breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 42,119 C→T 8.3% intergenic (‑188/‑284) caiT ← / → fixA putative transporter/anaerobic carnitine reduction putative electron transfer flavoprotein subunit
RA 349,673 G→C 5.1% intergenic (+101/‑339) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 349,740 C→A 5.0% intergenic (+168/‑272) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 375,176 A→G 6.1% intergenic (+295/‑283) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 1,111,641 C→T 6.4% A260V (GCT→GTT)  opgH → OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
RA 1,286,965 A→G 11.4% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,334,390 C→A 11.1% A179E (GCA→GAA)  cysB → N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor
RA 1,413,542 A→T 100% Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
RA 1,708,844 C→G 8.9% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,793,897 Δ1 bp 100% coding (213/552 nt) btuE ← glutathione peroxidase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,093,040 G→A 100% A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase
RA 2,159,421 A→T 15.7% I346F (ATT→TTT)  mdtC → multidrug efflux system, subunit C
RA 2,304,796 T→C 11.6% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,473,882 T→G 11.7% L94R (CTG→CGG)  yfdP → CPS‑53 (KpLE1) prophage; uncharacterized protein
RA 2,537,741 G→A 9.9% intergenic (+27/‑6) yfeK → / → yfeS uncharacterized protein/WGR domain protein
RA 2,553,175 T→A 5.7% F276I (TTC→ATC)  amiA → N‑acetylmuramoyl‑l‑alanine amidase I
RA 2,897,558 G→A 7.5% A104V (GCC→GTC)  ygcS ← putative MFS sugar transporter; membrane protein
RA 3,577,084 C→T 7.0% V170I (GTT→ATT)  gntK ← gluconate kinase 2
RA 3,587,084 T→A 5.4% I48N (ATC→AAC)  yhhA → DUF2756 family protein
RA 3,836,954 G→A 9.4% M1M (ATG→ATA) † setC → putative arabinose efflux transporter
RA 3,917,417 C→A 7.9% G283V (GGG→GTG)  atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit
RA 3,935,065 C→A 5.6% A430E (GCG→GAG)  rbsA → D‑ribose ABC transporter ATPase
RA 4,117,109 T→G 94.7% Q38P (CAA→CCA)  glpK ← glycerol kinase
RA 4,187,597 C→T 100% P750L (CCG→CTG)  rpoC → RNA polymerase, beta prime subunit
RA 4,578,192 G→T 5.6% Q383K (CAA→AAA)  mcrB ← 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)80 (0.830) 61/260 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
* ? NC_000913 = 78168889 (0.870)4 (0.060) 4/168 NT 7.1% intergenic (+36/‑397) lysQ/nadA tRNA‑Lys/quinolinate synthase, subunit A
?NC_000913 781845 = 51 (0.820)intergenic (+193/‑240) lysQ/nadA tRNA‑Lys/quinolinate synthase, subunit A
* ? NC_000913 1207790 =19 (0.190)57 (0.630) 45/244 NT 77.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 17 (0.190)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780520 (0.200)57 (0.630) 51/244 NT 76.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 17 (0.190)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)87 (0.880) 70/268 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein