS |
RA | 1,304,966 | C→T | R425Q (CGG→CAG) | cls ← | cardiolipin synthetase |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,228,283 | G→T | P96Q (CCG→CAG) | yraL ← | predicted methyltransferase |
RA | 3,499,829 | G→T | F325L (TTC→TTA) | glgC ← | glucose‑1‑phosphate adenylyltransferase |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 4,162,569 | A→C | I572L (ATC→CTC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,438,531 | T→G | D110A (GAC→GCC) | fbp ← | fructose‑1,6‑bisphosphatase |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 546990–547700 | 573919 | 26220–26930 | 16 [15] | [15] 16 | [insB‑6]–ECB_00530 | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530 |
* | * | ÷ | REL606 | 573958 | 593387 | 19430 | 16 [14] | [15] 16 | cusS–[fepA] | cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
* | * | ÷ | REL606 | 593577 | 619822–619116 | 25540–26246 | 16 [15] | [13] 16 | [fepA]–[insA‑8] | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 2774196 | NA (NA) | 45 (0.750) | 30/144 | 1.0 | 51.1% | noncoding (1343/1343 nt) | IS186 | repeat region |
? | REL606 | 4210241 = | 43 (0.720) | intergenic (+84/+49) | yjbC/yjbD | 23S rRNA pseudouridine synthase/hypothetical protein |