breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 289 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,320,6500AG62.7% 543.9 / inf 595R129G (AGA→GGA) thiEthiamine phosphate synthase
*DH14,335,4690AG62.3% 563.8 / inf 626S41S (AGT→AGCrplA50S ribosomal protein L1
*DH14,338,6430AG60.6% 544.7 / inf 701noncoding (74/76 nt)ECDH1_RS21430tRNA‑Thr
*DH1755,7820TC60.6% 419.1 / inf 589L14P (CTC→CCC) glcDFAD‑binding protein
*DH13,184,6490AG59.8% 174.5 / inf 367noncoding (35/75 nt)ECDH1_RS15800tRNA‑TERM
*DH11,983,6390GA59.6% 434.0 / inf 545P12L (CCC→CTC) pabBaminodeoxychorismate synthase subunit I
*DH12,102,3780TC59.5% 352.3 / inf 510K172E (AAA→GAA) ydiFhypothetical protein
*DH11,537,8100AG59.5% 357.4 / inf 534N87D (AAC→GAC) gyrADNA gyrase subunit A
*DH14,078,6810AG59.5% 323.1 / inf 497pseudogene (1442/1959 nt)cpdB2', 3'‑cyclic nucleotide 2'‑phosphodiesterase
*DH11,743,5580TC59.5% 371.5 / inf 554V385V (GTT→GTCwzctyrosine‑protein kinase wzc
*DH14,335,5870AG59.4% 419.9 / inf 609V2A (GTT→GCT) rplA50S ribosomal protein L1
*DH12,424,4860TC59.4% 357.4 / inf 531intergenic (‑1/‑350)paaA/paaZphenylacetate‑CoA oxygenase subunit PaaA/phenylacetic acid degradation bifunctional protein PaaZ
*DH14,026,7830AG59.2% 371.0 / inf 568C269R (TGT→CGT) lptFLPS export ABC transporter permease LptF
*DH12,709,9030CT59.2% 387.5 / inf 539P179L (CCG→CTG) ldtCpeptidoglycan‑binding protein LysM
*DH12,580,9820TC59.0% 302.5 / inf 469V209A (GTT→GCT) adhEbifunctional acetaldehyde‑CoA/alcohol dehydrogenase
*DH14,235,9820AG59.0% 358.8 / inf 553S4P (TCA→CCA) ghxPpermease
*DH14,429,5380AC58.9% 514.9 / inf 526L85F (TTA→TTCfdoGformate dehydrogenase‑N subunit alpha
*DH14,289,9870AG58.8% 321.2 / inf 506F219S (TTC→TCC) metHmethionine synthase
*DH12,688,0950AG58.8% 319.8 / inf 502K115E (AAA→GAA) purBadenylosuccinate lyase
*DH11,883,4180CT58.6% 399.9 / inf 581H24Y (CAC→TAC) uvrCexcinuclease ABC subunit C

Marginal new junction evidence (lowest skew 10 of 63 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 67831256 (0.450)270 (0.510) 168/280 3.3 51.1% coding (619/717 nt) rph ribonuclease PH
?DH1 67915 = 277 (0.520)coding (703/717 nt) rph ribonuclease PH
* ? DH1 = 300335NA (NA)303 (0.530) 173/300 4.2 54.0% noncoding (772/773 nt) repeat_region repeat region
?DH1 = 1971083 258 (0.450)coding (41/210 nt) cspC cold‑shock protein CspC
* ? DH1 = 2201911271 (0.480)250 (0.440) 168/300 4.9 61.1% coding (1337/1533 nt) pntA NAD(P) transhydrogenase subunit alpha
?DH1 2204947 = 47 (0.080)intergenic (‑382/‑30) tqsA/mdtJ AI‑2 transporter TqsA/spermidine export protein MdtJ
* ? DH1 = 300335NA (NA)239 (0.420) 160/300 6.1 39.8% noncoding (772/773 nt) repeat_region repeat region
?DH1 490607 = 362 (0.640)coding (3102/3801 nt) yhdP DUF3971 domain‑containing protein
* ? DH1 299568 =NA (NA)262 (0.460) 157/300 6.6 41.5% noncoding (5/773 nt) repeat_region repeat region
?DH1 = 490615 369 (0.650)coding (3110/3801 nt) yhdP DUF3971 domain‑containing protein
* ? DH1 3203897 =372 (0.650)233 (0.430) 147/284 6.7 39.8% coding (512/708 nt) ybeR hypothetical protein
?DH1 = 3203926 354 (0.660)coding (483/708 nt) ybeR hypothetical protein
* ? DH1 = 2991630309 (0.550)376 (0.670) 152/298 7.2 54.9% coding (331/1686 nt) ybjL transporter
?DH1 = 3577068 NA (NA)noncoding (1198/1201 nt) repeat_region repeat region
* ? DH1 = 1971081251 (0.450)285 (0.510) 148/296 7.7 53.2% coding (39/210 nt) cspC cold‑shock protein CspC
?DH1 3580751 = NA (NA)noncoding (1/976 nt) repeat_region repeat region
* ? DH1 2991627 =334 (0.590)394 (0.690) 150/300 7.8 54.1% coding (328/1686 nt) ybjL transporter
?DH1 3575874 = NA (NA)noncoding (4/1201 nt) repeat_region repeat region
* ? DH1 = 4493676321 (0.560)236 (0.410) 149/300 8.0 42.6% coding (569/1641 nt) ubiB ubiquinone biosynthesis protein UbiB
?DH1 4493689 = 316 (0.560)coding (556/1641 nt) ubiB ubiquinone biosynthesis protein UbiB