breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 124,241 C→T Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 461,007 T→G I707S (ATC→AGC)  lon → Lon protease
JC 607,712 Δ7 bp intergenic (‑329/‑16) ybdK ← / → sokE carboxylate‑amine ligase/small RNA SokE
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 754,446 A→G L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,368,101 T→C A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,661,821 C→G C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
MC JC 2,717,178 Δ3,274 bp [ung][patZ] [ung], yfiF, trxC, yfiP, [patZ]
RA 3,041,634 A→C T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,473,614 C→T W156* (TGG→TGA rpsG ← 30S ribosomal subunit protein S7
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp [rph] [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423736–3424234 3424528–3424238 5–793 21 [19] [20] 22 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 566777 =NA (NA)3120 (57.830) 271/280 0.0 98.2% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 58 (1.070)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 = 57247158 (1.070)17 (0.410)
+34 bp
12/214 2.6 33.0% coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
?NC_000913 = 2170235 33 (0.610)noncoding (65/1258 nt) IS3 repeat region
* ? NC_000913 1436096 =81 (1.490)282 (5.230) 161/280 0.0 82.2% coding (798/1134 nt) ompN outer membrane porin N
?NC_000913 = 1441187 42 (0.780)coding (130/267 nt) ydbJ DUF333 domain‑containing protein YdbJ